A Preliminary HPTLC fingerprint study of Madhuka Indica –An Immune Enhancer

– Ayurveda is now accepted as a global medical science. The world is shifting to herbal remedies which is considered to be safe and effective. But the quality maintenance of these herbal medicines is a difficult task which hinders the globalization of Ayurvedic drugs and thus it’s a need to use suitable techniques to maintain relevant standards. Standardization is very much important for the reproducibility of the efficacy and safety of any drug which is brought to the counter as medicine for sale. Madhuka (1)has been mentioned in classical texts as brimhana, shukrakruta , veerya pushtivardhana, dahahara, shramahara, vatapittavinasham, bhutadi jantudoshagna ,kshatkshayharam etc. This article aims to explore the qualities of Madhuka pushpa, its phytochemical parameters with HPTLC and the preliminary standardization of preparing madhuka pushpa vati for its use as an immune enhancer

Recombinant Avian β-Defensin Produced by Food-Grade Lactococcus as a Novel and Potent Immunological Enhancer Adjuvant for Avian Vaccine

In this study, we expressed rAvBD1-2–6-13 protein through Lactococcus lactis NZ3900, and the effects of the recombinant L. lactis NZ3900 as an immune enhancer and immune adjuvant were verified using in vivo and in vitro tests. In vitro tests revealed that recombinant L. lactis NZ3900 significantly activated the NF-κB signaling pathway and IRF signaling pathway in J774-Dual™ report cells and significantly increased the transcript levels of IL-10, IL-12p70, CD80, and CD86 in chicken PBMCs and chicken HD11 cells. In vivo experiments revealed that the immunized group supplemented with recombinant L. lactis NZ3900 as an adjuvant had significantly higher serum antibody titers and higher proliferative activity of PBMCs in the blood of the chickens immunized with NDV live and inactivated vaccines. Our study shows that the recombinant L. lactis NZ3900 has strong immunomodulatory activity both in vivo and in vitro and is a potential immune enhancer. Our work lays the foundation for the research and development of new animal immune enhancers for application in the poultry industry.

Pengaruh Pemberian Ekstrak Stichopus hermanii Semper, 1868 (Stichopodidae, Holothuroidea) terhadap Jumlah Total Hemosit Litopenaeus vannamei Boone, 1931 (Penaeidae, Crustacea)

Peningkatan imunitas udang vaname dengan menggunakan imnostimulator merupakan salah satu upaya untuk mencegah kegagalan panen pada budidaya udang vaname. Ekstrak teripang emas memiliki senyawa yang berperan sebagai peningkat imun. Beberapa senyawa yang terkandung pada teripang emas yaitu saponin dan steroid. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak teripang emas terhadap jumlah total hemosit Litopenaeus vannamei dan konsentrasi ekstrak teripang emas yang paling tepat untuk meningkatkan jumlah total hemosit udang vaname. Metode yang digunakan adalah metode eksperimen laboratoris dengan perlakuan konsentrasi ekstrak teripang emas yang ditambahkann pada pakan udang komersil yaitu 0 ppm; 40 ppm; 80 ppm; 120 ppm. Hasil penelitian jumlah total hemosit udang vaname terjadi peningkatan akibat pemberian ekstrak teripang emas dibandingkan kontrol. Peningkatan tersebut terlihat pada jumlah total hemosit udang vaname yang diberi ekstrak teripang emas dengan konsentrasi 40 dan 120 ppm pada hari ke-8. Pemberian ekstrak teripang emas dengan konsentrasi 120 ppm memberikan hasil terbaik yaitu jumlah total hemosit sebanyak 1,18x106 sel/mL, dan untuk kelangsungan hidup udang vaname yang diberikan ekstrak teripang emas dan kontrol memiliki persentase sebesar 100%. Kesimpulannya adalah pemberian ekstrak teripang emas berpengaruh terhadap jumlah hemosit udang vaname. Increased immunity of vaname shrimp by using imnostimulator is one of the efforts to prevent crop failure in the cultivation of vaname shrimp. gold sea cucumber extract has a compound that acts as an immune enhancer. Some of the compounds contained in Stichopus hermanii are saponins and steroids. The purpose of this study was to determine the effect of gold sea cucumber extract on the total amount of vaname shrimp haemocyte and the most appropriate concentration of golden sea cucumber extract to increase the total amount of vaname shrimp haemocyte. The method used was a method of laboratory experimentation with the treatment of the concentration of Stichopus hermanii extract added to commercial shrimp feed that is 0 ppm; 40 ppm; 80 ppm; 120 ppm. The results of the study the total amount of shrimp haemocyte Litopenaeus vananamei increased due to administration of Stichopus hermanii extract compared to control. The increase was seen in the total amount of vaname shrimp haemocyte given gold sea cucumber extract with concentrations of 40 and 120 ppm on day 8. Administration of gold sea cucumber extract with a concentration of 120 ppm gives the best result that is the total amount of haemocyte as much as 1.18x106 cells / mL, and for the survival of Litopenaeus vannamei given extract gold sea cucumber and control has a percentage of 100%. The conclusion is that the administration of gold sea cucumber extract affects the amount of haemocyte shrimp vaname.

PERBANDINGAN EKSTRAKSI JAMUR TIRAM (Pleurotus ostreatus) DAN JAMUR KANCING (Agaricus bisporus)

Jamur mengandung polisakarida dengan komponen utama yaitu β-glucan dan αmannan. Polisakarida dapat bermanfaat sebagai immune enhancer karena β-glukan dapat meningkatkan aktivitas fagositosis dan produksi sitokin. β-(1,3)-glukan memiliki kemampuan mengaktivasi sistem imunitas seluler dan humoral yang merupakan komponen dari sistem imunitas tubuh. β-(1,3)-glukan meningkatkan aktivitas antimikroba dari sel mononuklear dan neutrofil dan meningkatkan aktivitas fungsional dari makrofag. Polisakarida dari jamur tiram dan jamur kancing dapat diekstraksi menggunakan metode Yap & Ng. Tujuan penelitian ini adalah membandingkan rendemen ekstrak yang dihasilkan dari jamur tiram dan jamur kancing. Metode yang digunakan dalam penelitian ini adalah metode ekstraksi air berdasarkan Yap & Ng. Analisis data penelitian ini dilakukan dengan menghitung persentase rendemen ekstrak dari jamur tiram dan jamur kancing, kemudian dibandingkan. Hasil penelitian ini menunjukkan bahwa rendemen ekstrak jamur tiram sebesar 0,013% sedangkan rendemen ekstrak jamur kancing sebesar 0,0055%. Hal ini menunjukkan rendemen ekstrak jamur tiram lebih besar daripada rendemen ekstrak jamur kancing jika diekstraksi dengan metode Yap & Ng.