Congenital amusia is a neurogenetic disorder of pitch perception that may also compromise pitch production. We explored whether vocal imitation of song in amusia is influenced by melody familiarity and phonetic content. Thirteen Mandarin-speaking amusics and 13 matched controls imitated novel song segments with lyrics and on the syllable /la/ while their output was recorded. Eleven out of these participants in each group also imitated segments of a familiar song. Subsequent acoustic analysis was conducted to measure pitch and timing matching accuracy. While amusics’ performance was facilitated by melody familiarity in terms of pitch interval deviation, signed interval deviation and number of contour errors, they showed compromised performance compared to controls in absolute pitch deviation, number of pitch interval errors, duration difference, interonset interval difference, and number of time errors in both familiar and novel song conditions. The presence of lyrics did not affect either group’s performance substantially. While a stronger relationship between music perception and novel melody imitation was observed in amusics as opposed to familiar melody imitation, controls showed the opposite pattern. We discuss the implications of the study in terms of music familiarity, memory demands, the relevance of lexical information and the link between perception and production.