EFFECT OF PENICILLIN -CIPROFLOXACIN COMBINATION ON SEMEN CHARACTERISTIC AND BACTERIAL CONTAMINANTS OF DILUTED BULL SEMEN

Effect of penicillin- ciprofloxacin combinations on some semen characteristics and bacterial contaminants of diluted bull semen was determined and compared with those of penicillin- streptomycin combinations. Sperm motility, percentage of dead and abnormal spermatozoa and survival index did not differ significantly for penicillin (1000 IU) and ciprofloxacin (125, 250, 500, 1000 ug) combinations per milliliter of diluted bull semen from those of penicillin (1000 I.U) and streptomycin (500 ug) combination / ml. Each penicillin ciprofloxacin combination effectively controlled bacterial growth. However penicillin streptomycin combination did not effectively control the growth. Accordingly, the combination of penicillin- ciprofloxacin seems to be a satisfactory substituent for penicillin- streptomycin combination for controlling bacterial contaminants in diluted bull semen without impairing semen characteristics.

SEASONAL VARIATIONS ON SEMEN CHARACTERISTICS OF FATTAILED AND GARUT RAMS UNDER TROPICAL CONDITIONS

This study investigated the influences of rainy and dry seasons on semen characteristics of fat-tailed and Garut rams under tropical conditions. A total of 4 healthy rams (2 fat-tailed rams and 2 Garut rams) aged 3 to 4 years old were used in this study. The semen was collected from each ram during November 2016 to February 2017 (rainy season) and May to August 2017 (dry season). The measurement of semen characteristics including semen volume (SV), sperm concentration (SpC), total sperm (TSp), individual sperm motility (ISM), post-thawing sperm motility (PTSM), and frozen semen production (FSP). The rainy season resulted higher SV (P= 0.021), TSp (P= 0.005), ISM (P= 0.028), and FSP (P= 0.005) than the dry season. On the other hand, fat-tailed rams had higher (P0.001) SV, TSp, and FSP compared to Garut rams. There was no interaction (P0.05) between season and breed on all semen characteristic parameters found in the present study. It can be concluded that the semen collection during the rainy season had a beneficial impact to produce high-quality semen for artificial insemination program than in the dry season. Fat-tailed rams have better semen characteristics compared to Garut rams.

Analisis Bobot Badan dan Karakteristik Semen Ayam Gaok Terseleksi Generasi ke-6

ABSTRAK. Ayam Gaok merupakan salah satu rumpun ayam lokal yang memiliki potensi besar dikembangkan. Balai Penelitian Ternak (Balitnak) telah melakukan program seleksi pada ayam Gaok untuk menghasilkan bibit ayam lokal pedaging yang dipanen umur 10 minggu dan dapat dijadikan galur jantan (male line) yang akan disilangkan dengan ayam KUB yang merupakan galur betina (female line). Tujuan dari penelitian ini untuk mengetahui pertumbuhan bobot badan dan karakteristik semen ayam Gaok generasi ke-6 yang dipelihara di Balitnak. Sebanyak 211 ekor anak ayam Gaok umur satu hari dari generasi ke-6 dipelihara pada kandang koloni pada masa starter dan grower. Ayam dewasa dipelihara pada kandang individu. Pakan dan air minum diberikan ad libitum. Bobot badan ayam jantan dan betina pada umur 10 minggu masing-masing 1.075,26 ± 156,55 g (KK= 14,56 %) dan 868,25 ± 109,34 g (KK= 12,59 %). Rerata bobot badan jantan dan betina sudah mulai berbeda nyata (P0,05) pada saat ayam berumur lima minggu. Konsumsi dan konversi pakan sampai umur 10 minggu yakni masing-masing 2.801,61 g dan 2,99. Bobot badan ayam jantan dan betina pada umur 20 minggu masing-masing 2.354,01 ± 280,06 (KK= 11,90%) dan 1.646,5 ± 205,97 g (KK= 12,51%). Volume semen, konsentrasi, motilitas dan persentase sperma hidup ayam Gaok jantan pada umur delapan bulan masing-masing sebesar 0,4 ml, 3.927 × 106, 57,22 dan 62,5%. Bobot badan ayam Gaok umur 10 minggu menunjukkan hasil baik dengan variasi yang cukup seragam. Kuantitas dan kualitas semen ayam Gaok tergolong normal. (Analysis of body weight and semen characteristic of 6th generation selected gaok chicken) ABSTRACT. Gaok chicken is one of local breed chicken which potentially to be developed. Indonesian Research Institute for Animal Production (IRIAP) have been executed selection program on Gaok chicken to obtain meat type local chicken which is harvested on 10 weeks old age and can be made as male line to be crossed with KUB chicken which is the female line. The objective of this study was to observe growth of body weight and characteristics of semen of Gaok chicken 6th generation which are raised in IRIAP. A number of 211 DOC Gaok chicken 6th generation were raised in colony cage in starter and grower periods. Mature chickens were kept in individual cages. Feed and water were given ad libitum. Body weight male and female at 10 weeks were 1,075.26 ±156.55 g (CV=14,56 %) and 868.25 ± 109.34 g (CV=12,59 %) respectively. Body weight on male and female were started significantly differ from five weeks (P0.05). Feed consumption and conversion for 10 weeks were 2,901.61 g and 2.99, respectively. Body weight male and female at 20 weeks were 2,354.01 ± 280.06 g (CV=11,90 %) and 1,646.5 ± 205.97 g (CV=12,51 %) respectively. Semen volume, concentration, motility and proportion of live sperm of male Gaok chicken were 0.4 ml, 3,927 × 106, 57,22 % and 62.5 %, respectively. 10th week body weight of Gaok chicken showed good results with uniform variation. Quantity and quality of Gaok chicken semen belonged to be normal.

Effects of Energy-Protein Balance in the Diet on Semen Characteristic of West Java Local Ducks

Cihateup (West Java highland) and Rambon (West Java lowland) ducks are two local ducks which are commonly raised by farmers in West Java under extensive systems. The decline of land availability as a result of increasing human population has meant that the ducks should be raised under intensive system. An experimental research was conducted to evaluate the effect of energy-protein balance in the diet on semen characteristic of Cihateup and Rambon drakes under restricted water system (intensive system). The research was designed in Completely Randomized Design; there were six energy-protein balance in the diets as treatment, which were G1 ( 3000 kcal/kg ME, 20 % CP); G2 ( 3000 kcal/kg ME, 16 % CP); G3 ( 3000 kcal/kg ME, 13.5% CP); G4 (2700 kcal/kg ME, 20 % CP); G5 (2700 kcal/kg ME, 16 % CP); and G6 ( 2700 kcal/kg ME, 13.5 % CP); each treatment was replicated three times. Semen was collected when the drakes reached the age of nine months and semen evaluation for volume semen, sperm motility and sperm abnormality was conducted. The results show that semen characteristics show that the highest motility was produced by G5 (2700 kcal/kg ME, 16 % CP) for Cihateup drakes and G6 ( 2700 kcal/kg ME, 13.5 % CP) for Rambon drakes. There was no significant different effect among treatments on abnormality of Cihateup drakes semen; anyhow G5 (2700 kcal/kg ME, 16 % CP) can be considered as the best diet for Rambon drakes based on its semen abnormality. It can be concluded that at feeding diet containing similar Metabolizable Energy level, Cihateup drakes required higher protein level than Rambon drakes to produce a good quality of semen.Keywords: energy-protein balance, west java local drakes, semen