Deteksi Level Antibodi Pada Serum Darah Anjing Kintamani Pasca Vaksinasi Rabies dengan Direct ELISA

The success of rabies vaccination is characterized by the growth of seropositive antibody titers (?0.5 IU) after vaccination. One of the tests conducted to monitor antibody growth is ELISA (Enzyme Linked Immunosorbent Assay). This study aimed to determine the effect of different vaccine doses on the growth of rabies antibodies in kintamani dogs. The study was conducted using an experimental method with a study design using 2 variables, namely the difference in vaccine doses (0.5 and 0.75 cc) and the times of blood collection (1, 2 and 3 months after vaccination). Each vaccine dose was given to three kintamani dogs. Antibodies were measured using ELISA and data were analyzed with paired sample t-test between the treatment dose of 0.75 cc and 0.5 cc at months 1.2 and 3 after vaccination. The results showed that at first month the antibody titer were 2,12 IU at a dose of 0.75 cc and 3.72 IU at a dose of 0.5 cc. At second months, antibody titers were 7.74 IU (0.75 cc dose) and 10.85 IU (0.5 cc dose), while at third month, antibody titers were 5.73 IU (0.75 cc) and 9.00 IU (0.5 cc). Vaccine doses administered produce antibody titer levels that did not differ significantly between 0.75 cc and 0.5 cc doses. Keyword: Antibody, ELISA, Kintamani Dog, Rabies

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Changes in Spike Protein Antibody Titer Over 90 Days after the Second Dose of SARS- CoV-2 Vaccine in Japanese Dialysis Patients

10.21203/rs.3.rs-1165368/v1 ◽

2021 ◽

Author(s):

Haruki Wakai ◽

Natsumi Abe ◽

Touno Tokuda ◽

Rika Yamanaka ◽

Satoshi Ebihara ◽

...

Keyword(s):

Antibody Titer ◽

Messenger Rna ◽

Vaccine Dose ◽

Spike Protein ◽

Dialysis Patients ◽

Protein Levels ◽

The Status ◽

Antibody Titers ◽

Baseline Characteristics ◽

Spread Of Infection

Abstract Objectives There is no report on antibody titers after vaccination against SARS-CoV-2 in Japanese dialysis patients. As dialysis is different between Japan and other countries, changes in antibody titers were examined. Methods Baseline characteristics and anti-spike protein antibody titers (Roche) over 90 days after administration of the BNT162b2 messenger RNA vaccine were investigated in dialysis patients. Results The maximum anti-spike protein antibody titer after the second dose was 737.9 (326.8 to 1143.4) and was reached at 19 (17 to 24.3) days after the second dose. Antibody titers decreased over time, with titers of 770 (316.4 to 1089) at 15 days, 385 (203 to 689.7) at 30 days, 253.5 (138 to 423) at 60 days, and 208 (107 to 375) at 90 days after the second dose. When an antibody titer of 137.052 U/mL was assumed to be a measure related to breakthrough infection, the proportion of subjects with antibody titers exceeding this level was 90.1％ at 15 days, 85.3% at 30 days, 75.0% at 60 days, and 65.4% at 90 days after the second dose. When a decrease in antibody titers below the assumed breakthrough level was defined as an event, subjects with a pre-dialysis albumin ≥ 3.5 were significantly less likely to experience an event than subjects with a pre-dialysis albumin < 3.5. In addition, this study suggests that the presence of anti-spike protein levels ≥ 313 at 30 days after the second vaccine dose might be a factor in maintaining antibody titers exceeding the assumed breakthrough level at 90 days after the second dose. Conclusions The maximum antibody titers and the antibody titers at 90 days after the second vaccine dose in Japanese dialysis patients were approximately 30% of those in non-dialysis individuals reported otherwise, it was similar to those in dialysis patients in other countries. Whether an additional vaccine dose is needed should be determined based on indicators serving as factors in maintaining antibody titers as well as the status of the spread of infection.

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An enzyme-linked immunosorbent assay for platelet compatibility testing

Blood ◽

10.1182/blood.v62.4.744.744 ◽

1983 ◽

Vol 62 (4) ◽

pp. 744-749 ◽

Cited By ~ 5

Author(s):

JD Tamerius ◽

JG Curd ◽

P Tani ◽

R McMillan

Keyword(s):

Antibody Titer ◽

Immunosorbent Assay ◽

Clinical Laboratory ◽

Clinical Problem ◽

Enzyme Linked Immunosorbent Assay ◽

Microtiter Plates ◽

Volunteer Donor ◽

Antibody Titers ◽

Test Serum ◽

Selection Of

Abstract The selection of platelet donors for patients who are refractory to random donor platelets often presents a difficult clinical problem. We describe an enzyme-linked immunosorbent assay (ELISA) for evaluating alloantibodies in refractory patients. Platelets from prospective donors are immobilized on microtiter plates and, after incubation with test serum and washing, platelet-bound IgG is detected with enzyme- linked anti-human IgG. Platelets from 46 prospective donors were tested. Twenty-two were judged compatible (reciprocal of the antibody titer less than 16) and, of these, 15 were used as platelet donors; each gave a measurable platelet increment after transfusion. The magnitude of the response was roughly proportional to the assay results. Platelets from donors giving antibody titers less than 4 resulted in platelet increments at 1 hr ranging from 4,890 to 22,200 (median 12,600), while platelets from donors giving titers of 8 or 16 resulted in lesser increments (550–4548). Conversely, 5 of the 24 patients found incompatible by the assay (titer greater than 16) gave no platelet increment, and in 3 instances, the recipient developed fever and chills after the transfusion. The assay is sensitive, simple, and adaptable to the clinical laboratory. Platelets from volunteer donor panels can be plated and stored for up to 6 mo.

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Production and Characterization of Antibodies Against Neosaxitoxin

Journal of AOAC International ◽

10.1093/jaoac/75.2.341 ◽

1992 ◽

Vol 75 (2) ◽

pp. 341-345 ◽

Cited By ~ 11

Author(s):

Fun S Chu ◽

Xuan Huang ◽

Sherwood Hall

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Indirect Elisa ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

High Antibody ◽

Antibody Titers ◽

Antibody Elisa

Abstract Antibody against neosaxitoxin (neo-STX) was obtained from rabbits after immunization with neo- STX conjugated to either keyhole limpet hemocyanln (KLH) or bovine serum albumin (BSA). An indirect enzyme-linked Immunosorbent assay (ELISA), In which either neo-STX-BSA or neo-STXKLH was coated to the mlcroplate, was used to monitor the antibody titer. Although high antibody titers were obtained from rabbits after immunization with both immunogens, only antibody obtained from rabbits immunized with neo-STX-KLH was useful for Immunoassay. Competitive indirect ELISA revealed that the antibodies obtained from rabbits Immunized with neo-STX-KLH are specific for neo-STX but also have good cross-reactivity with STX. The concentrations causing 50% inhibition binding of neo-STX-BSA to the anti-neo-KLH by neo-STX, STX, and decarbamoyl-STX (DC-STX) were 0.9,8.0, and 53.1 ng/mL, respectively. Saxitoxin conjugated to polylyslne (STX-PLL) was also used as the coating reagent In the indirect ELISA. The concentrations causing 50% inhibition binding of antl-neo-STX-KLH to STX-PLL coated on the mlcrotiter plate by neo-STX, STX, and DC-STX were 1.2,4.1, and 36.1 ng/mL, respectively. With this newly developed antibody, ELISA could be a very effective method for monitoring seafood for both neo-STX and STX.

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Post Vaccination Titers of Serum Anti-Diphtheria in Indonesian Young and Middle-Aged Adults

10.20944/preprints202107.0245.v1 ◽

2021 ◽

Author(s):

Gatot Soegiarto ◽

Diksi Novritasari ◽

Ari Baskoro ◽

Deasy Fetarayani ◽

Wita Kartika Nurani

Keyword(s):

Antibody Titer ◽

Serum Antibody ◽

Age Groups ◽

Middle Aged ◽

Adult Age ◽

Significant Difference ◽

Antibody Titers ◽

The Difference ◽

Middle Aged Adult ◽

Middle Aged Adults

Objective: This study aimed to evaluate the antibody responses in two adult age groups after diphtheria vaccination. Study Design: An observational analytic study was carried out to determine the difference in serum titer of anti-diphtheria antibody. Methods: Serum antibody titers were measured just before and 3 months after injection of Diphtheria toxoid vaccine. Vaccine was given to two adult age groups of health care personnel in hospital: the young (< 40 years) and the middle-aged (≥ 40 years). Data were analyzed using the Mann-Whitney test (p < 0.05). Results: Significant increase in serum anti-diphtheria antibody titers were recorded after vaccination in both age group (p < 0.001 in young adult and p = 0.001 in middle-aged adult, respectively). There were no substantial differences between the two groups in terms of antibody titer before vaccination (p = 0.741), 3 months after vaccination (p = 0.317) and in the increase of antibody titer (p = 0.479). Conclusions: This study showed that there was no significant difference in the increase of anti-diphtheria antibody titers between the two age groups, proving that both young and middle-aged adults had an equal immune response to a given diphtheria vaccine.

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Characteristics of convalescent plasma donors and their antibody titers in Bangladesh

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20211044 ◽

2021 ◽

Vol 8 (4) ◽

pp. 480

Author(s):

Ponkaj K. Datta ◽

M. Mujibur Rahman ◽

Ahmedul Kabir ◽

M. Mazharul Hoque ◽

Motlabur Rahman ◽

...

Keyword(s):

Antibody Titer ◽

Enzyme Linked Immunosorbent Assay ◽

Rt Pcr ◽

Igg Antibody ◽

Antibody Titers ◽

Qualitative Test ◽

The Mean ◽

Randomized Control ◽

Donor Criteria ◽

Donor Plasma

Background: Convalescent plasma is considered a promising therapy for severe COVID-19 disease. It is collected from the voluntary donors. Measurement of the antibody titer is necessary before transfusion to predict the outcome in the recipients. Characteristics of the convalescent plasma donors in Bangladesh and their antibody titers are not known.Methods: Convalescent plasma was collected from the voluntary donors who survived the COVID-19 disease to transfuse to the severe COVID-19 patients under a randomized control trial. Total IgG antibody titer was measured in the donor plasma by indirect enzyme-linked immunosorbent assay. Data was collected in a preformed questionnaire before donor plasma collection.Results: The median age is 32 (18-55) years. Fever, cough, sore throat, diarrhea was most common among 68.3% of the symptomatic participants and the remaining 31.7% were asymptomatic at the time when they were RT-PCR positive. Overall, 57.1% of participants had mild symptoms, 11.1% had moderate symptoms and none had severe symptoms. Participants’ antibody titers were measured 41.68±14.072SD days after the RT-PCR positive date. Rapid qualitative test could not detect antibody in 11 (17.5%) potential donors. Of the remaining 52 (82.5%) antibody positive participants titer was measured in 43 participants and found 1:320 in 17 (27.0%) (n=63), 1:160 in another 17 (27.0%) (n=63) and 1:80 in rest of the 9 (14.28%) (n=63) Participants. The mean titer of the donors who were hospitalized during their illness (1:274.29) was statistically significantly higher (p=0.043, CI>95%). The mean titer was also higher in female than in male, symptomatic than asymptomatic participants and in donors of A positive blood group. However, these finding are not statistically significant. Antibody titer does not correlate with time of RT-PCR negativity from initial RT-PCR positivity, time from RT-PCR positivity to titer date, age and body mass index.Conclusions: All RT-PCR positive COVID-19 patients subsequently may not develop antibody. Although antibody titer among hospitalized symptomatic patients was significantly higher, further study is needed to recommend optimal convalescent plasma donor criteria.

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Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations

Animals ◽

10.3390/ani11102851 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2851

Author(s):

Zineb EL Brini ◽

Ouafaa Fassi Fihri ◽

Romain Paillot ◽

Chafiqa Lotfi ◽

Farid Amraoui ◽

...

Keyword(s):

Antibody Titer ◽

Neutralization Test ◽

Enzyme Linked Immunosorbent Assay ◽

Equine Herpesvirus ◽

Specific Enzyme ◽

Blood Samples ◽

Equine Herpesvirus 1 ◽

Antibody Titers ◽

Herpesvirus 1 ◽

Herpesvirus 4

This study reports the first equine herpesvirus-1 (EHV-1) and equine herpesvirus-4 (EHV-4) seroprevalence investigation in horse populations of Morocco in 24 years. It also aims to determine antibody titers in horses vaccinated under field conditions with a monovalent EHV-1 vaccine. Blood samples were collected from 405 horses, including 163 unvaccinated and 242 vaccinated animals. They were tested using a commercial type-specific enzyme-linked immunosorbent assay (ELISA) and a virus neutralization test (VNT). Overall, 12.8% unvaccinated, and 21.8% vaccinated horses were positive for EHV-1. All samples were positive for EHV-4 when tested with the type-specific ELISA. In the vaccinated group, the VNT revealed a mean antibody titer of 1:49 for EHV-1 and 1:45 for EHV-4.

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Search for Correlates of Protective Immunity Conferred by Anthrax Vaccine

Infection and Immunity ◽

10.1128/iai.69.5.2888-2893.2001 ◽

2001 ◽

Vol 69 (5) ◽

pp. 2888-2893 ◽

Cited By ~ 175

Author(s):

Shaul Reuveny ◽

Moshe D. White ◽

Yaakov Y. Adar ◽

Yaron Kafri ◽

Zeev Altboum ◽

...

Keyword(s):

Antibody Titer ◽

Neutralizing Antibodies ◽

Protective Immunity ◽

Protective Antigen ◽

Surrogate Marker ◽

Neutralizing Antibody ◽

Enzyme Linked Immunosorbent Assay ◽

Lethal Challenge ◽

Anthrax Spores ◽

Antibody Titers

ABSTRACT Vaccination by anthrax protective antigen (PA)-based vaccines requires multiple immunization, underlying the need to develop more efficacious vaccines or alternative vaccination regimens. In spite of the vast use of PA-based vaccines, the definition of a marker for protective immunity is still lacking. Here we describe studies designed to help define such markers. To this end we have immunized guinea pigs by different methods and monitored the immune response and the corresponding extent of protection against a lethal challenge with anthrax spores. Active immunization was performed by a single injection using one of two methods: (i) vaccination with decreasing amounts of PA and (ii) vaccination with constant amounts of PA that had been thermally inactivated for increasing periods. In both studies a direct correlation between survival and neutralizing-antibody titer was found (r 2 = 0.92 and 0.95, respectively). Most significantly, in the two protocols a similar neutralizing-antibody titer range provided 50% protection. Furthermore, in a complementary study involving passive transfer of PA hyperimmune sera to naive animals, a similar correlation between neutralizing-antibody titers and protection was found. In all three immunization studies, neutralization titers of at least 300 were sufficient to confer protection against a dose of 40 50% lethal doses (LD50) of virulent anthrax spores of the Vollum strain. Such consistency in the correlation of protective immunity with anti-PA antibody titers was not observed for antibody titers determined by an enzyme-linked immunosorbent assay. Taken together, these results clearly demonstrate that neutralizing antibodies to PA constitute a major component of the protective immunity against anthrax and suggest that this parameter could be used as a surrogate marker for protection.

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Enzyme-Linked Immunosorbent Assay for Recombinant K39 Antigen in Diagnosis and Prognosis of Indian Visceral Leishmaniasis

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.8.6.1220-1224.2001 ◽

2001 ◽

Vol 8 (6) ◽

pp. 1220-1224 ◽

Cited By ~ 45

Author(s):

R. Kumar ◽

K. Pai ◽

K. Pathak ◽

S. Sundar

Keyword(s):

Visceral Leishmaniasis ◽

Antibody Titer ◽

Immunosorbent Assay ◽

Response To Therapy ◽

Enzyme Linked Immunosorbent Assay ◽

Soluble Antigen ◽

Diagnosis And Prognosis ◽

Diagnostic Potential ◽

Amino Acid Repeats ◽

Antibody Titers

ABSTRACT The recombinant product (rK39) of the 39-amino-acid repeats encoded by a kinesin-like protein-encoding gene of Leishmania chagasi was evaluated by enzyme-linked immunosorbent assay (ELISA) for diagnostic potential and the ability to predict the response to therapy in Indian kala-azar or visceral leishmaniasis (VL); we also compared its performance with that of crude soluble antigen (CSA). At the diagnosis of VL, the anti-rK39 antibody titer was 59-fold higher than the anti-CSA antibody titer. With successful therapy, antibody titers declined steeply at the end of treatment and during follow-up. In contrast, patients who relapsed showed increased titers of antibodies to rK39. The extremely high levels of anti-rK39 antibodies in VL cases suggest the application of rK39 for sensitive and specific serodiagnosis, and rK39 ELISA is also valuable in monitoring drug therapy and detecting relapse of the disease.

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EVALUASI TITER ANTIBODI PASCA VAKSINASI Septicaemia epizootica PADA SAPI BALI DI KOTA KUPANG

JURNAL KAJIAN VETERINER ◽

10.35508/jkv.v8i1.2292 ◽

2020 ◽

Vol 8 (1) ◽

pp. 69-80

Author(s):

Mario H.Cantona ◽

Maxs Urias Ebenheizer Sanam ◽

Tri Utami ◽

Tarsisius Considus Tophianong ◽

Antin Y.N Widi

Keyword(s):

Antibody Titer ◽

Enzyme Linked Immunosorbent Assay ◽

Test Results ◽

Serum Samples ◽

Average Value ◽

Antibody Titers ◽

Random Manner ◽

Bali Cattle ◽

Effect Of Age ◽

Selection Of

Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.

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Association between anti-complement factor H antibodies and renal outcome in primary membranous nephropathy

10.21203/rs.3.rs-44059/v1 ◽

2020 ◽

Author(s):

Yu Kagaya ◽

Norifumi Hayashi ◽

Keiji Fujimoto ◽

Hiroki Adachi ◽

Kengo Furuichi ◽

...

Keyword(s):

Renal Dysfunction ◽

Antibody Titer ◽

Membranous Nephropathy ◽

Factor H ◽

Complement Factor H ◽

Renal Outcome ◽

Enzyme Linked Immunosorbent Assay ◽

Complement Factor ◽

Antibody Titers ◽

Normal Controls

Abstract Background: The complement factor H (CFH) regulates activation of the alternative complement pathway. Autoantibodies against CFH are involved in progressive renal dysfunction in cases with primary membranous nephropathy (MN). However, the prevalence and roles of anti-CFH antibodies in the clinical outcome of MN patients remain unclear.Methods: We retrospectively investigated data of 36 Japanese patients with primary MN (23 men, 13 women; mean age: 64.5 [59-72] years) and 18 healthy normal controls (8 men, 10 women; mean age: 31 [27-38] years). Serum anti-CFH antibody titers were measured by enzyme-linked immunosorbent assay.Results: Anti-CFH antibody titers were significantly higher in MN patients than in normal controls (4.69 [3.69-6.38] AU/mL vs. 0 [0-0] AU/mL, p<0.001). The patients were divided into groups: anti-CFH antibody positive group (n=28) and anti-CFH antibody negative group (n=8). No significant difference was observed in the remission rate of proteinuria and the incidence of 30% reduction of estimated glomerular filtration rate (eGFR) or 50% elevation of serum creatinine (Cr) levels between both groups. Anti-CFH antibody titer was selected as an independent unfavorable predictor of renal dysfunction by Cox proportional hazards analysis adjusted by age, gender, serum Cr levels, proteinuria (g/gCr), anti-CFH antibody titer, and immunosuppressive therapy (adjusted hazard ratio (HR) 1.344, 95% confidence intervals (CI) 1.038 to 1.741, p=0.025 for 30% reduction of eGFR; adjusted HR 1.930, 95% CI 1.108 to 3.363, p=0.020 for 50% elevation of serum Cr).Conclusions: These data suggest that anti-CFH antibodies may be involved in the deterioration of renal function in primary MN.

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