EFFECT OF PENICILLIN -CIPROFLOXACIN COMBINATION ON SEMEN CHARACTERISTIC AND BACTERIAL CONTAMINANTS OF DILUTED BULL SEMEN

Effect of penicillin- ciprofloxacin combinations on some semen characteristics and bacterial contaminants of diluted bull semen was determined and compared with those of penicillin- streptomycin combinations. Sperm motility, percentage of dead and abnormal spermatozoa and survival index did not differ significantly for penicillin (1000 IU) and ciprofloxacin (125, 250, 500, 1000 ug) combinations per milliliter of diluted bull semen from those of penicillin (1000 I.U) and streptomycin (500 ug) combination / ml. Each penicillin ciprofloxacin combination effectively controlled bacterial growth. However penicillin streptomycin combination did not effectively control the growth. Accordingly, the combination of penicillin- ciprofloxacin seems to be a satisfactory substituent for penicillin- streptomycin combination for controlling bacterial contaminants in diluted bull semen without impairing semen characteristics.

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SEASONAL VARIATIONS ON SEMEN CHARACTERISTICS OF FATTAILED AND GARUT RAMS UNDER TROPICAL CONDITIONS

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v15i1.15168 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Nurul Isnaini ◽

Sri Wahjuningsih ◽

Moh. Miftahul Falah ◽

Alfa Fajarrofa ◽

Tri Harsi ◽

...

Keyword(s):

Sperm Motility ◽

Rainy Season ◽

Sperm Concentration ◽

Dry Season ◽

Frozen Semen ◽

Semen Volume ◽

Tropical Conditions ◽

Semen Characteristics ◽

Beneficial Impact ◽

Semen Characteristic

This study investigated the influences of rainy and dry seasons on semen characteristics of fat-tailed and Garut rams under tropical conditions. A total of 4 healthy rams (2 fat-tailed rams and 2 Garut rams) aged 3 to 4 years old were used in this study. The semen was collected from each ram during November 2016 to February 2017 (rainy season) and May to August 2017 (dry season). The measurement of semen characteristics including semen volume (SV), sperm concentration (SpC), total sperm (TSp), individual sperm motility (ISM), post-thawing sperm motility (PTSM), and frozen semen production (FSP). The rainy season resulted higher SV (P= 0.021), TSp (P= 0.005), ISM (P= 0.028), and FSP (P= 0.005) than the dry season. On the other hand, fat-tailed rams had higher (P0.001) SV, TSp, and FSP compared to Garut rams. There was no interaction (P0.05) between season and breed on all semen characteristic parameters found in the present study. It can be concluded that the semen collection during the rainy season had a beneficial impact to produce high-quality semen for artificial insemination program than in the dry season. Fat-tailed rams have better semen characteristics compared to Garut rams.

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Effect of docosahexanoic acid on quality of frozen–thawed bull semen in BioXcell extender

Reproduction Fertility and Development ◽

10.1071/rd15089 ◽

2017 ◽

Vol 29 (3) ◽

pp. 490 ◽

Cited By ~ 3

Author(s):

Asmatullah Kaka ◽

Wahid Haron ◽

Rosnina Yusoff ◽

Nurhusien Yimer ◽

A. M. Khumran ◽

...

Keyword(s):

Lipid Peroxidation ◽

Liquid Nitrogen ◽

Sperm Motility ◽

Membrane Integrity ◽

Optimum Level ◽

Docosahexanoic Acid ◽

Normal Morphology ◽

Bull Semen ◽

Acrosome Integrity

This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen–thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15 ng mL–1 DHA was added. The supplemented semen samples were incubated at 37°C for 15 min for DHA uptake by spermatozoa. Later, samples were cooled for 2 h at 5°C and packaged into 0.25-mL straws, frozen in liquid nitrogen for 24 h and subsequently thawed for evaluation. Results are presented as percentages ± s.e.m. Supplementation with DHA at 3 ng mL–1 significantly improved sperm functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane integrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3 ng mL–1 concentration of DHA resulted in superior quality of frozen–thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.

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Relationship between semen characteristics and body size in Barbus barbus L. (Teleostei: Cyprinidae) and effects of ions and osmolality on sperm motility

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpa.2009.04.001 ◽

2009 ◽

Vol 153 (4) ◽

pp. 430-437 ◽

Cited By ~ 26

Author(s):

S.M. Hadi Alavi ◽

Marek Rodina ◽

Tomáš Policar ◽

Otomar Linhart

Keyword(s):

Body Size ◽

Sperm Motility ◽

Barbus Barbus ◽

Semen Characteristics

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Effect of Supplementation of Polyvinylpyrrolidone in Extender on Buffalo Semen Parameters

Pakistan Journal of Zoology ◽

10.17582/journal.pjz/20181230161216 ◽

2020 ◽

Vol 53 (1) ◽

Author(s):

Bushra Ismail Khan ◽

Shamim Akhter ◽

Sanwal Aslam ◽

Rabea Ejaz

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Membrane Integrity ◽

Bubalus Bubalis ◽

Semen Parameters ◽

Suction Pump ◽

Bull Semen ◽

Buffalo Semen ◽

Live Sperm ◽

And Control

The current study was planned to evaluate the supplementation of Polyvinylpyrrolidone in extender on cryopreservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4°C, plunged and stored in liquid nitrogen (-196°C). Semen straws were rewarmed at 37°C for 30 seconds and assessed for sperm motility, plasma membrane integrity (PMI), dead sperm percentage and the live sperm percentage. The data on the role of PVP on different parameters of semen quality were analyzed by using ANOVA and RCBD. Higher percentage (P< 0.05) of sperm motility (66.1±7.51 and 59.4±10.72) and PMI (72.9±5.39 and 75.7±6.5) was observed in extenders having 0.05 mM and 0.1mM PVP compared to extenders having 1.5mM PVP and control. The percentage acrosomal integrity was observed greater (P< 0.05) in extended semen containing 0.1mM (68.2±0.50) PVP compared to extenders having 0.01 and control.

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The Effect of Temperature on Sperm Motility. II. Is Bacterial Growth a Factor?**Presented at the Thirty-Fourth Annual Meeting of The American Fertility Society, March 29 to April 1, 1978, New Orleans, La.

Fertility and Sterility ◽

10.1016/s0015-0282(16)43578-8 ◽

1978 ◽

Vol 30 (4) ◽

pp. 436-438 ◽

Cited By ~ 11

Author(s):

Rodney A. Appell ◽

Paul R. Evans

Keyword(s):

New Orleans ◽

Annual Meeting ◽

Sperm Motility ◽

Bacterial Growth ◽

Effect Of Temperature ◽

American Fertility Society

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76 THE EFFECT OF GLYCEROL CONCENTRATIONS IN EGG-YOLK CITRATE EXTENDER ON THE QUALITY OF CRYOPRESERVED NGUNI BULL SEMEN

Reproduction Fertility and Development ◽

10.1071/rdv25n1ab76 ◽

2013 ◽

Vol 25 (1) ◽

pp. 185

Author(s):

M. M. Seshoka ◽

M. L. Mphaphathi ◽

F. V. Ramukhithi ◽

T. R. Netshirovha ◽

C. Hlungwani ◽

...

Keyword(s):

Sperm Motility ◽

Rural Areas ◽

Egg Yolk ◽

Positive Control ◽

Negative Control ◽

Glycerol Concentration ◽

Bull Semen ◽

Frozen Semen ◽

Thawing Process ◽

Motility Rate

There are bull shortages in South African poor rural areas. Artificial-insemination technology could play a significant role on breeding emerging farmer’s cattle. The objective of this study was to compare glycerol concentrations (0, 4, 8, or 12%) during freezing of Nguni bull semen to conduct AI in different villages. Semen was collected by electro ejaculator from 2 Nguni bulls of known and proven fertility. Collected semen samples were kept in a thermo flask (37°C) and transported to the laboratories within 10 min after collections. Semen samples were pooled and evaluated by Sperm Class Analyser® and allocated randomly per treatment. Semen was then diluted (1 : 2 v:v) with egg-yolk citrate extender supplemented with either 0% (negative control), 4, 8, and 12% of glycerol concentration or AndroMed® (positive control). Semen samples were equilibrated for 4 h at 5°C. After equilibration period, samples were loaded into 0.5-mL straws and placed horizontally into the controlled rate (–5, –8, –10, –12, –15, –25, –35°C min–1) from 5°C until target temperature of –80°C is reached. The frozen semen straws were stored in a liquid nitrogen tank (–196°C) until thawing. Treatment means were separated using Fisher’s protected t-test least, and data are presented as mean ± SD. There was a significant differences (P < 0.05) between raw total sperm motility (83.3 ± 19.3) and frozen–thawed sperm with either 0% (0.0 ± 0.0), 4% (30.2 ± 13.4), 8% (47.9 ± 12.5), or 12% (61.5 ± 4.7) of glycerol and on AndroMed® (27.7 ± 17.8) group. Regardless of the glycerol concentrations used, the freezing-thawing process reduced (P < 0.05) the Nguni total sperm motility rate compared to uncryopreserved sperm (83.3 ± 19.3). In conclusion, egg-yolk citrate extender supplemented with 12% glycerol yielded a better (P < 0.05) total sperm motility rate (61.5 ± 4.7) as compared with the 0% (0.0 ± 0.0), 4% (30.2 ± 13.4), 8% (47.9 ± 12.5), and AndroMed® (27.7 ± 17.8) group. Further studies are required to test other levels of glycerol concentrations (>12%) on freezing Nguni semen and conducting AI.

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163 EVALUATION OF VIABILITY OF BULL SEMEN COLLECTED BY ELECTRO-EJACULATION USING COMMERCIAL SEMEN EXTENDER AND 2 CULTURE MEDIA AT CONTROLLED ROOM TEMPERATURE

Reproduction Fertility and Development ◽

10.1071/rdv29n1ab163 ◽

2017 ◽

Vol 29 (1) ◽

pp. 190

Author(s):

A. M. Raseona ◽

O. A. Ajao ◽

L. D. Nethengwe ◽

L. R. Madzhie ◽

T. L. Nedambale ◽

...

Keyword(s):

Sperm Motility ◽

Assisted Reproductive Technologies ◽

Culture Media ◽

Reproductive Technologies ◽

Sperm Viability ◽

Progressive Motility ◽

Bull Semen ◽

Significant Difference ◽

Semen Extender ◽

Motility Rate

Preservation of semen is an important process to ensure that semen quality is sufficient for assisted reproductive technologies. The aim of this study was to evaluate the viability of bull semen collected by electro-ejaculation using commercial semen extender and 2 modified culture media stored at controlled RT (24°C) for 72 h. Two Nguni bulls were used for semen collection; after collection, the semen was evaluated macroscopically for volume, pH, and colour, and microscopically for sperm motility, viability, and morphology. Uncontaminated semen samples with progressive motility >70% and morphological defects <20% were pooled after collection before being aliquoted into 3 extenders, namely Triladyl, modified Ham’s F10, and TCM-199 culture media, at a dilution ratio of 1:4 and then stored at controlled RT (24°C). Sperm motility rate was analysed using the computer-aided sperm analyser after 0, 24, 48, and 72 h of storage. Sperm morphology and viability was performed after staining the sperm cells with spermac and nigrosine-eosin stain, respectively. The study was replicated 4 times and data were analysed using ANOVA. Triladyl had a higher sperm viability rate (41.3%) and total motility rate (96.3%) for 72 h (P < 0.01) compared with the 2 modified culture media, Ham’s F10 (26.5 and 86.8%) and TCM-199 (25.0 and 86.7%), respectively. However, Ham’s F10 had higher progressive motility rate (37.8%) as compared with the other extenders, TCM-199 (31.7%) and Triladyl (23.4). There was no significant difference (P > 0.05), in viability rate between Ham’s F10 (26.5%) and TCM-199 (25.0%). No significant difference (P > 0.05) in straight line velocity was observed for the three extenders. Furthermore, no significant difference was observed in total sperm abnormalities, except for reacted acrosomes and absent tails (P > 0.05), between the 2 Nguni bulls. Nguni semen can be preserved in Triladyl or modified Ham’s F10 and TCM-199 culture media stored at 24°C and stay viable for 72 h. Triladyl proved to be the best suitable extender of the 3 extenders, showing higher sperm viability and total motility rate as compared with Ham’s F10 and TCM-199 modified culture media.

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Chlorhexidine Bathing and Microbial Contamination in Patients’ Bath Basins

American Journal of Critical Care ◽

10.4037/ajcc2012242 ◽

2012 ◽

Vol 21 (5) ◽

pp. 338-342 ◽

Cited By ~ 12

Author(s):

Jan Powers ◽

Jennifer Peed ◽

Lindsey Burns ◽

Mary Ziemba-Davis

Keyword(s):

Bacterial Growth ◽

Microbial Contamination ◽

Chlorhexidine Gluconate ◽

Coagulase Negative Staphylococcus ◽

Fisher Exact Test ◽

Exact Test ◽

Hospital Acquired Infections ◽

Bacterial Contaminants ◽

Gram Negative Organisms ◽

Hospital Acquired

Background Research has demonstrated the hazards associated with patients’ bath basins and microbial contamination. In a previous study, soap and water bath basins in 3 acute care hospitals were found to be reservoirs for bacteria and potentially associated with the development of hospital-acquired infections. Bacteria grew in 98% of the basin samples; the most common were enterococci (54%), and 32% were gram-negative organisms. Objective To assess the presence of bacterial contaminants in wash basins when chlorhexidine gluconate solution is used in place of standard soap and water to wash patients. Methods Bathing with chlorhexidine gluconate is the standard of practice for all patients in intensive care units at St Vincent Hospital. Specimens from 90 bath basins used for 5 days or more were cultured for bacterial growth to assess contamination of basins when chlorhexidine gluconate is used. Results Of the 90 basins cultured, only 4 came back positive for microbial growth; all 4 showed growth of gram-positive organisms. Three of the 4 organisms were identified as coagulase-negative staphylococcus, which is frequently found on the skin. This translates into a 95.5% reduction in bacterial growth when chlorhexidine gluconate is used as compared with soap and water in the previous study (Fisher exact test, P < .001). The only factor that was related to positive cultures of samples from basins was the sex of the patient. Discussion Compared with the previous study examining microbial contamination of basins when soap and water was used to bathe patients, bacterial growth in patients’ bath basins decreased significantly with the use of chlorhexidine gluconate, drastically reducing the risk for hospital-acquired infections. Such reduced risk is especially important for critically ill patients at high risk for bacterial infection.

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