Artificial Nucleobase-Amino Acid Conjugates: A New Class of TAR RNA Binding Agents

The impact of the amino-acid side-chain length on peptide–RNA binding events has been investigated using HIV-1 Tat derived peptides as ligands and the HIV-1 TAR RNA element as an RNA model.

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Fluoroquinolone-3-carboxamide Amino Acid Conjugates: Synthesis, Antibacterial Properties And Molecular Modeling Studies

Medicinal Chemistry ◽

10.2174/1573406415666190904143852 ◽

2020 ◽

Vol 17 (1) ◽

pp. 71-84

Author(s):

Riham M. Bokhtia ◽

Siva S. Panda ◽

Adel S. Girgis ◽

Hitesh H. Honkanadavar ◽

Tarek S. Ibrahim ◽

...

Keyword(s):

Experimental Data ◽

Antibacterial Activity ◽

Amino Acid ◽

Bacterial Infections ◽

Antibacterial Agents ◽

Antibacterial Properties ◽

Computational Studies ◽

Protecting Group ◽

Amino Acid Conjugates ◽

Molecular Modeling Studies

Background: Bacterial infections are considered as one of the major global health threats, so it is very essential to design and develop new antibacterial agents to overcome the drawbacks of existing antibacterial agents. Method: The aim of this work is to synthesize a series of new fluoroquinolone-3-carboxamide amino acid conjugates by molecular hybridization. We utilized benzotriazole chemistry to synthesize the desired hybrid conjugates. Result: All the conjugates were synthesized in good yields, characterized, evaluated for their antibacterial activity. The compounds were screened for their antibacterial activity using methods adapted from the Clinical and Laboratory Standards Institute. Synthesized conjugates were tested for activity against medically relevant pathogens; Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27856) Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 19433). Conclusion: The observed antibacterial experimental data indicates the selectivity of our synthesized conjugates against E.Coli. The protecting group on amino acids decreases the antibacterial activity. The synthesized conjugates are non-toxic to the normal cell lines. The experimental data were supported by computational studies.

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N-(Fluorenyl-9-methoxycarbonyl)amino Acid Amide Derivatives as a New Class of Anti-cancer Agents

Advances in Experimental Medicine and Biology - Peptides for Youth ◽

10.1007/978-0-387-73657-0_200 ◽

2009 ◽

pp. 465-466 ◽

Cited By ~ 3

Author(s):

Lajos Gera ◽

Daniel C. Chan ◽

Vitalija Simkeviciene ◽

Paul A. Jr Bunn ◽

John M. Stewart

Keyword(s):

Amino Acid ◽

Acid Amide ◽

Amino Acid Amide ◽

New Class ◽

Amide Derivatives ◽

Anti Cancer

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Nicotinamide riboside–amino acid conjugates that are stable to purine nucleoside phosphorylase

Organic & Biomolecular Chemistry ◽

10.1039/d0ob00134a ◽

2020 ◽

Vol 18 (15) ◽

pp. 2877-2885

Author(s):

Faisal Hayat ◽

Marie E. Migaud

Keyword(s):

Amino Acid ◽

Acid Ester ◽

Purine Nucleoside Phosphorylase ◽

Amino Acid Ester ◽

Purine Nucleoside ◽

Nucleoside Phosphorylase ◽

Amino Acid Conjugates ◽

Nicotinamide Riboside

O5′ amino acid ester conjugates of nicotinamide riboside, generated via a reduced intermediate, are stable to purine nucleoside phosphorylase.

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Sequence and structure of mtr, an amino acid transport gene of Neurospora crassa

Genome ◽

10.1139/g91-098 ◽

1991 ◽

Vol 34 (4) ◽

pp. 644-651 ◽

Cited By ~ 16

Author(s):

Kenneth Koo ◽

W. Dorsey Stuart

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Neurospora Crassa ◽

Rna Binding ◽

Signal Sequence ◽

Average Length ◽

Open Reading Frame ◽

Mrna Transcript ◽

S1 Nuclease ◽

Reading Frame

The gene product of the mtr locus of Neurospora crassa is required for the transport of neutral aliphatic and aromatic amino acids via the N system. We have previously cloned three cosmids containing Neurospora DNA that complement the mtr-6(r) mutant allele. The cloned DNAs were tightly linked to restriction fragment length polymorphisms that flank the mtr locus. A 2.9-kbp fragment from one cosmid was subcloned and found to complement the mtr-6(r) allele. Here we report the sequence of the fragment that hybridized to a poly(A)+ mRNA transcript of about 2300 nucleotides. We have identified an 845-bp open reading frame (ORF) having a 59-bp intron as the potential mtr ORF. S1 nuclease analysis of the transcript confirmed the transcript size and the presence of the intron. A second open reading frame was found upstream in the same reading frame as the mtr ORF and appears to be present in the mRNA transcript. The mtr ORF is predicted to encode a 261 amino acid polypeptide with a molecular mass of 28 613 Da. The proposed polypeptide exhibits six potential α-helical transmembrane domains with an average length of 23 amino acids, does not have a signal sequence, and contains amino acid sequence homologous to an RNA binding motif.Key words: sequence, membranes, ribonucleoprotein.

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Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR

Science ◽

10.1126/science.2011739 ◽

1991 ◽

Vol 251 (5001) ◽

pp. 1597-1600 ◽

Cited By ~ 242

Author(s):

A Gatignol ◽

A Buckler-White ◽

B Berkhout ◽

K. Jeang

Keyword(s):

Rna Binding ◽

Binding Protein ◽

Rna Binding Protein ◽

Tar Rna ◽

Hiv 1

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The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2016.10.023 ◽

2016 ◽

Vol 480 (2) ◽

pp. 187-193 ◽

Cited By ~ 13

Author(s):

Akihiko Komuro ◽

Yuya Homma ◽

Takaharu Negoro ◽

Glen N. Barber ◽

Curt M. Horvath

Keyword(s):

Rna Binding ◽

Binding Protein ◽

Rna Binding Protein ◽

Tar Rna

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Comprehensive landscape and future perspectives of circular RNAs in colorectal cancer

Molecular Cancer ◽

10.1186/s12943-021-01318-6 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Fei Long ◽

Zhi Lin ◽

Liang Li ◽

Min Ma ◽

Zhixing Lu ◽

...

Keyword(s):

Colorectal Cancer ◽

Molecular Mechanisms ◽

Rna Binding ◽

Rna Binding Proteins ◽

Circular Rnas ◽

Future Perspectives ◽

Cis Acting ◽

New Class ◽

Functional Peptides ◽

Mirna Sponges

AbstractColorectal cancer (CRC) is a common hereditary tumor that is often fatal. Its pathogenesis involves multiple genes, including circular RNAs (circRNAs). Notably, circRNAs constitute a new class of noncoding RNAs (ncRNAs) with a covalently closed loop structure and have been characterized as stable, conserved molecules that are abundantly expressed in tissue/development-specific patterns in eukaryotes. Based on accumulating evidence, circRNAs are aberrantly expressed in CRC tissues, cells, exosomes, and blood from patients with CRC. Moreover, numerous circRNAs have been identified as either oncogenes or tumor suppressors that mediate tumorigenesis, metastasis and chemoradiation resistance in CRC. Although the regulatory mechanisms of circRNA biogenesis and functions remain fairly elusive, interesting results have been obtained in studies investigating CRC. In particular, the expression of circRNAs in CRC is comprehensively modulated by multiple factors, such as splicing factors, transcription factors, specific enzymes and cis-acting elements. More importantly, circRNAs exert pivotal effects on CRC through various mechanisms, including acting as miRNA sponges or decoys, interacting with RNA binding proteins, and even translating functional peptides. Finally, circRNAs may serve as promising diagnostic and prognostic biomarkers and potential therapeutic targets in the clinical practice of CRC. In this review, we discuss the dysregulation, functions and clinical significance of circRNAs in CRC and further discuss the molecular mechanisms by which circRNAs exert their functions and how their expression is regulated. Based on this review, we hope to reveal the functions of circRNAs in the initiation and progression of cancer and highlight the future perspectives on strategies targeting circRNAs in cancer research.

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