A novel method for obtaining a high-concentration chitosan solution and preparing a high-strength chitosan hollow-fiber membrane with an excellent adsorption capacity

2010 ◽  
Vol 115 (4) ◽  
pp. 1913-1921 ◽  
Author(s):  
Wei Han ◽  
Renbi Bai
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miku Ayano ◽  
Yoshiyuki Sawamura ◽  
Tomoko Hongo-Hirasaki ◽  
Takayuki Nishizaka

AbstractVirus removal filters developed for the decontamination of small viruses from biotherapeutic products are widely used in basic research and critical step for drug production due to their long-established quality and robust performance. A variety of imaging techniques have been employed to elucidate the mechanism(s) by which viruses are effectively captured by filter membranes, but they are limited to ‘static’ imaging. Here, we propose a novel method for detailed monitoring of ‘dynamic process’ of virus capture; specifically, direct examination of biomolecules during filtration under an ultra-stable optical microscope. Samples were fluorescently labeled and infused into a single hollow fiber membrane comprising cuprammonium regenerated-cellulose (Planova 20N). While proteins were able to pass through the membrane, virus-like particles (VLP) accumulated stably in a defined region of the membrane. After injecting the small amount of sample into the fiber membrane, the real-time process of trapping VLP in the membrane was quantified beyond the diffraction limit. The method presented here serves as a preliminary basis for determining optimum filtration conditions, and provides new insights into the structure of novel fiber membranes.


2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Shinji Hayashi ◽  
Rieko Yagi ◽  
Shuhei Taniguchi ◽  
Masami Uji ◽  
Hidaka Urano ◽  
...  

AbstractCell-assisted lipotransfer (CAL) is an advanced lipoinjection method that uses autologous lipotransfer with addition of a stromal vascular fraction (SVF) containing adipose-derived stromal stem cells (ASCs). The CAL procedure of manual isolation of cells from fat requires cell processing to be performed in clean environment. To isolate cells from fat without the need for a cell processing center, such as in a procedure in an operation theater, we developed a novel method for processing SVF using a closed cell washing concentration device (CCD) with a hollow fiber membrane module. The CCD consists of a sterilized closed circuit, bags and hollow fiber, semi-automatic device and the device allows removal of >99.97% of collagenase from SVF while maintaining sterility. The number of nucleated cells, ASCs and viability in SVF processed by this method were equivalent to those in SVF processed using conventional manual isolation. Our results suggest that the CCD system is as reliable as manual isolation and may also be useful for CAL. This approach will help in the development of regenerative medicine at clinics without a cell processing center.


2012 ◽  
Vol 512-515 ◽  
pp. 2308-2316 ◽  
Author(s):  
Zhen Wang ◽  
Meng Xiang Fang ◽  
Shui Ping Yan ◽  
Yi Li Pang ◽  
Zhong Yang Luo

Absorption of carbon dioxide (CO2) by blended diethanolamine (DEA) + 2-amino-2- methyl-1-propanol (AMP) and single DEA solvents were compared using hollow fiber membrane contactor (HFMC). Experimental results showed AMP additive has positive influence to improve CO2 absorption flux and the optimum AMP/DEA mass concentration ratio is between 0.2 and 0.4. Decreasing gas liquid ratio could greatly promote CO2 absorption, and operating temperature has weak effect on CO2 flux. Besides, large CO2 flux can be achieved with high concentration of DEA+0.2AMP solution due to the decrease of liquid phase resistance to mass transfer, but the optimal DEA concentration was recommended to be about 15% for DEA+0.2AMP solution considering the costs of amines in HFMC.


ASAIO Journal ◽  
1996 ◽  
Vol 42 (5) ◽  
pp. M446-450 ◽  
Author(s):  
LAURA W. LUND ◽  
WILLIAM J. FEDERSPIEL ◽  
FRANK R. WALTERS ◽  
BRACK G. HATTLER

2021 ◽  
Vol 47 (11) ◽  
pp. 15367-15382
Author(s):  
Mohamed Farag Twibi ◽  
Mohd Hafiz Dzarfan Othman ◽  
Siti Khadijah Hubadillah ◽  
Saber Abdulhamid Alftessi ◽  
Tonni Agustiono Kurniawan ◽  
...  

2017 ◽  
Vol 36 (1-2) ◽  
pp. 287-299 ◽  
Author(s):  
Changkun Liu ◽  
Jizhen Jia ◽  
Ji’an Liu ◽  
Xiaoyan Liang

A novel polypropylene hollow fiber membrane with a new function of selective adsorption of mercury ions in aqueous solutions was successfully prepared. The surface of the polypropylene hollow fiber membrane was initially modified with polydopamine by surface polymerization, and subsequently grafted with polyacrylamide (PAM) polymer brush via the surface initiated atom transfer radical polymerization (SI-ATRP) technique (thereafter named as PP-PAM). This study investigated the adsorption performance of Hg(II) ions by PP-PAM and the effect of various influencing factors on Hg(II) ion adsorption. The experiment indicated that the Hg(II) adsorption capacity of the PP-PAM increased with the increase of the pH, and the Hg(II) adsorption kinetics was consistent with the pseudo-second-order kinetic model. The adsorption isotherm followed the Langmuir model, with the maximum adsorption capacity calculated to be 0.854 mmol/g for Hg(II) ions. The adsorption study in multi-component system indicated that PP-PAM preferentially adsorbs Hg(II) over Pb(II) ions, with significant adsorption capacity difference of the two heavy metal ions. This study provided an efficient method for the preparation of the adsorptive polypropylene hollow fiber membrane, which expands its application for the selective removal of heavy metal ions.


RSC Advances ◽  
2017 ◽  
Vol 7 (17) ◽  
pp. 10424-10431 ◽  
Author(s):  
S. S. Shen ◽  
J. J. Yang ◽  
C. X. Liu ◽  
R. B. Bai

The prepared IMAM (CS/CA-Cu) achieved a high adsorption capacity of 69 mg-BSA per g-membrane.


Sign in / Sign up

Export Citation Format

Share Document