Novel spectrofluorimetric technique for determination of amoxicillin and ethopabate in chicken tissues, liver, kidney, eggs and feed premix

Abstract A sensitive and specific gas-liquid chromatographic (GLC) method has been developed for determining low levels of clopidol in chicken tissues. Clopidol is extracted from the tissues with methanol, and cleaned up on an alumina column and an anion exchange resin column with 0.1% acetic acid–methanol as eluate. Clopidol is methylated with diazomethane, and then determined by GLC. 2,4-Dinitro-l-chlorobenzene is used as an internal standard. The method is applicable to levels as low as 2 ppb in chicken tissues. Recoveries of 2–20 ppb clopidol added to tissues averaged 87% for muscle, 84% for liver, 80% for kidney, and 76% for fat.

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LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/bvip-2014-0089 ◽

2014 ◽

Vol 58 (4) ◽

pp. 573-579 ◽

Cited By ~ 9

Author(s):

Anna Gajda ◽

Andrzej Posyniak ◽

Grzegorz Tomczyk

Keyword(s):

Oral Administration ◽

Broiler Chickens ◽

Thigh Muscle ◽

Limit Of Quantification ◽

Limit Values ◽

Chicken Tissues ◽

Short Period ◽

Liquid Chromatography Tandem Mass ◽

Liver And Kidney

Abstract For the purpose of quantitative determination of doxycycline (DC) residues in tissues, a sensitive liquid chromatography - tandem mass spectrometry (LC-MS/MS) method was developed. The method was used to determine DC residues in chicken tissues (breast and thigh muscle, liver and kidney) after oral administration with drinking water to five-weak-old broiler chickens. The DC was administered for five consecutive days at a therapeutic dose of 10 mg/kg b.w. once a day. The tissues were collected after 6 h, 24 h, 7 d, and 8 d. The method was validated and the decision limit was established for muscle - 109.2 μg/kg, for liver - 326.1 μg/kg, and for kidney - 634.0 μg/kg. The detection limit was 2 μg/kg and the limit of quantification was 5 μg/kg. In a short period after ceasing the treatment, the detected concentrations of DC were much higher than the established maximum residue limit values. The highest residue concentrations of DC were observed in the kidney, followed by the liver and muscle. The lowest concentration of DC was determined in tight muscle.

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Determination of Norfloxacin in Chicken Tissues by HPLC with Fluorescence Detection

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1081/jlc-200038615 ◽

2005 ◽

Vol 28 (1) ◽

pp. 121-135 ◽

Cited By ~ 15

Author(s):

C. Kowalski ◽

Z. Roliński ◽

T. Sławik ◽

B. K. Głód

Keyword(s):

Fluorescence Detection ◽

Chicken Tissues

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Determination of Monensin in Chicken Tissues by Liquid Chromatography with Postcolumn Derivatization

Journal of AOAC International ◽

10.1093/jaoac/77.4.885 ◽

1994 ◽

Vol 77 (4) ◽

pp. 885-890 ◽

Cited By ~ 13

Author(s):

John W Moran ◽

J Matthew Rodewald ◽

Alvin L Donoho ◽

Mark R Coleman

Keyword(s):

Liquid Chromatography ◽

Silica Gel ◽

Chicken Tissues ◽

Chicken Muscle ◽

Limit Of Quantitation ◽

Microbiological Techniques ◽

Wavelength Detector ◽

Acidic Conditions ◽

Postcolumn Derivatization

Abstract A method is described for the detection and quantitation of monensin in chicken tissues by liquid chromatography with postcolumn derivatization with vanillin. Monensin is extracted from the tissues by homogenization with methanol–water and is isolated and concentrated by liquid–liquid partition and sorbent extraction with silica gel. Monensin is mixed postcolumn with vanillin under acidic conditions and heated, and the resulting products are measured by a variable-wavelength detector operating at 520 nm. The method has a limit of quantitation of 0.025 μg/g and is validated for use in the analyses of chicken muscle, liver, and skin (with adhering fat tissues) for monensin. Standard recoveries from the 3 tissue types tested at 3 levels ranged from 82 to 96%. The method represents an improvement in specificity, accuracy, and analysis time over existing methods, which use microbiological techniques.

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Liquid Chromatographic Determination of Spiramycin Residues in Chicken Tissues

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/69.4.644 ◽

1986 ◽

Vol 69 (4) ◽

pp. 644-646

Author(s):

Tomoko Nagata ◽

Masanobu Saeki

Keyword(s):

Detection Limit ◽

Ethyl Ether ◽

Chromatographic Determination ◽

Chicken Tissues ◽

Chicken Muscles ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for determination of spiramycin residues in chicken muscles. The drug is extracted from muscles with acetonitrile, the extract is concentrated to 3-4 mL and rinsed with n-hexane followed by ethyl ether, and the drug is extracted with chloroform. LC analysis is carried out on a Zorbax BP-C8 column, and spiramycin is detected spectrophotometrically at 231 nm. Recoveries of spiramycin added to chicken muscles at 0.2 and 0.1 ppm were 93.9 and 89.0%, respectively. The detection limit was 5 ng for spiramycin standard, and 0.05 ppm in chicken muscles.

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Liquid Chromatographic Determination of Monensin in Chicken Tissues with Fluorometric Detection and Confirmation by Gas Chromatography-Mass Spectrometry

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/69.3.443 ◽

1986 ◽

Vol 69 (3) ◽

pp. 443-448

Author(s):

Keigo Takatsuki ◽

Shigeru Suzuki ◽

Isamu Ushizawa

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Trimethylsilyl Ether ◽

Phosphate Buffer Solution ◽

Fluorescent Labeling ◽

Gas Chromatography Mass Spectrometry ◽

Fluorometric Detection ◽

Chicken Tissues ◽

Chromatography Mass Spectrometry

Abstract An accurate, sensitive method is described for the determination of monensin residue in chicken tissues by liquid chromatography (LC), in which monensin is derivatized with a fluorescent labeling reagent, 9-anthryldiazomethane (ADAM), to enable fluorometric detection. Samples are extracted with methanol-water (8 + 2), the extract is partitioned between CHCl3 and water, and the CHC13 layer is cleaned up by silica gel column chromatography. Free monensin, obtained by treatment with phosphate buffer solution (pH 3) at 0°C, is derivatized with ADAM and passed through a disposable silica cartridge. Monensin-ADAM is identified and quantitated by normal phase LC using fluorometric detection. The detection limit is 1 ppb in chicken tissues. Recoveries were 77.6 ± 1.8% at 1 ppm, 56.7 ± 7.1% at 100 ppb, and 46.5 ± 3.7% at 10 ppb fortification levels in chicken. Gas chromatography-mass spectrometry is capable of confirming monensin methyl ester tris trimethylsilyl ether in samples containing residues >5 ppm.

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