Influence of lead acetate on seed germination and growth of young alfalfa plants

This study presents the results of testing the effect of different concentrations of lead-acetate on seed germination and young Seedlings of alfalfa. Тhe experiment was organized so that in the first variant, only lead-acetate of different concentrations (10-5, 10-4, 10-3, 10-2 and 2 x 10-2 M) was used, and in the second, next to the lead acetate EDTA concentration of 0.012 % was added. The results of this study in which natural conditions are imitated showed that the percentage of alfalfa seed germination decreases with increasing lead-acetate concentrations. In a medium of high concentration of lead acetate 10-2 and 2 x 10-2, a small number of seeds (39% and 32 (32%) germinated. In the same treatments with EDTA, the toxic effects of lead acetate were reduced, and seed germination was better (59% and 43% seeds germinated). It has been noticed that lead has toxic effects on the growth of alfalfa roots and stems. In the variant in which EDTA was used, the negative influence of lead on and growth of alfalfa seedlings was significantly mitigated.

Influence of lead acetate on seed germination and growth of young alfalfa plants

This study presents the results of testing the effect of different concentrations of lead-acetate on seed germination and young Seedlings of alfalfa. Тhe experiment was organized so that in the first variant, only lead-acetate of different concentrations (10-5, 10-4, 10-3, 10-2 and 2 x 10-2 M) was used, and in the second, next to the lead acetate EDTA concentration of 0.012 % was added. The results of this study in which natural conditions are imitated showed that the percentage of alfalfa seed germination decreases with increasing lead-acetate concentrations. In a medium of high concentration of lead acetate 10-2 and 2 x 10-2, a small number of seeds (39% and 32 (32%) germinated. In the same treatments with EDTA, the toxic effects of lead acetate were reduced, and seed germination was better (59% and 43% seeds germinated). It has been noticed that lead has toxic effects on the growth of alfalfa roots and stems. In the variant in which EDTA was used, the negative influence of lead on and growth of alfalfa seedlings was significantly mitigated.

Characterization of innately decellularised micropattern pseudostem of Musa balbisiana - A non-surface functionalized 3D economic biomaterial scaffold

Banana (Musa balbisiana) pseudostem 3D scaffolds have been developed here for primary eukaryotic cell and cell line culture as an economical, sustainable, eco-friendly alternative for surface-functionalized polymeric and plant tissue-based structures. Musa pseudostem 3D micro pattern scaffold (MPM-3Ds) developed by freeze-drying followed by ethylene oxide sterilization yielded 5.6ng of DNA per mg of tissue, confirming its extended decellularised state. Thermogravimetric analysis, contact angle measurement, uniaxial testing, and FTIR determined thermal stability, wettability, tensile strength, and surface functional groups respectively. Micro and macronutrients, sugars, and amino acids that naturally enrich MPM-3Ds were estimated using EDAX, HPLC, and biochemical analysis. The most important finding was, non-surface functionalized MPM-3Ds supported attachment, growth, and differentiation of human mesenchyme stem cells, human primary hepatocytes like cells, primary mouse brain cortical neurons, mouse fibroblast cells, and human pancreatic cancer cells. MPM-3Ds showed in vivo biodegradation and biocompatibility in a preliminary analysis in Sprague Dawley rats. These findings illuminate nature's power to nurture cells in the micropattern cradles of MPM- 3Ds that can support innovative research in stem cell differentiation, drug and cosmetic testing, and biosensor development leading to advanced biomedical research.

Characterization of innately decellularised micropattern pseudostem of Musa balbisiana - A non-surface functionalized 3D economic biomaterial scaffold

Banana (Musa balbisiana) pseudostem 3D scaffolds have been developed here for primary eukaryotic cell and cell line culture as an economical, sustainable, eco-friendly alternative for surface-functionalized polymeric and plant tissue-based structures. Musa pseudostem 3D micro pattern scaffold (MPM-3Ds) developed by freeze-drying followed by ethylene oxide sterilization yielded 5.6ng of DNA per mg of tissue, confirming its extended decellularised state. Thermogravimetric analysis, contact angle measurement, uniaxial testing, and FTIR determined thermal stability, wettability, tensile strength, and surface functional groups respectively. Micro and macronutrients, sugars, and amino acids that naturally enrich MPM-3Ds were estimated using EDAX, HPLC, and biochemical analysis. The most important finding was, non-surface functionalized MPM-3Ds supported attachment, growth, and differentiation of human mesenchyme stem cells, human primary hepatocytes like cells, primary mouse brain cortical neurons, mouse fibroblast cells, and human pancreatic cancer cells. MPM-3Ds showed in vivo biodegradation and biocompatibility in a preliminary analysis in Sprague Dawley rats. These findings illuminate nature's power to nurture cells in the micropattern cradles of MPM- 3Ds that can support innovative research in stem cell differentiation, drug and cosmetic testing, and biosensor development leading to advanced biomedical research.

Lacunae in the natural origin of severe acute respiratory syndrome coronavirus 2

The exact origin of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) responsible for unleashing the pandemic Corona Virus Disease (Covid-19) is still not established unambiguously. The intermediate and reservoir hosts of SARS-CoV-2 need to be identified with clarity and how the disease exploded into a pandemic, inevitability needs urgent scientific answers to contain and prevent future pandemics and crises. This perspective provides awareness of the peculiar features of SARS-CoV-2 and inspects the gaps in the natural zoonotic origin of the pandemic.

Production of bioethanol from amla (Emblica officinalis Gaertn.)

On account of the increasing demand for valuable herbal products, an attempt was made to produce a functional fermented Ethanol from Amla. This study investigates the potential of ethanol production from Amla (Emblica officinalis Gaertn). In the present study, Amla juice was extracted, filtered, fermented and it shows a suitable medium for the growth of Saccharomyces cerevisiae on yeast peptone dextrose medium for the production of ethanol. Ethanol was separated by fractional distillation and then estimated at 4, 6, 8 and 10 days of the fermentation process by iodometric method for 30oC. The ethanol percentage estimated by the iodometric titration method was high on the 10th day, and it was found to be 1.63 gm% compared to all days. So, the outcome of this study reveals that amla fruit can be used as a crucial constituent for the yield of ethanol with a higher commercial value.

Production of bioethanol from amla (Emblica officinalis Gaertn.)

On account of the increasing demand for valuable herbal products, an attempt was made to produce a functional fermented Ethanol from Amla. This study investigates the potential of ethanol production from Amla (Emblica officinalis Gaertn). In the present study, Amla juice was extracted, filtered, fermented and it shows a suitable medium for the growth of Saccharomyces cerevisiae on yeast peptone dextrose medium for the production of ethanol. Ethanol was separated by fractional distillation and then estimated at 4, 6, 8 and 10 days of the fermentation process by iodometric method for 30oC. The ethanol percentage estimated by the iodometric titration method was high on the 10th day, and it was found to be 1.63 gm% compared to all days. So, the outcome of this study reveals that amla fruit can be used as a crucial constituent for the yield of ethanol with a higher commercial value.

Lacunae in the natural origin of severe acute respiratory syndrome coronavirus 2

The exact origin of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) responsible for unleashing the pandemic Corona Virus Disease (Covid-19) is still not established unambiguously. The intermediate and reservoir hosts of SARS-CoV-2 need to be identified with clarity and how the disease exploded into a pandemic, inevitability needs urgent scientific answers to contain and prevent future pandemics and crises. This perspective provides awareness of the peculiar features of SARS-CoV-2 and inspects the gaps in the natural zoonotic origin of the pandemic.

Doxorubicin hydrochloride liposome and albumin-bound paclitaxel in cancer: a nanotechnology perspective

Nanoparticles (1-100 nanometres in size), products of nanotechnology, offer a modern way to transport anti-cancer drugs by acting as transporters of drugs into tumor cells, hence quenching tumor cell proliferation. Such nanoparticles may be formulated to bind to the tumor cell membrane or inhibit specific reactions of tumor biosynthetic pathway by gene repression, or directly bind to the active sites of essential enzymes in the biosynthetic pathway. Consequently, drugs are completely delivered to the desired cancerous cells without system interference. Liposomal doxorubicin and albumin-bound paclitaxel are two examples of nanotechnologically developed drugs for treating cancer. Modern knowledge of nanotechnology opens up new opportunities for innovative research on cancer therapies and administration and helps minimize harm to healthy cells. This review focuses on the doses and routes of administration of these chemotherapeutic agents used in treating cancers.

Impact of environmental conditions on the levels of stress and breeding performance in Wistar rats: conventional environment versus environmentally controlled housing.

It has been established that providing comparable and standard environmental conditions to house experimental rats is of high importance. However, there is growing evidence that rats prefer higher temperatures owing to their thermoneutral zone that lies between 28-34°C. This experiment validates the stress levels of Wistar rats of both sexes housed in conventional conditions about 24-31°C and a relative humidity of 70-92% in a tropical animal facility when compared to rats housed in controlled temperatures of 20-24°C with humidity maintained between 30-70%. Adequate air-changes to maintain ammonia-free environment was provided with a power-exhaust system in a conventional setup and with the individually ventilated caging system in the environmentally controlled group. To assess stress, faecal corticosteroid metabolite assay was done in naïve animals and in a set of animals that underwent retro-orbital bleeding under general anaesthesia. Mothering ability, fecundity and preweaning mortality were also compared between animals housed in two different conditions. Results showed no differences in stress hormone levels between the groups. However, faecal weights differed between males and females in both naïve and orbital bled groups. Animals housed in controlled environment clearly had better breeding data with lesser preweaning mortality in comparison to the conventionally housed rats. Further studies are required to elucidate whether the results are comparable when conducted during all seasons of the year at different locations across the globe.