Previous herbivory modulates aphid population growth and plant defense responses in a non-model plant, Carthamus tinctorius (Asteraceae)

Plants have a variety of defense mechanisms that are often induced following attacks by herbivores; this benefits those plants by decreasing performance or preference of herbivores that attack the plants later. We investigated the effects of previous exposure of plants to the safflower aphid, Uroleucon carthami, cotton bollworm, Helicoverpa armigera, and mechanical wounding on subsequent safflower aphid infestations using commercial safflower (Carthamus tinctorius) cultivars and wild safflower species (C. oxyacantha). The experiments were conducted in a greenhouse with two treatments: previously induced plants via direct herbivory or mechanical wounding, and control plants that had never experienced herbivory. To test the performance of safflower aphid on different plant treatments, five unwinged aphids were placed on each plant and allowed to reproduce for 14 days. Finally, the total numbers of aphids on each plant were counted and the percentage of produced winged individuals was calculated. The number of aphids on plants that were previously infested or injured was significantly lower than in control plants. Percentage of winged aphids was significantly higher on induced plants, which is an indicator for unsuitable conditions. Also, significant increase in total phenolic content and hydrogen peroxide was observed in induced plants, showing that the levels of these compounds were either treatment, cultivar and/or genotype × treatment dependent, highlighting the specificity of these interactions. Overall, among the safflower cultivars the lowest number of aphids and the highest percentage of winged aphid individuals were observed on Mahali-Isfahan cultivar and wild safflower, showing that this cultivar is more sensitive to herbivory and/or responds to it more than other cultivars. These findings could contribute to a better utilization of induced defense in the integrated pest management of safflower fields.

Isolation and Evaluation of Antibacterial Potential Test of Plant Carthamus oxycantha

The present investigation was initiated to find a suitable alternative to synthetic antibiotics for the management of diseases caused by bacteria. Carthamus oxycantha.L locally known as wild safflower member of family Asteraceae that grows wildly. The study was conducted using as Agar well diffusion to trace the antibacterial potential for to evaluate the efficiency of ethanolic extract of Carthamus oxycantha with concentration of 05, 10, 15, and 20 mg/ml against gram-positive Staphylococcus aureus and gram-negative Escherichia Coli species and them compared with that of Clindamycin, Ampicillin and Kanamycin (10 mg). Zone of inhibition for the extracts were 10.667 to 20.00 mm as compared to standard drug Clindamycin, Ampicillin and kanamycin (15.00-20.00 mm). Antibacterial assays indicates that Carthamus oxycantha has potential natural antimicrobial agents against E-coli and S. aureus. The findings of the present study suggested that ethanolic extract of C. oxycantha has strong potential to serve as possible antibacterial.

Broadleaf weed control in rain-fed chickpea

Weeds are among the main limitations on chickpea production in Iran. The efficacy of herbicide treatments including linuron PPI, imazethapyr PPI, PRE, and POST, pendimethalin PPI and POST, bentazon POST, pyridate POST, and oxadiazon POST along with one or two hand weedings were evaluated for weed control and yield response in rain-fed chickpea in Aleshtar, Lorestan, Iran in 2015 and 2016. Wild safflower, threehorn bedstraw, wild mustard, and hoary cress were the predominant weed species in both experimental years. Total weed dry biomass in weedy check plots averaged 187 and 238 g m−2 in 2015 and 2016, respectively, and weed density and biomass were reduced in all treatments compared to the weedy check in both years. Treatments composed of pyridate followed by one hand weeding or imazethapyr POST followed by two hand weedings resulted in the lowest weed biomass. The presence of weeds reduced yield by 74% and 66% in the weedy check plots compared to the weed-free control plots in 2015 and 2016, respectively. Application of oxadiazon, bentazon, and imazethapyr PPI, PRE, and POST resulted in lower chickpea yields. All herbicides tested injured chickpea slightly, with pyridate causing the least injury.

Efficacy of Five Herbicides for Weed Control in Rain-Fed Lentil (Lens culinaris Medik.)

Lentil is vulnerable to weed competition because of its short stature, slow establishment, and limited vegetative growth. Although the vast majority of lentil production is under rain-fed conditions, there is a little published information on weed control with herbicides in rain-fed lentils. Field experiments were conducted to determine the efficacy of nine herbicide treatments including fomesafen, imazethapyr, linuron, pendimethalin, and pyridate alone or in combination compared with one or two hand weeding(s) on weed control and yield response in rain-fed lentil in Khorramabad, Iran in 2012 and 2013. Weed species included catchweed bedstraw, cowcockle, haresear mustard, hoary cress, wild mustard, and wild safflower. Total weed dry biomass in weedy check plots averaged 156 and 170 g m−2 in 2012 and 2013, respectively, and weed density and biomass were reduced in all treatments compared to the weedy check in both years. Plots that were hand weeded twice reduced weed biomass the greatest, whereas fomesafen, linuron, or one hand weeding did not control weeds satisfactorily. Noncontrolled weeds reduced lentil yield by 67% both years compared to the weed-free control. Lentil yield in 2013 (1,370 kg ha−1) was higher than in 2012 (1,150 kg ha−1). All herbicides tested injured lentil slightly, with pyridate (1,200 g ai ha−1) and pendimethalin (660 g ai ha−1 plus imazethapyr at 250 to 500 g ai ha−1) causing the least injury. Across all treatments, imazethapyr plus pendimethalin PRE, pyridate POST, and two hand-weeding treatments had the best performance for weed control and lentil yield.