scholarly journals Meta-Analysis Combines Affymetrix Microarray Results Across Laboratories

2005 ◽  
Vol 6 (3) ◽  
pp. 116-122 ◽  
Author(s):  
John R. Stevens ◽  
R. W. Doerge
Keyword(s):  
Meta Analysis ◽  
Gene List ◽  
Analytic Approach ◽  
Oligonucleotide Array ◽  
Microarray Technology ◽  
Affymetrix Platform ◽  
Affymetrix Microarray ◽  
Significant Interest ◽  
Affymetrix Oligonucleotide Array ◽  
Adaptive Nature

With microarray technology becoming more prevalent in recent years, it is now common for several laboratories to employ the same microarray technology to identify differentially expressed genes that are related to the same phenomenon in the same species. Although experimental specifics may be similar, each laboratory will typically produce a slightly different list of statistically significant genes, which calls into question the validity of each gene list (i.e. which list is best). A statistically-based meta-analytic approach to microarray analysis systematically combines results from the different laboratories to provide a single estimate of the degree of differential expression for each gene. This approach provides a more precise view of genes that are of significant interest, while simultaneously allowing for differences between laboratories. The widely-used Affymetrix oligonucleotide array and its software are of particular interest because the results are naturally suited to a meta-analysis. A simulation model based on the Affymetrix platform is developed to examine the adaptive nature of the meta-analytic approach and to illustrate the utility of such an approach in combining microarray results across laboratories.

MMP20 rs1784418 Protects Certain Populations against Caries

2016 ◽  
Vol 51 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Arnôldo V.A. Filho ◽  
Merilene S. Calixto ◽  
Kathleen Deeley ◽  
Neide Santos ◽  
Aronita Rosenblatt ◽  
...  
Keyword(s):  
Risk Factor ◽  
Dental Caries ◽  
Dna Extraction ◽  
Meta Analysis ◽  
Primary Dentition ◽  
Analytic Approach ◽  
Caries Experience ◽  
Statistical Approaches

This work aimed to further evaluate the association of MMP20 rs1784418 C>T and dental caries experience with the hypothesis that MMP20 rs1784418 C>T is a risk factor for dental caries. 184 children 4-7 years of age had their caries experience determined and buccal cheek swabs collected for DNA extraction to test for association with the MMP20 rs1784418 C>T using standard statistical approaches. A meta-analytic approach was also implemented to compile previous discrepant reports of the same association. We found an association between MMP20 rs1784418 C>T and dental caries experience in primary dentition (p = 0.01). The meta-analysis showed that this association appears to favor individuals born in Brazil and not Turkey. MMP20 rs1784418 C>T appears to protect against dental caries, but its effects are likely to be more marked in certain populations.

scholarly journals Disentangling ADHD's Presentation-Related Decision-Making—A Meta-Analytic Approach on Predominant Presentations

2021 ◽  
Vol 12 ◽  
Author(s):  
Marcel Schulze ◽  
David Coghill ◽  
Silke Lux ◽  
Alexandra Philipsen
Keyword(s):  
Decision Making ◽  
Attention Deficit Hyperactivity Disorder ◽  
Reinforcement Learning ◽  
Risk Taking ◽  
Attention Deficit ◽  
Meta Analysis ◽  
Analytic Approach ◽  
Effect Sizes ◽  
Hyperactivity Disorder ◽  
The Relationship

Background: Deficient decision-making (DM) in attention deficit/hyperactivity disorder (ADHD) is marked by altered reward sensitivity, higher risk taking, and aberrant reinforcement learning. Previous meta-analysis aggregate findings for the ADHD combined presentation (ADHD-C) mostly, while the ADHD predominantly inattentive presentation (ADHD-I) and the predominantly hyperactive/impulsive presentation (ADHD-H) were not disentangled. The objectives of the current meta-analysis were to aggregate findings from DM for each presentation separately.Methods: A comprehensive literature search of the PubMed (Medline) and Web of Science Database took place using the keywords “ADHD,” “attention-deficit/hyperactivity disorder,” “decision-making,” “risk-taking,” “reinforcement learning,” and “risky.” Random-effects models based on correlational effect-sizes were conducted. Heterogeneity analysis and sensitivity/outlier analysis were performed, and publication biases were assessed with funnel-plots and the egger intercept.Results: Of 1,240 candidate articles, seven fulfilled criteria for analysis of ADHD-C (N = 193), seven for ADHD-I (N = 256), and eight for ADHD-H (N = 231). Moderate effect-size were found for ADHD-C (r = 0.34; p = 0.0001; 95% CI = [0.19, 0.49]). Small effect-sizes were found for ADHD-I (r = 0.09; p = 0.0001; 95% CI = [0.008, 0.25]) and for ADHD-H (r = 0.1; p = 0.0001; 95% CI = [−0.012, 0.32]). Heterogeneity was moderate for ADHD-H. Sensitivity analyses show robustness of the analysis, and no outliers were detected. No publication bias was evident.Conclusion: This is the first study that uses a meta-analytic approach to investigate the relationship between the different presentations of ADHD separately. These findings provide first evidence of lesser pronounced impairment in DM for ADHD-I and ADHD-I compared to ADHD-C. While the exact factors remain elusive, the current study can be considered as a starting point to reveal the relationship of ADHD presentations and DM more detailed.

scholarly journals Trends in Inflammatory Bowel Disease Therapy: A Meta-Analytic Approach

1995 ◽  
Vol 9 (7) ◽  
pp. 405-411 ◽  
Author(s):  
Gregory W Whiting ◽  
Joseph Lau ◽  
Bruce Kupelnick ◽  
Thomas C Chalmers
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Meta Analysis ◽  
Statistical Significance ◽  
Analytic Approach ◽  
Disease Therapy ◽  
The Matrix ◽  
Inflammatory Bowel ◽  
Acute Attacks ◽  
New Treatments

OBJECTIVE: To illustrate the current meta-analytic approach to extracting clinically useful information from the glut of randomized controlled trials (RCTs) of the treatment of acute attacks of Crohn's disease.PROCEDURE: Acceptable RCTs were classified in a matrical format so that the feasibility of a meta-analysis of each treatment could be quickly determined. Each potential inclusion was then photocopied after blinding the source and outcome to minimize bias by the investigators.RESULTS AND CONCLUSIONS: The search was started in 1990, when a previous meta-analysis was completed. Over 100 citations in MEDLINE contained 21 RCTs of the treatment of acute attacks of Crohn's disease, and 11 more were culled from the references of recent papers. The meta-analysis most indicated by the matrix was a comparison of several different immunosuppressive regimens with a placebo. The previously demonstrated statistical significance was confirmed and the point made that it is no longer justified to compare any new treatments with a placebo. Cumulative meta-analysis, the process of performing a new analysis every time a new trial is published, revealed the importance of employing both random and fixed effect models when heterogeneity of treatment results is appreciable, as in this case.

scholarly journals Taste receptor polymorphisms and longevity: a systematic review and meta-analysis

2020 ◽  
Author(s):  
Danilo Di Bona ◽  
Alberto Malovini ◽  
Giulia Accardi ◽  
Anna Aiello ◽  
Giuseppina Candore ◽  
...  
Keyword(s):  
Systematic Review ◽  
Bitter Taste ◽  
Meta Analysis ◽  
Taste Receptor ◽  
The Other ◽  
Analytic Approach ◽  
Taste Receptors ◽  
South Italy ◽  
Physiological Processes ◽  
Different Populations

AbstractBitter taste receptors (TAS2R) are involved in a variety of non-tasting physiological processes, including immune-inflammatory ones. Therefore, their genetic variations might influence various traits. In particular, in different populations of South Italy (Calabria, Cilento, and Sardinia), polymorphisms of TAS2R16 and TAS238 have been analysed in association with longevity with inconsistent results. A meta-analytic approach to quantitatively synthesize the possible effect of the previous variants and, possibly, to reconcile the inconsistencies has been used in the present paper. TAS2R38 variants in the Cilento population were also analysed for their possible association with longevity and the obtained data have been included in the relative meta-analysis. In population from Cilento no association was found between TAS2R38 and longevity, and no association was observed as well, performing the meta-analysis with data of the other studies. Concerning TAS2R16 gene, instead, the genotype associated with longevity in the Calabria population maintained its significance in the meta-analysis with data from Cilento population, that, alone, were not significant in the previously published study. In conclusion, our results suggest that TAS2R16 genotype variant is associated with longevity in South Italy.

A Meta-Analysis of the Effects of Glycerol-Induced Hyperhydration on Fluid Retention and Endurance Performance

2007 ◽  
Vol 17 (4) ◽  
pp. 391-410 ◽  
Author(s):  
Eric D.B. Goulet ◽  
Mylène Aubertin-Leheudre ◽  
Gérard E. Plante ◽  
Isabelle J. Dionne
Keyword(s):  
Body Weight ◽  
Effect Size ◽  
Meta Analysis ◽  
Endurance Performance ◽  
Fluid Retention ◽  
Analytic Approach ◽  
Better Than

The authors determined, through a meta-analytic approach, whether glycerol-induced hyperhydration (GIH) enhances fluid retention and increases endurance performance (EP) significantly more than water-induced hyperhydration (WIH). Collectively, studies administered 23.9 ± 2.7 mL of fuid/kg body weight (BW) with 1.1 ± 0.2 g glycerol/kg BW, and hyperhydration was measured 136 ± 15 min after its onset. Compared with WIH, GIH increased fluid retention by 7.7 ± 2.8 mL/kg BW (P < 0.01; pooled effect size [PES]: 1.64 ± 0.80, P < 0.01, N = 14). The use of GIH was associated with an improvement in EP of 2.62% ± 1.60% (P = 0.047; PES: 0.35 ± 0.13, P = 0.014, N = 4). Unarguably, GIH significantly enhances fluid retention better than WIH. Because of the dearth of data, the effect of GIH on EP must be further investigated before more definitive conclusions can be drawn as to its ergogenic property.

Surrogates, meta-analysis and cost-effectiveness modelling: a combined analytic approach

2011 ◽  
Vol 21 (6) ◽  
pp. 742-756 ◽  
Author(s):  
Neil Hawkins ◽  
Gerry Richardson ◽  
Alex J Sutton ◽  
Nicola J Cooper ◽  
Chris Griffiths ◽  
...  
Keyword(s):  
Cost Effectiveness ◽  
Meta Analysis ◽  
Analytic Approach

scholarly journals Robust rank aggregation for gene list integration and meta-analysis

2012 ◽  
Vol 28 (4) ◽  
pp. 573-580 ◽  
Author(s):  
Raivo Kolde ◽  
Sven Laur ◽  
Priit Adler ◽  
Jaak Vilo
Keyword(s):  
Meta Analysis ◽  
Gene List ◽  
Rank Aggregation

Narrative Versus Meta-Analytic Reviews of Race Differences in Motivation: A Comment on Cooper and Dorr

1995 ◽  
Vol 65 (4) ◽  
pp. 509-514 ◽  
Author(s):  
Sandra Graham
Keyword(s):  
Meta Analysis ◽  
Race Differences ◽  
Analytic Approach ◽  
Effect Sizes ◽  
Narrative Approach ◽  
Need For Achievement ◽  
Psychological Variable ◽  
High Quality ◽  
Study Characteristics ◽  
Score Method

Some of the benefits and shortcomings of a meta-analytic approach to reviewing race differences in need for achievement ( Cooper & Dorr, 1995 ) are examined and compared to the narrative approach that I adopted in a previous review on this topic ( Graham, 1994 ). Among the benefits of meta-analysis are the calculation of effect sizes for race differences (compared to the box score method of my narrative review) and the presentation of replicable and objective procedures for organizing, describing, and comparing study characteristics. Among the perceived limitations are the meta-analyst’s reluctance to distinguish between low- and high-quality studies and an overemphasis on quantitative comparisons of substantively disparate literatures. The implications for studying race as a psychological variable are also discussed.

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