Counting Cells in Sectioned Material: A Suite of Techniques, Tools, and Tips

2003 ◽  
Vol 24 (1) ◽  
Author(s):  
Robert W. Williams ◽  
Christopher S. Bartheld ◽  
Glenn D. Rosen
Keyword(s):  
Sectioned Material

A Freeze-Etch Study of Acinetobacter SP. Grown on a Hydrocarbon Substrate

1974 ◽  
Vol 32 ◽  
pp. 174-175
Author(s):  
C. L. Scott ◽  
W. R. Finnerty
Keyword(s):  
Membrane System ◽  
Structural Distortion ◽  
Intracytoplasmic Membrane ◽  
Gram Negative ◽  
Sectioned Material ◽  
Acinetobacter Sp

Acinetobacter sp. HO-1-N, a gram-negative hydrocarbon oxidizing bacterium previously designated Micrococcus cerificans, has been shown to sequester the hydrocarbon into intracytoplasmic pools as a result of growth on this substrate. In hydrocarbon grown cells, an intracytoplasmic membrane system was also observed along with a doubling of cellular phospholipids (Z). However, using conventional dehydration and embedding procedures in preparing thin sectioned material, the hydrocarbon is extracted from the cells. This may lead to structural distortion, consequently, the freeze-etch technique was applied to preserve the integrity of the cell.

An Efficient Statistic for Determining the Diameter of Thin Sectioned Uniform Spheres from Measurements of Biological Samples

1974 ◽  
Vol 32 ◽  
pp. 114-115
Author(s):  
W. R. Schucany ◽  
G. H. Kelsoe ◽  
V. F. Allison
Keyword(s):  
Biological Samples ◽  
Traditional Method ◽  
Cell Types ◽  
Accurate Estimation ◽  
Morphological Identification ◽  
Sectioned Material ◽  
Maximal Diameter ◽  
Similar Cell

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.

Surface Structure of the Spores of Glugea Weissenbergi

1969 ◽  
Vol 27 ◽  
pp. 238-239
Author(s):  
Sanford H. Vernick ◽  
Anastasios Tousimis ◽  
Victor Sprague
Keyword(s):  
Electron Microscope ◽  
Surface Structure ◽  
Transmission Electron Microscope ◽  
Mature Spore ◽  
Spore Surface ◽  
Sectioned Material ◽  
Transmission Electron ◽  
Surface Detail

Recent electron microscope studies have greatly expanded our knowledge of the structure of the Microsporida, particularly of the developing and mature spore. Since these studies involved mainly sectioned material, they have revealed much internal detail of the spores but relatively little surface detail. This report concerns observations on the spore surface by means of the transmission electron microscope.

Cytomembrane Preservation in Tissue Dehydrated Only With Glutaraldehyde and Epon 812 Resin

1973 ◽  
Vol 31 ◽  
pp. 320-321
Author(s):  
Daniel C. Pease
Keyword(s):  
Diffraction Pattern ◽  
X Ray Diffraction ◽  
High Concentration ◽  
Unpublished Study ◽  
X Ray ◽  
Sectioned Material

A previous study demonstrated that tissue could be successfully infiltrated with 50% glutaraldehyde, and then subsequently polymerized with urea to create an embedment which retained cytomembrane lipids in sectioned material. As a result, the 180-190 Å periodicity characteristic of fresh, mammalian myelin was preserved in sections, as was a brilliant birefringence, and the capacity to bind OsO4 vapor in the hydrophobic bilayers. An associated (unpublished) study, carried out in co-operation with Drs. C.K. Akers and D.F. Parsons, demonstrated that the high concentration of glutaraldehyde (and urea) did not significantly alter the X-ray diffraction pattern of aldehyde-fixed, myelin. Thus, by itself, 50% glutaraldehyde has little effect upon cytomembrane systems and can be used with confidence for the first stages of dehydration.

The abnormalities of the inner ear in kreisler mice

Development ◽  
1964 ◽  
Vol 12 (3) ◽  
pp. 475-490
Author(s):  
M. S. Deol
Keyword(s):  
Inner Ear ◽  
Recessive Gene ◽  
Cresyl Violet ◽  
Otic Vesicle ◽  
Litter Mate ◽  
Abnormal Position ◽  
Sectioned Material

The recessive gene kreisler (symbol kr) was discovered by Hertwig (1942a), who later described its effects on behaviour (1942b) and the inner ear (1944, 1956). She found that it was possible to trace the anomalies of the ear back to 9-day embryos, when the otic vesicle can be seen to be situated in an abnormal position. The present study was aimed at discovering the cause of this abnormality, and at giving a fuller account of the later development of the ear. Material and Methods The particulars of the sectioned material are given in Table 1. Only litter-mate controls were used throughout. The embryos were fixed in Bouin's fluid, sectioned at 7½ or 10 μ, depending on their age, and stained with Ehrlich's haematoxylin and eosin. The older material was fixed in Witmaack's fluid or formalin, sectioned at 10 μ, and stained either the same way or with cresyl violet or Weil's iron haematoxylin.

Ontogeny of dichotomizing apices in mycorrhizal short roots of Pinus strobus

1982 ◽  
Vol 60 (8) ◽  
pp. 1523-1528 ◽  
Author(s):  
Yves Piche ◽  
J. André Fortin ◽  
R. L. Peterson ◽  
U. Posluszny
Keyword(s):  
Pinus Strobus ◽  
Pisolithus Tinctorius ◽  
External Morphology ◽  
Lateral Growth ◽  
Central Group ◽  
Apical Meristems ◽  
Sectioned Material ◽  
Growth Centres

Growth pouches were used to establish ectomycorrhizae of Pinus strobus using Pisolithus tinctorius as inoculum. An epi-illumination technique was used to follow changes in external morphology of short roots as they became colonized by hyphae and dichotomized. Apices became broader and flatter before an obvious dichotomy was apparent. Secondary dichotomies often formed after the primary dichotomy of the original short roots. Sectioned material showed that a central group of cells in the meristem stopped dividing and became vacuolated as dichotomy was initiated. Two lateral growth centres were established which led to the formation of two apical meristems.

Observations on Wallrothiella arceuthobii, a fungus parasite of dwarf mistletoes

1969 ◽  
Vol 47 (9) ◽  
pp. 1359-1365 ◽  
Author(s):  
Job Kuijt
Keyword(s):  
Life Cycle ◽  
North America ◽  
Unknown Function ◽  
Western North America ◽  
Sectioned Material ◽  
Young Embryo ◽  
Spore Dissemination

Certain aspects of the life cycle of Wallrothiella arceuthobii are detailed from field and microscopical observations. Infected fruits of the host often continue to develop for several weeks after spore dissemination. Sectioned material shows that the fungus tends to avoid the young embryo and endosperm, yet attacks other adjacent mistletoe tissues. Perithecial shape varies considerably between eastern and western North America. Some western specimens show periostiolar locules of unknown function. The genus may be related to Coryneliaceae.

Cytochemistry and ultrastructure of the dehiscence zone of almond (Prunus dulcis) fruits

1990 ◽  
Vol 68 (1) ◽  
pp. 63-72 ◽  
Author(s):  
K. Weis ◽  
V. S. Polito
Keyword(s):  
Cell Wall ◽  
Fusion Zone ◽  
Prunus Dulcis ◽  
Epidermal Cells ◽  
Sectioned Material ◽  
Discrete Band ◽  
Dehiscence Zone ◽  
Suture Fusion ◽  
Cell Wall Hydrolases ◽  
Original Line

At maturity, the almond pericarp dehisces along the ventral suture, a region that originates by fusion of epidermal cells and subsequently differentiates into a separation layer. We have characterized the ontogeny of the fusion–dehiscence zone with emphasis on cell wall characteristics by using cytochemical methods for detection of pectin, cutin, cellulose, and lignin to examine the middle lamellae and primary and secondary walls in dehiscence-zone cells. Carpel margins became united postgenitally along opposing epidermal layers giving rise to the suture. Fusion-zone cells host epidermal characteristics, elaborated broad pectinaceous walls, and ultimately formed a discrete band of cells that dehisced along the original line of fusion by dissolution of cell wall pectins. Treatment of treeborne fruits with 1 ppm ethylene gas or extraction of sectioned material with cell wall hydrolases resulted in cell wall changes similar to those in predehiscent fruits.

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