Simultaneous determination of molecular weight and crystallinity of recycled HDPE by infrared spectroscopy and multivariate calibration

2002 ◽  
Vol 85 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Walker Camacho ◽  
Sigbritt Karlsson
Keyword(s):  
Molecular Weight ◽  
Infrared Spectroscopy ◽  
Simultaneous Determination ◽  
Multivariate Calibration ◽  
Recycled Hdpe

Near infrared spectroscopy and multivariate calibration for simultaneous determination of glucose, triglycerides and high-density lipoprotein in animal plasma

2012 ◽  
Vol 66 ◽  
pp. 252-257 ◽  
Author(s):  
Ana Carolina de Oliveira Neves ◽  
Aurigena Antunes de Araújo ◽  
Bruna Laís Silva ◽  
Patrícia Valderrama ◽  
Paulo Henrique Março ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
High Density Lipoprotein ◽  
Simultaneous Determination ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Multivariate Calibration ◽  
Density Lipoprotein ◽  
High Density

scholarly journals Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3321
Author(s):  
Katarzyna Kurpet ◽  
Rafał Głowacki ◽  
Grażyna Chwatko
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Reversed Phase ◽  
Fluorescence Labeling ◽  
Detector Response ◽  
Low Molecular Weight

Biothiols are extremely powerful antioxidants that protect cells against the effects of oxidative stress. They are also considered relevant disease biomarkers, specifically risk factors for cardiovascular disease. In this paper, a new procedure for the simultaneous determination of human serum albumin and low-molecular-weight thiols in plasma is described. The method is based on the pre-column derivatization of analytes with a thiol-specific fluorescence labeling reagent, monobromobimane, followed by separation and quantification through reversed-phase high-performance liquid chromatography with fluorescence detection (excitation, 378 nm; emission, 492 nm). Prior to the derivatization step, the oxidized thiols are converted to their reduced forms by reductive cleavage with sodium borohydride. Linearity in the detector response for total thiols was observed in the following ranges: 1.76–30.0 mg mL−1 for human serum albumin, 0.29–5.0 nmol mL−1 for α-lipoic acid, 1.16–35 nmol mL−1 for glutathione, 9.83–450.0 nmol mL−1 for cysteine, 0.55–40.0 nmol mL−1 for homocysteine, 0.34–50.0 nmol mL−1 for N-acetyl-L-cysteine, and 1.45–45.0 nmol mL−1 for cysteinylglycine. Recovery values of 85.16–119.48% were recorded for all the analytes. The developed method is sensitive, repeatable, and linear within the expected ranges of total thiols. The devised procedure can be applied to plasma samples to monitor biochemical processes in various pathophysiological states.

Sign in / Sign up

Export Citation Format

Share Document