Comparative analysis of phenolic content in Solanum indicum L. harvested from different locations of Madhya Pradesh state of India

Phenolics are well distributed secondary metabolites in the plant kingdom and cure various ailments in human beings. In the present study, total phenolic (TP) and caffeic acid (CA) contents in fruits, leaves and stem of Solanum indicum species collected from different locations of Madhya Pradesh state of India were studied. Results showed the following trend for TP and CA contents; fruits> leaves> stem. Fruits (28.52±0.29) from Chhindwara & Betul area (Satpura plateau agroclimatic region) contained maximum TP content trailed by leaves (16.29±0.07) and stem (11.79±0.03) belonging to Amarkantak area (Northern Hill’s Zone of Chhattisgarh agroclimatic region). CA content was observed maximum in fruits (0.0192±0.00) followed by leaves (0.0187±0.01) and stem (0.0154±0.01) of Seoni area (Kymore Plateau & Satpura Hills agroclimatic region). The variation in the populations will be helpful for in-situ as well as ex-situ conservation of this reagionally threatened dashmool species and its further utilization in Ayurvedic formulations.

Evaluation of phenolic, flavonoid contents and antioxidant activity in leaves, stem and roots of Solanum indicum Linn.

The present investigation deals with the evaluation of phenolic, flavonoid contents and antioxidant activity in leaves, stem and roots of Solanum indicum. Powdered samples of leaves, stem and roots were subjected to successive extraction with solvents of increasing polarities i.e. Ethanol, Water: Ethanol (Aqua- alcoholic) (20: 80) and Water (aqueous) using soxhlet apparatus. The results showed the maximum phenolic content (12.351±0.03%) in Aqua- alcoholic (80: 20) extract and maximum flavonoid content (38.658±0.05%) in ethanolic extract of leaves. For stem, both the maximum phenolic (11.750±0.02%) and flavonoid (2.040±0.03%) contents was found in Aqua- alcoholic (80: 20) extract. Similarly, the maximum phenolic (18.651±0.03%) and flavonoid (2.701±0.05%) contents was found in ethanolic extract of roots. The Aqua- alcoholic (80: 20) extracts for leaves and stem and ethanolic extract for roots was found to contain the maximum antioxidant activity.

Validated Chromatographic methods for estimation of Solanine in Mahamrutyunjaya rasa

Introduction: Mahamrutyunjaya rasa is a herbomineral ayurvedic formulation used for various indications like fever, pain, cardiac arrhythmia, etc. It contains Solanum indicum as one of the ingredients having anti-inflammatory, diuretic, and diaphoretic properties. Solanine is one of the active biomarkers for Solanum indicum. The therapeutic window of Solanine is very narrow, with the LD50 value of 3-6 mg/kg body weight in humans. Objective: Estimation of such biomarkers is very significant to avoid any adverse events due to the administration of ayurvedic preparations containing Solanine. In the present study, two simple, sensitive, reliable chromatographic techniques have been developed and validated for the estimation of Solanine in Mahamrutyunjaya rasa. Materials and Methods: High-performance liquid chromatography (HPLC) separation of Solanine was performed on an reversed phase C-18 column (250 mm 9 4.6 mm ID, 5 lm particle size), with isocratic elution using a mixture of Tris buffer (10mM, pH 6.00): Acetonitrile(60:40, v/v) at a flow rate of 1 mL/min with UV detection at 218 nm for solanine. High-performance thin-layer chromatography (HPTLC) separation was done on Silica gel 60 F254 pre-coated plates using Chloroform: Methanol: Ammonia (7:3:0.5 v/v). The densitometric scanning was performed at 500 nm. The developed methods were validated for linearity, LOD, LOQ, accuracy, precision, and specificity as per ICH guidelines. Results: Solanine was eluted at 4.43 ± 0.1 min and established a linearity range of 1 – 100 μg/ml (r2 = 0.9992). In the HPTLC method, the Rf value of Solanine was 0.05 in a linearity range of 1600-4800 ng/ml. Conclusion: Reliable, rapid, simple, and sensitive chromatographic methods were developed for the quantification of Solanine in Mahamrutyunjaya Rasa.

Identifikasi Tumbuhan Famili Solanaceae Yang Terdapat Di Kecamatan Tugumulyo

Tujuan dari penelitian ini adalah untuk mengetahui jenis-jenis tumbuhan famili Solanaceae di Kecamatan Tugumulyo. Adapun jenis penelitian ini adalah deskriptif eksplorasi. Teknik pengambilan data dengan observasi dan wawancara. Cara kerja yang dilakukan yaitu observasi lapangan dan observasi laboratorium. Data yang diperoleh dari penelitian dianalisis secara deskriptif kualitatif, yaitu dengan mencocokkan ciri-ciri sampel yang telah ditemukan dengan buku acuan yaitu buku karangan Gembong Tjitrosoepomo tahun 2010 dan Van Steenis., et altahun 2008. Hasil penelitian didapat 5 genus dan 11 species yaitu Genus Solanum diperoleh 6 species: Solanum indicum (Terung peuheur), Solanum lycopersicum (Tomat), Solanum melongena (Terung), Solanum nigrum (Leunca)dan Solanum torvum (Takokak), Solanum tuberosum(Kentang). Kemudian genus Capsicum diperoleh 2 species:Capsicum annum(Cabe merah)danCapsicum frutescens (Cabe rawit), genus Datura diperoleh 1 species: Datura metel (Kecubung), genus Brugmansia diperoleh 1 species: Cestrum nocturnum (Harum Dalu), dan genus Physalis diperoleh 1 species:Physalis angulata(Ceplukan).

Evaluation of Acute and Sub-acute Toxicity of the Aqueous Extract from the Fruit of Solanum indicum Linn. (Solanaceae) in Rats

Aim: The fruit of Solanum indicum Linn have been reported traditionally to have anti-hypertensive and diuretic properties. This study was undertaken to evaluate the toxicological potential of S. indicum fruits aqueous extract through the acute and sub-acute toxicity tests in rats. Methodology: For acute toxicity evaluation, a single oral dose of 5000 mg/kg of the plant extract was administrated in 60 days old female albino Wistar rats. Then, the animals were observed for 14 days. Sub-acute toxicity studies were conducted with 50 adult rats of both gender that orally received during 28 days, increasing doses of the plant extract. Their body weight and food intake were weekly collected. At the end of the experiment, biochemical and hematological parameters as well as histological analysis of organs (liver, kidneys and spleen) were undertaken. Results: Single oral administration of 5000 mg/kg dose of the fruit plant aqueous extract produced no mortality or signs of toxicity. During sub-acute test, no variations in body weight and food intake of both animals gender were observed. An important decrease in male’s rat liver weight were obtained at the dose 25 mg/kg; serum urea, total cholesterol, TAG, ALP and AST levels were significantly lowered in male especially at the dose 50 mg/kg, but this decrease was noticed only in serum urea, ALP and ALT in female rats. Furthermore, a significant decrease in platelets number, serum PCT, MPV and PDW levels were recorded in all treated male rats except those receiving the highest extract dose. No structural changes in treated animal organs section histology were observed when compared to controls. Conclusion: The fruits aqueous extracts of S. indicum is safe when administered acutely and for 28 days in rats. However, alterations on their hematological and biochemical parameters were not closely related with the dose, implying caution on its use.