Differentiation of Hedyotis diffusa and Common Adulterants Based on Chloroplast Genome Sequencing and DNA Barcoding Markers

Chinese herbal tea, also known as Liang Cha or cooling beverage, is popular in South China. It is regarded as a quick-fix remedy to relieve minor health problems. Hedyotis diffusa Willd. (colloquially Baihuasheshecao) is a common ingredient of cooling beverages. H. diffusa is also used to treat cancer and bacterial infections. Owing to the high demand for H. diffusa, two common adulterants, Hedyotis brachypoda (DC.) Sivar and Biju (colloquially Nidingjingcao) and Hedyotis corymbosa (L.) Lam. (colloquially Shuixiancao), are commonly encountered in the market. Owing to the close similarity of their morphological characteristics, it is difficult to differentiate them. Here, we sequenced the complete chloroplast genomes of the three species of Hedyotis using next-generation sequencing (NGS). By comparing the complete chloroplast genomes, we found that they are closely related in the subfamily Rubioideae. We also discovered that there are significant differences in the number and repeating motifs of microsatellites and complex repeats and revealed three divergent hotspots, rps16-trnQ intergenic spacer, ndhD and ycf1. By using these species-specific sequences, we propose new DNA barcoding markers for the authentication of H. diffusa and its two common adulterants.

Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea

Searching for natural and safe herbal tea with health benefits has attracted more and more attention, which is of great significance for reducing disease risk. A Chinese traditional herbal tea (HT) is rich in active ingredients extracted from natural plants. Numerous pharmacological studies showed that HT had the potential to improve health, including antidepression and antioxidant effects. In this study, we proposed a strategy to explore the role and underlying mechanism of HT in improving healthspan of a Caenorhabditis elegans model. First, we found that HT significantly prolonged the lifespan without reducing fertility in worms. Second, stress resistance (oxidative stress and heat stress) was enhanced and Aβ- and polyQ-induced toxicity was relieved significantly by HT treatment. Both fat deposition and age pigment accumulation were found to be significantly reduced in HT-treated worms. The locomotion in mid-late stages was improved, indicating that behavioral mobility was also significantly enhanced. Furthermore, the main components of HT were eighteen polyphenols and two terpenoids. Finally, it was found that this protective mechanism was positively correlated with the insulin/insulin-like growth factor signaling- (IIS-) dependent manner, which went through promoting the nuclear localization of DAF-16 and its downstream SOD-3 expression. These results suggested that HT had an important role in improving health, which might serve as a promising healthy tea.

Consumers’ knowledge and attitude towards Chinese herbal tea and consumption of Chinese herbal tea in selected district in Kedah

Interest in botanical remedies has attracted growing attention in Chinese herbal teas among industry, scientists and consumers. A survey was conducted among 203 respondents recruited from several Chinese herbal shops in a selected town in Kedah. Data were subjected to obtain descriptive statistics and inferential tests. The results showed that more than half of consumers have moderate knowledge level and almost positive attitude towards Chinese herbal tea. Consumers of different races have a significant difference in knowledge score towards Chinese herbal tea. In addition, consumers’ attitude between different age groups was also found to be different. Consumers’ gender, race, age, marital status, and employment status were associated with their Chinese herbal tea consumption. This study revealed public’ understanding towards knowledge, attitude and consumption towards Chinese herbal tea. It is anticipated that these findings may benefit authorities in policies and regulations development and convey correct information and useful knowledge for the public as well as Chinese herbal tea traders for strategic marketing to meet consumers’ demand.