Gellan Gum and Its Composites: Suitable Candidate for Efficient Nanodrug Delivery

SummaryThe APTT has been considered the most suitable candidate to monitor the anticoagulant activity of hirudin. However, its use is hampered by problems of standardization, which make the results heavily dependent on the responsiveness of the reagent used. Our aim was to investigate if this different responsiveness of different reagents when added in vitro is to be confirmed in an ex vivo study.Two different doses of r-hirudin (CGP 39393), 0.3 mg/kg and 1 mg/kg, were administered subcutaneously to 20 New Zealand male rabbits, and the differences in prolongation of APTT 2 and 12 h later were compared, using 8 widely used commercial reagents. All groups exhibited a significant prolongation of APTT 2 h after sc administration of hirudin, both at low and high doses. But this prolongation persisted 12 h later only when the PTTa reagent (Boehringer Mannheim) was used. In general, hirudin prolonged the APTT most with the silica- based reagents.In a further study, we compared the same APTT reagents in an in vitro study in which normal pooled plasma was mixed with increasing amount of hirudin. We failed to confirm a higher sensitivity for silica- containing reagents. Thus, we conclude that subcutaneous administration of hirudin prolongs the APTT most with the silica-based reagents, but this effect is exclusive for the ex vivo model.

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Evaluation of In Situ Gelation of Gellan Gum in Various Simulated Physiological Fluids

10.26226/morressier.57d6b2bbd462b8028d88d45d ◽

2016 ◽

Author(s):

Ramadan Diryak

Keyword(s):

Gellan Gum ◽

In Situ Gelation

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Preparation and Characterisation of Carvacrol Encapsulated in Gellan Gum Hydrogel

EDUCATUM Journal of Science, Mathematics and Technology ◽

10.37134/ejsmt.vol4.1.2.2017 ◽

2017 ◽

Vol 4 (1) ◽

pp. 9-14

Author(s):

Adila Mohamad Jaafar ◽

Norafida Hasnu ◽

Nur Aisyah Nasuha Mohd Azam

Keyword(s):

Gellan Gum

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a Novel Combinational Nanodrug Delivery System Induces Synergistic Inhibition of Lung Adenocarcinoma Cells in vitro

Letters in Drug Design & Discovery ◽

10.2174/1570180817999200719152426 ◽

2020 ◽

Vol 17 ◽

Author(s):

Mingliang Fan ◽

Jiping Li

Keyword(s):

Lung Adenocarcinoma ◽

Delivery System ◽

The Body ◽

Anticancer Efficacy ◽

Nanodrug Delivery ◽

Drug Molecules ◽

Cellular Assays ◽

Lung Adenocarcinoma Cell Line ◽

M Phase

Background: The combination of two or more therapeutic drugs is an attractive approach to improve the treatment of experimental tumors. Leveraging nanocarriers for combinational drug delivery can allow a control over drug biological fate and promote co-localization in the same area of the body. However, there are certain concerns regarding the biodegradability and potential long-term toxicity arising from these synthetic nanoscale carriers. Objective: Our aim was to develop a combinational nanodrug delivery system formed by self-assembling of amphiphilic drug molecules，minimizing potential toxicities associated with using additional synthetic nanocarriers. Methods: A novel prodrug chlorambucil gemcitabine conjugate was synthesized, this prodrug was used for the encapsulation of an additional hydrophobic anticancer drug paclitaxel, taking the form of combinational nanodrugs. Particle size and zeta potential were evaluated, cytotoxicity assay and apoptosis/cell cycle analysis were also performed to validate the anticancer efficacy of the combinational nanodrugs. Results: The combinational nanodrugs were acquired by means of nanoprecipitation. In A549 lung adenocarcinoma cell line, cellular assays revealed that co-delivery of low dosage paclitaxel with chlorambucil gemcitabine conjugate can act synergistically to inhibit cell growth and induce accumulation of cells in the G2/M phase with a concomitant decrease in G0/G1 compartment. Conclusion: Chlorambucil gemcitabine conjugate and paclitaxel can co-assemble into composite nanoparticles by a nanoprecipitation process and the resulting combinational nanodrugs showed synergistic anticancer effect. This synthetic nanocarrier-free approach might broaden the nanodrug concept and have potential in cancer therapy.

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Cancer Nanotechnology-An Excursion on Drug Delivery Systems

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520618666180720164015 ◽

2019 ◽

Vol 18 (15) ◽

pp. 2078-2092 ◽

Cited By ~ 2

Author(s):

Mala Sharma ◽

Chitranshu Pandey ◽

Neha Sharma ◽

Mohammad A. Kamal ◽

Usman Sayeed ◽

...

Keyword(s):

Drug Delivery ◽

Targeted Delivery ◽

Drug Delivery Systems ◽

Cancer Chemoprevention ◽

Delivery Systems ◽

Nanostructured Lipid Carrier ◽

Nanodrug Delivery ◽

Drug Conjugates ◽

Drug Nanoparticles ◽

Cancer Nanotechnology

Background: Nanotechnology pictures a breakthrough in the domain of cancer therapy owing to its novel properties and functions. This technology is quite amendable as it allows the scientists to engineer drug nanoparticles of dimensions 10nm – 500nm permitting them to pass via leaky vasculature of tumorigenic microenvironment with higher specificity, reduced cytotoxicity and effective release without any after effects. The central part of the review zooms onto the role of nanoparticles and their targeted delivery for the cure of cancer. Methods: The novel and various versatile nanoparticle platforms viz. polymeric (drug-conjugates, micelles, dendrimers), Lipid-based (liposomes, solid nanoparticle, nanostructured lipid carrier, lipid-polymer hybrid), and stimuli-sensitive (thermoresponsive, ultrasound, pH-responsive, hydrogel) etc. have been designed for a persistent, précised nanodrug delivery and the co-delivery of collegial drug conjugates leading to the formation of safer release of myriad of drugs for cancer chemoprevention. Results: The review concerns about tracing and detailing the drug delivery systems of cancer nanotechnology. Conclusion: Nanotechnology is bestowed with the design, depiction, fabrication, and application of nanostructures, and devices with their controlled delivery together with the imaging of the selected target site and drug release at the specific site of action.

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Co-polymerized Gellan Gum: A Carrier for Controlled Drug Delivery

Drug Delivery Letters ◽

10.2174/221030310503160401115805 ◽

2016 ◽

Vol 5 (3) ◽

pp. 151-162

Author(s):

Ranjeet Pareek ◽

Shakuntla Verma ◽

Viney Lather ◽

Deepti Pandita

Keyword(s):

Drug Delivery ◽

Controlled Drug Delivery ◽

Gellan Gum

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Chitosan Nanoparticles: An Approbative System for the Delivery of Herbal Bioactives

The Natural Products Journal ◽

10.2174/2210315510999201124155627 ◽

2020 ◽

Vol 10 ◽

Author(s):

Sapna Saini ◽

Sanju Nanda ◽

Anju Dhiman

Keyword(s):

Drug Delivery ◽

Drug Delivery Systems ◽

State Of The Art ◽

Chitosan Nanoparticles ◽

Delivery Systems ◽

Intrinsic Properties ◽

Amino Groups ◽

Suitable Candidate ◽

Film Forming ◽

Novel Drug

: Chitosan, a natural biodegradable polymer obtained from deacetylation of chitin, has been used as an approbative macromolecule for the development of various novel drug delivery systems. It is one of the most favorable biodegradable carriers for nanoparticulate drug delivery due to its intrinsic properties, such as biocompatibility, biodegradability, non-toxicity, availability of free reactive amino groups, and ease of chemical modification into different active derivatives. Furthermore, interesting physical properties (film-forming, gelling and thickening) make it a suitable candidate for formulations, such as films, microcapsules, beads, nanoparticles, nanofibres, nanogel and so on. Researchers have reported that chitosan nanoparticles act as a promising vehicle for herbal actives as they provide a superior alternative to traditional carriers and improve pharmaceutical efficiency. As no review of chitosan nanoparticles encapsulating herbal extracts and bioactives has been published till date, a maiden effort has been made to collate and review the use of chitosan nanoparticles for the entrapment of phytoconstituents to yield stable, efficient and safe drug delivery systems. Additionally, the paper presents a comprehensive account of the state-of the-art in fabricating herbal chitosan nanoparticles and their current pharmacological status. A list of patents on chitosan nanoparticles of herbal actives has also been included. This review is intended to serve as a didactic discourse for the formulation scientists endeavoring to develop advanced delivery systems for herbal actives.

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Performance Evaluation of Corporators and Authorities based on Public Grievances Redressal

International Journal of Knowledge Based Computer Systems ◽

10.21863/ijkbcs/2015.3.1.004 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Vanya Shree. B ◽

S. P. Shiva Prakash

Keyword(s):

Performance Evaluation ◽

Political Parties ◽

Water Supply ◽

Government Employees ◽

Suitable Candidate ◽

Drainage Connection ◽

Android App ◽

Timely Delivery

People in their day-to-day life face many general problems like damaged street lights, irregular water supply, blocked drainage connection etc. In these situations they complain to the concerned authority. If the complaint is unsolved, they complain to their areas representatives i.e. corporator. But in majority of the cases complainants dont get timely delivery of the solutions to their complaint and moreover authorities and corporators are neither made accountable nor answerable for it. Hence public grievances redressal is a major issue. Another important issue is that there are no quantitative measures representing a politician. If there would be any such metrics it would assist people in selecting suitable candidate in election and would also guide political parties in selecting suitable contestant for contesting in election. There are also no quantitative measures of government employees, which if exist would be used in providing promotions and increments. This work addresses these two major issues and provides solution for it. Android app is developed which evaluates Authorities and Corporators based on whether they have solved peoples complaint and if solved how early it is solved. It provides evaluation in terms of score and rank. It also provides performance graph and statistical graphs.

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