Density- and elongation speed-dependent error correction in RNA polymerization

Backtracking of RNA polymerase (RNAP) is an important pausing mechanism during DNA transcription that is part of the error correction process that enhances transcription fidelity. We model the backtracking mechanism of RNA polymerase, which usually happens when the polymerase tries to incorporate a noncognate or "mismatched" nucleotide triphosphate. Previous models have made simplifying assumptions such as neglecting the trailing polymerase behind the backtracking polymerase or assuming that the trailing polymerase is stationary. We derive exact analytic solutions of a stochastic model that includes locally interacting RNAPs by explicitly showing how a trailing RNAP influences the probability that an error is corrected or incorporated by the leading backtracking RNAP. We also provide two related methods for computing the mean times for error correction and incorporation given an initial local RNAP configuration. Using these results, we propose an effective interacting-RNAP lattice that can be readily simulated.

NETWORK DGPS ACCURACY ASSESSMENT AT WEST COAST PENINSULAR MALAYSIA

Maritime safety of navigation is essential for maritime activities especially when approaching the harbor. One of the important aspects in safety of navigation is positioning accuracy which the accuracy should less than 5 meters as recommended by IALA. Such accuracy can be provided by DGPS services. Nevertheless, the DGPS accuracy is bound to distance-dependent error due to uncorrelated errors between reference and rover station. By implementing the network-based DGPS technique, this issue however can be expected to be improved. This issue can be overcome by implementing the network-based DGPS technique to the positioning method. Hence, multiple of CORS in Peninsular Malaysia and Sumatran Indonesian were utilized to generate the network-based DGPS corrections based on LIM to cover the west coast of Peninsular Malaysia. The single point positioning, DGPS and network-based DGPS solutions were being compared with known points to determine the reliability of positioning in marine activities. The accuracy shows that the DGPS and network-based DGPS are better compared to single point positioning with below than 15m and 20m respectively. Meanwhile, the DGPS technique clearly had shown the distance-dependent error propagation in positioning. This paper presents on the accuracy and efficiency of network DGPS technique in reducing the distance-dependent error in DGPS positioning.

BUNDLE ADJUSTMENT WITH POLYNOMIAL POINT-TO-CAMERA DISTANCE DEPENDENT CORRECTIONS FOR UNDERWATER PHOTOGRAMMETRY

Uncontrolled refraction of optical rays in underwater photogrammetry is known to reduce its accuracy potential. Several strategies have been proposed aiming at restoring the accuracy to levels comparable with photogrammetry applied in air. These methods are mainly based on rigours modelling of the refraction phenomenon or empirical iterative refraction corrections. The authors of this contribution have proposed two mitigation strategies of image residuals systematic patterns in the image plane: (i) empirical weighting of image observations as function of their radial position; (ii) iterative look-up table corrections computed in a squared grid. Here, a novel approach is developed. It explicitly takes into account the object point-to-camera distance dependent error introduced by refraction in multimedia photogrammetry. A polynomial correction function is iteratively computed to correct the image residuals clustered in radial slices in the image plane as function of the point-to-camera distance. The effectiveness of the proposed method is demonstrated by simulations that allow to: (i) separate the geometric error under investigation from other effects not easily modellable and (ii) have reliable reference data against which to assess the accuracy of the result.

Data Assimilation of Satellite-Based Soil Moisture into a Distributed Hydrological Model for Streamflow Predictions

The authors examine the impact of assimilating satellite-based soil moisture estimates on real-time streamflow predictions made by the distributed hydrologic model HLM. They use SMAP (Soil Moisture Active Passive) and SMOS (Soil Moisture Ocean Salinity) data in an agricultural region of the state of Iowa in the central U.S. They explore three different strategies for updating model soil moisture states using satellite-based soil moisture observations. The first is a “hard update” method equivalent to replacing the model soil moisture with satellite observed soil moisture. The second is Ensemble Kalman Filter (EnKF) to update the model soil moisture, accounting for modeling and observational errors. The third strategy introduces a time-dependent error variance model of satellite-based soil moisture observations for perturbation of EnKF. The study compares streamflow predictions with 131 USGS gauge observations for four years (2015–2018). The results indicate that assimilating satellite-based soil moisture using EnKF reduces predicted peak error compared to that from the open-loop and hard update data assimilation. Furthermore, the inclusion of the time-dependent error variance model in EnKF improves overall streamflow prediction performance. Implications of the study are useful for the application of satellite soil moisture for operational real-time streamflow forecasting.