scholarly journals Increased eDNA detection sensitivity using a novel high‐volume water sampling method

2020 ◽  
Vol 2 (2) ◽  
pp. 244-251
Author(s):  
Jenna C. Schabacker ◽  
Stephen J. Amish ◽  
Bonnie K. Ellis ◽  
Beth Gardner ◽  
Dulaney L. Miller ◽  
...  
2017 ◽  
Vol 4 (1) ◽  
pp. 151
Author(s):  
Hasanuddin Parulian Sihombing ◽  
I Gede Hendrawan ◽  
Yulianto Suteja

Lemuru fish is one of fishery commodity that has high economical value and one of fish that most catched by fisherman in Bali Strait. Lemuru fish had been caught in Bali Strait was fluctuating every month and every years. This condition was related with food source of Lemuru fish such as phytoplankton and zooplankton. So this research was conducted to explained the relationship phytoplankton and zooplankton abundance with Lemuru fish catched  in Bali strait. This study focus in Bali strait during March until May 2017. Determination of sampling point used area sampling method while water sampling occured in surface water with pouring method. Total of phytoplankton and zooplankton abundance in Bali strait in March until May had formed the sinusoidal model with their abundance ranged 301 ind/L – 604 ind/L and 7 ind/L – 12 ind/L. Plankton abundance in Bali strait in March until May (transisonal season 1) was categorized low abundance if compared with plankton abundance in another season. The low value of phytoplankton abundance caused by non upwelling phenomenon and grazing process and the low abundance of zooplankton caused by low rate of zooplankton and predation by Lemuru fish. Phytoplankton and zooplankton abundance had  strong relationship with Lemuru fish catched with correlation coefficient value 0.76 and 0.69. This condition caused by phytoplankton and zooplankton are source of Lemuru fish food.


2019 ◽  
Vol 8 (1) ◽  
pp. 57-66
Author(s):  
Reza Iklima AS ◽  
Gusti Diansyah ◽  
Andi Agussalim ◽  
Citra Mulia

Iklima AS et al, 2019. Analysis of N-Nitrogen (Ammonia, Nitrate, and Nitric) and Phosphate at Teluk Pandan’s water territorial, Lampung Province. JLSO 8(1):57-66.Teluk Pandan’s water territorial was known to aquaculture activity such as prawn, pearl oyster and cage culture by community that lived in the area. It activities could makes water quality to be polluted.This research was purposed to known the content of nutrient (Ammonia, Nitrate, Nitric, and Phosphate) and to studied nutrient that related to other’s water quality parametric at Teluk Pandan water territorial. Sampling was determinate by 15station using purposive sampling method. Data analysis was used to studied relation between water quality’s parametric using Principal Component Analysis (PCA). Water sampling was taken at surface using water sampler. It was analyze in Oceanography and Instrumentation Laboratory, Department of Marine Science, Universitas Sriwijaya. Result of this research showing that rate of content nutrient at Teluk Pandan’s water territory ranging from 0.0007-0.0087 mg/L NO3-N, nitric ranging from 0.0001-0.0062 mg/L NO2-N, and phosphate ranging form 0,0012 – 0,0091 mg/L PO4-P. Based on result Teluk Pandan’s water territory still can be used for water’s ecosystem. Result using PCA method showing that correlation between parametric are directly proportional and inversely. Correlation that directly proportional showing by parametric group quadrant I (Temperature, Salinity, Velocity, and Abundance of Phytoplankton), quadrant II (DO, pH and nitrate) and quadrant III (Ammonia, nitric and phosphate). Inversely showing by parametric group quadrant I to parametric group quadrant III.


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 676a-676
Author(s):  
Karen E. Williams ◽  
John L. Cisar ◽  
George H. Snyder

Current interest in the fate of agrochemicals applied to turf is encouraging many turf scientists to contemplate renovation of existing field plots for soil-water monitoring studies. Ceramic cup samplers are used for these studies and result in little soil profile disturbance. However, a limitation to using this tool is frequent sampler failure caused by frequent system air leaks. Also, conventional installation and sampling require that samplers be accessible from above the soil line. This imposes a constraint on turf maintenance and increases traffic and wear to turf plots. Herein, an inexpensive offsite soil-water sampling method using permanently installed ceramic cup samplers that allows for routine turf maintenance without system failure is described. Thirty-six separately irrigated 4 m2 plots each with an installed sampler provided daily data over 1988-1989, from which the effects of a range of irrigation, N, K, and propoxur treatments on soil-water concentrations were evaluated. These data, plus calculated percolation provided an estimate of groundwater loading.


2020 ◽  
Vol 16 (2) ◽  
pp. 245
Author(s):  
Febrian Sayow ◽  
Bobby Vian Jhon Polii ◽  
Wenny Tilaar ◽  
Kojoh Deanne Augustine

This study aims to (1). Knowing the content and quality of Rahayu's tofu and tempe factory liquidwaste in Uner Village, Kawangkoan Sub-district, Minahasa Regency, and (2). To find out whether Rahayu's tofu and tempe factory liquid waste has exceeded the quality standard. This research was conducted for 3 months, namely in January 2020- March 2020. Sampling locations were carried out in the factory of Tahu and Tempe Rahayu Sub-district in Uner Village, Kawangkoan District, then continued with the analysis at the Manado Industrial Research and Standardization Laboratory (Baristand). This research was a descriptive research and laboratory analysis. Waste water sampling was carried out using a composite sampling method. Sampling was done in the first two places, raw water is used to make tofu and tempe in an inlet and the first was taken from the tofu factory wastewater and tempe in the sewer. Waste liquid sampling was carried out 3 times and for analysis carried out at the Baristand Laboratory to obtain data according to the parameters set in this study. The results showed that the quality of Rahayu tofu and tempe industrial liquid waste based on the analysis of BOD, COD, and TSS parameters that have been analyzed accordingly and have not passed the quality standards set by the Government. However, for the pH parameters are not appropriate and in accordance with the quality standards set by Government.*eprm*


2020 ◽  
Author(s):  
Edina YQ Tan ◽  
Russell RE Wee ◽  
Young Ern Saw ◽  
Kylie JQ Heng ◽  
Joseph WE Chin ◽  
...  

During a crisis, the messaging platform WhatsApp allows crisis-related information to be disseminated quickly. Although case studies have documented how WhatsApp has shaped crisis outcomes in both beneficial and harmful ways, little is known about: (i) how crisis-related content is spread; (ii) characteristics of users based on usage patterns; or (iii) how usage patterns link to well-being. During the coronavirus disease 2019 (COVID-19) crisis, this study used the experience sampling method to track the daily WhatsApp usage of 151 adults throughout one week (capturing a total of 924 days of crisis-related communication). Each day, participants reported the extent to which they had received, forwarded, or discussed COVID-19- related content. During the week-long monitoring, most participants (94.7%) reported at least one COVID-19 related use of WhatsApp. Those who engaged with more COVID-19 content in personal chats were more likely to report having COVID-19 thoughts throughout the day. We further observed that around 1 in 10 individuals (14%) were chronic users who received and shared forwarded COVID-19 messages at a high volume; this group may represent everyday "super spreaders" of crisis-related content. Together, these findings provide an empirical base for policy makers to manage risk communication during large-scale crises.


2020 ◽  
Author(s):  
Maria Luisa Baiño Salingay ◽  
Daniel Giesen ◽  
Chris Zevenbergen

Abstract Monitoring organic pesticides in surface water using grab water sampling method is ineffective in most cases due to pesticides’ irregular emission and low solubility in water. To address the problem in water sampling method, this study used passive samplers (PS) composed of silicon rubber sheets (SR) and speedisk (SD). SR and SD were used respectively to measure hydrophobic and hydrophilic organic pesticides. These were submerged in river water uninterruptedly for 34 days. Pesticides in PS were extracted and analysed by sensitive analytical instruments HPLC, GC-MS MS and LC-MS MS. Pesticide assessment were conducted in two river systems in Cagayan de Oro River Basin, Philippines where agricultural run-off are unintentionally and continuously disposed from medium to huge plantations. The samplers were able to measure 105 emerging organic pollutants of which, 56 were organic pesticides and 22 organochlorine pesticides (OCP). Out of 56 pesticides, 11 were measured beyond the applied threshold level of 10ng/L. These were boscalid, carbendazim, dimethomorph, metalaxyl, propiconazole, pyrimethanil, diuron, monuron, simazine, dichlorvos and phenamiphos. The 22 OCP, despite being banned or restricted for almost two decades, were measured in ultra-traces of ng-pg/L. The data obtained in this study can be used to establish a baseline background level which is currently absent in most river systems in the Philippines. It is recommended that further study will be conducted using software PBPK or NORMTOX to assess health impacts and implications of the measured pesticides on the communities that are dependent, and continuously exposed to these constituents by these river waters.


Radiocarbon ◽  
2010 ◽  
Vol 52 (3) ◽  
pp. 1141-1149
Author(s):  
R Janovics ◽  
M Molnár ◽  
I Futó ◽  
L Rinyu ◽  
É Svingor ◽  
...  

An automatic water sampling unit was developed to monitor the radioactive emission (radiocarbon and other corrosion and fission products) from nuclear facilities into the groundwater. Automatic sampling is based on the principal of ion exchange using built-in resin columns in the submerging samplers. In this way, even the short-term emissions can be detected. According to our experiments, the 14C activity concentrations and the δ13C values of the samples made by the ion exchange method are systematically underestimated compared to the real values. The carbonate adsorption feature of the sampling unit was studied under laboratory and field conditions. For this purpose, a test method was developed. The observed sampling efficiencies and additionally some carbon contamination for the sampling method itself have to be taken into consideration when we estimate the amount of 14C contamination introduced into the groundwater from a nuclear facility. Therefore, a correction factor should be made for the 14C anion exchange sampling. With the help of this correction, the results converge to the expected value.


2020 ◽  
Vol 3 (2) ◽  
pp. 62
Author(s):  
Shally Yanova ◽  
Kory Asi Mariana Siagian ◽  
Rizki Gusanti

This research has been conducted in July to August 2020. The purpose of this study is to find out the heavy metal content of Hg, Cd, Pb, Mn, and Fe in the Batanghari River water of Jambi Province and determine the level of contamination by referring to PP No.82 Year 2001. The research site was conducted in Aur Duri 1 and Aur Duri 2 Jambi Provinces. Research on heavy metal content in water was conducted at 6 measurement points consisting of 3 measurement points in the Aur Duri region 1 upper and lower of the river and 3 measurement points in the Aur Duri region 2 the upper and lower of the river. Determination of river water sampling point is done using purposive sampling method. Based on the results of this study, it can be concluded that the Batanghari River water of Jambi Province has been very heavily polluted with Hg and Fe metals, if referring to PP No.82 Year 2001 Class I. The content of Mn metal in Batanghari River water mostly belongs to the category of fairly polluted metal Mn.While the metal content of Cd and Pb belongs to the category is not contaminated.


2021 ◽  
Author(s):  
Edina YQ Tan ◽  
Russell RE Wee ◽  
Young Ern Saw ◽  
Kylie JQ Heng ◽  
Joseph WE Chin ◽  
...  

BACKGROUND Worldwide, social media traffic increased following the onset of the coronavirus disease (COVID-19) pandemic. Although the spread of COVID-19 content has been described for several social media platforms (e.g., Twitter, Facebook), little is known about how content is spread via private messaging platforms such as WhatsApp. OBJECTIVE In this study, we documented: (i) how WhatsApp is used to transmit COVID-19 content; (ii) the characteristics of WhatsApp users based on their usage patterns; and(iii) how usage patterns link to well-being. METHODS We used the experience sampling method to track day-to-day WhatsApp usage during the COVID-19 pandemic. For one week, participants reported each day the extent to which they had received, forwarded, or discussed COVID-19 content. The final dataset comprised of 924 data points collected from 151 participants. RESULTS During the week-long monitoring, most participants (143/151, 95%) reported at least one COVID-19-related use of WhatsApp. When a taxonomy was generated based on usage patterns, 1 in 10 participants (21/151, 14%) were found to have received and shared a high volume of forwarded COVID-19 content – akin to ‘super spreaders’ identified on other social media platforms. Finally, those who engaged with more COVID-19 content in their personal chats were more likely to report having COVID-19 thoughts throughout the day. CONCLUSIONS These findings provide a rare window into discourse on private messenger platforms. In turn, this can inform risk communication strategies during the pandemic. CLINICALTRIAL ClinicalTrials.gov NCT04367363


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