The role of glycation end products in the development and progression of diabetic neuroarthropathy

Diabetic neuroarthropathy (DNOAP, Charcot’s foot) is a serious complication of diabetes mellitus, the genesis of which is not fully understood. In most cases, this pathology is diagnosed late, which leads to the development of severe deformities of the foot, up to the loss of support ability of the limb. There is no single hypothesis for the formation of Charcot’s foot, but there are factors predisposing to its development, as well as a few likely provoking events. Excessive formation and accumulation of end products of glycation may play an important role in the pathogenesis of this complication of diabetes. End products of glycation (AGE) are a variety of compounds formed as a result of a non-enzymatic reaction between carbohydrates and free amino groups of proteins, lipids and nucleic acids. There are various factors that lead to the accumulation of AGE in the human body. Allocate endogenous and exogenous factors. The former include certain diseases, such as diabetes mellitus, renal failure, which accelerate glycation processes. Exogenous factors leading to the formation of lipo-oxidation and glyco-oxidation products include tobacco smoke and prolonged heat treatment of food.This review provides information on the role of glycation end products in the development and progression of complications in patients with diabetes mellitus.

PROLONGED HEAT TREATMENT FOR THE CONDUCTIVITY OF ALUMINUM-MAGNESIUM CERAMICS

In their work they tried to reveal the use of oxide ceramics for high- temperature insulation and conductive materials. The effect of prolonged temperature on its electrical conductivity was studied. The table shows the physical and technical properties of ceramics after additional processing at a temperature of 1700°С.

Researches in the field of composite HCBS and of the refractory materials on their base in the Al2O3‒SiO2‒SiC system. Part 2

The influence was investigated of the prolonged heat treatment on the samples properties prepared on base of the composite Al2O3‒SiO2‒SiC HCBS. The dispersion degree of the samples containing either 10 or 30 % of SiC varied within 3‒200 microns. The estimating method was proposed for the SiC oxidation degree in the composite. Depending on the samples composition the oxidation degree was within 50‒100 %. The ultimate bend strength and compressing strength SiC-containing samples attained the values of 80 and 390 MPa respectively. `````````````````````````````

Diffusion Bonding of Ceramics by Spark Plasma Sintering (SPS) Apparatus

Alumina, Silicon Carbide, Boron Carbide and Magnesium Aluminate Spinel were directly joined by a Spark Plasma Sintering (SPS) apparatus. The optimal parameters for joining (temperature, holding time and applied pressure) were experimentally found. The joined regions were investigated using scanning electron microscopy (SEM) ultrasonic and micro hardness testing. Alumina, Boron Carbide and Silicon Carbide parts were successfully joined at 1300, 1800 and 1900°C, respectively, for 30 min under argon atmosphere (10- 2 torr) and 40 MPa uniaxial pressure. Spinel parts were successfully joined at 1300°C for 60 min. Micro hardness of the interfacial regions were similar to those of the bonded specimens. The effect of a prolonged heat treatment on the microstructural evolution of the joined regions was investigated and the grain growth mechanism was discussed.

Synthesis of TiO2 and F-Doping TiO2 Powders via "Reverse" Hydrolysis of Titanium Tetraisopropoxide

TiO2 and F-TiO2 powders were prepared by hydrolysis of titanium tetraisopropoxide with reversed mixing of different hydrolytic reagents. The typeof the hydrolytic agentstronglyinfluences particle size, morphology and phase composition ofpowder.It was found, that the amount of injected water is significantly affects on BET surface area and particle size ofbare TiO2.The presence offluorinemakes the samplemore porous and preventsanatase to rutile phase transformation under prolonged heat treatment, also particle formed are bigger in comparison with un-doped TiO2.

Expression Patterns of Convergently Overlapping Arabidopsis Thaliana Gene Pairs OHP-NDP1 and OHP2-MES14

The function of one-helix proteins (OHPs) in the thylakoid membrane remains poorly understood but may be linked to plant photosystem protection. In Arabidopsis, the 3'UTRs of the genes encoding OHP and OHP2 partially overlap with NDP1 and MES14 respectively. Antisense orientation of genes has the potential to form double-stranded transcript (dsRNA) molecules which can be processed to siRNA and trigger RNA interference (RNAi). Natural siRNAs are induced by abiotic and biotic stresses. We examined whether the expression of the OHP-NDP1 and OHP2-MES14 gene pairs is regulated in this way. Both OHP genes, but neither NDP1 nor MES14, were activated by light in etiolated seedlings, whereas cold and prolonged heat treatment elevated the OHP transcript level. Expression of OHP2 was down-regulated after 2 h of osmotic and heat stress, while salt and osmotic stress increased MES14 transcript levels. No inverse regulation of these overlapping gene pairs was observed, excluding RNAi as a regulatory mechanism in the tested conditions. The presence of alternatively polyadenylat-ed transcripts of the studied genes raises the possibility of another regulatory mechanism of 3'UTR overlap.

Effect of Time and Temperature on PrPCWD Immunoreactivity as Evidenced by Western Blot

The protease-resistant infectious prion protein, PrPres, that causes transmissible spongiform encephalopathies, is remarkably resistant to conventional physical and chemical sterilization methods, including heat. It was hypothesized that thermal-dependent PrPres degradation has been underestimated, and the effect of prolonged incubation at 37°C, 55°C, and 80°C on PrPres detection was examined using brain homogenates from chronic wasting disease-affected elk and mule deer (PrPCWD). Immunoblotting demonstrated progressive loss of PrPCWD immunoreactivity with time in all incubated samples as temperature increased, and PrPCWD was virtually undetectable after 90 days of incubation at 55°C and 80°C. These results indicate that decontamination methods and tissue disposal systems maintaining elevated temperatures for long periods of time could interfere with immunodetection, and the reliability of assays for PrPres detection could be compromised when applied to tissues exposed to heat with time. Although these results may suggest that such prolonged heat treatment could destroy prions, the observed loss of immunoreactivity does not necessarily correlate with a concurrent loss of infectivity. Bioassay is needed to determine if samples that have been incubated under these conditions retain infectivity.