Correspondence on “Immune thrombocytopenia in 2 healthy young women after the Pfizer‐BioNTech BNT16B2b2 messenger RNA coronavirus disease 2019 vaccination”

Immune thrombocytopenia (ITP) has been widely reported as a complication of SARS-CoV-2 infection, but to our knowledge, there have been no reports on the association of the COVID-19 vaccine with thrombocytopenia. Here, we report a case of secondary ITP in a patient who was recently immunised with the messenger RNA COVID-19 vaccine BNT162b2 (Pfizer–BioNTech).

Download Full-text

Exercise Does Not Influence Myostatin and Follistatin Messenger RNA Expression in Young Women

The Journal of Strength and Conditioning Research ◽

10.1519/jsc.0b013e3181c8664f ◽

2010 ◽

Vol 24 (2) ◽

pp. 522-530 ◽

Cited By ~ 17

Author(s):

Nicole E Jensky ◽

Jennifer K Sims ◽

Christina M Dieli-Conwright ◽

Fred R Sattler ◽

Judd C Rice ◽

...

Keyword(s):

Young Women ◽

Messenger Rna ◽

Rna Expression

Download Full-text

Identification and Validation of Gene Expression Pattern and Signature in Patients with Immune Thrombocytopenia

SLAS DISCOVERY Advancing Life Sciences ◽

10.1177/1087057116664029 ◽

2016 ◽

Vol 22 (2) ◽

pp. 187-195 ◽

Cited By ~ 1

Author(s):

Qi-dong Ye ◽

Hui Jiang ◽

Xue-lian Liao ◽

Kai Chen ◽

Shan-shan Li

Keyword(s):

Gene Expression ◽

Messenger Rna ◽

Immune Thrombocytopenia ◽

Type I Diabetes ◽

Gene Expression Pattern ◽

Enrichment Analysis ◽

Interferon Γ ◽

Gene Set Enrichment Analysis ◽

Type I ◽

Healthy Humans

In the present study, we sought to define genes associated with immune thrombocytopenia (ITP). Microarray analysis revealed that of 1002 genes associated with ITP, 309 genes had downregulated expression and 693 genes had upregulated expression in patients with ITP. Gene set enrichment analysis revealed that 11 pathways were positively correlated to ITP, such as type I diabetes mellitus, intestinal immune network for IgA production, and oxidative phosphorylation. The messenger RNA expression levels of the indicated genes, including HLA-DRB5, IGHV3-66, IFI27, FAM212A, PLD5, tumor necrosis factor (TNF)–α, interferon-γ, interleukin (IL)–1β, and IL-4, were significantly increased in patients with ITP compared with healthy humans, while MMP8, SLC1A3, CRISP3, THBS1, FMN1, and IL-10 were decreased. In conclusion, the gene expression profile of patients with ITP has established a foundation to study the gene mechanism of ITP progression.

Download Full-text

Dysregulated megakaryocyte distribution associated with nestin+ mesenchymal stem cells in immune thrombocytopenia

Blood Advances ◽

10.1182/bloodadvances.2018026690 ◽

2019 ◽

Vol 3 (9) ◽

pp. 1416-1428 ◽

Cited By ~ 2

Author(s):

Min Wang ◽

Ru Feng ◽

Jia-min Zhang ◽

Lin-lin Xu ◽

Fei-er Feng ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Messenger Rna ◽

Immune Thrombocytopenia ◽

Sympathetic Nerves ◽

Enzyme Linked Immunosorbent Assay ◽

Vegf Receptor ◽

Platelet Production ◽

Bm Niche ◽

Healthy Donors

AbstractImpaired megakaryocyte (MK) maturation and reduced platelet production are important causes of immune thrombocytopenia (ITP). However, MK distribution and bone marrow (BM) niche alteration in ITP are unclear. To investigate the maturation and distribution of MKs in the BM niche and examine the components of BM niche regulation of MK migration, BM and peripheral blood were obtained from 30 ITP patients and 28 healthy donors. Nestin+ mesenchymal stem cells (MSCs) and CD41+ MKs were sorted by fluorescence-activated cell sorting. The components of the BM niche and related signaling were analyzed via immunofluorescence, flow cytometry, enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and western blot analysis. The number of MKs in the BM vascular niche was reduced in ITP. Moreover, the concentrations of CXCL12 and CXCR4+ MKs in the BM were decreased in ITP. Further investigation demonstrated that nestin+ MSCs and CXCL12 messenger RNA (mRNA) in nestin+ MSCs were both reduced whereas the apoptosis of nestin+ MSCs was significantly increased in ITP. Sympathetic nerves, Schwann cells, the proportion of β3-adrenoreceptor (β3-AR)+ nestin+ MSCs, and β3-AR mRNA in nestin+ MSCs were all markedly reduced in ITP. Moreover, matrix metalloproteinase 9, vascular endothelial growth factor (VEGF), and VEGF receptor 1 were significantly reduced in ITP. Our data show that impaired MK distribution mediated by an abnormal CXCL12/CXCR4 axis is partially involved in reduced platelet production in ITP. Moreover, sympathetic neuropathy and nestin+ MSC apoptosis may have an effect on the alterations of BM CXCL12 in ITP.

Download Full-text

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166944 ◽

1996 ◽

Vol 54 ◽

pp. 896-897

Author(s):

G. W. Hacker ◽

I. Zehbe ◽

J. Hainfeld ◽

A.-H. Graf ◽

C. Hauser-Kronberger ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Messenger Rna ◽

High Performance ◽

Detection System ◽

Direct Methods ◽

Genetic Alterations ◽

Chain Reaction ◽

Protein Encoding ◽

Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

Download Full-text

Severe Paradoxical Dysphonia in Two Young Women

American Journal of Speech-Language Pathology ◽

10.1044/1058-0360.0203.52 ◽

1993 ◽

Vol 2 (3) ◽

pp. 52-55 ◽

Cited By ~ 1

Author(s):

Michael Collins ◽

Robert McDonald ◽

Robert Stanley ◽

Timothy Donovan ◽

C. Frank Bonebrake

Keyword(s):

Young Women ◽

Theoretical Basis ◽

Therapeutic Regimen ◽

Instrumental Data ◽

Relationship Of ◽

The Relationship

This report describes an unusual and persistent dysphonia in two young women who had taken a therapeutic regimen of isotretinoin for intractable acne. We report perceptual and instrumental data for their dysphonia, and pose a theoretical basis for the relationship of dysphonia to this drug. We also provide recommendations for reducing the risk of acquiring a dysphonia during the course of treatment with isotretinoin.

Download Full-text

What Do We Want Them To Be When They Grow Up?

Perspectives on Hearing and Hearing Disorders in Childhood ◽

10.1044/hhdc20.1.27 ◽

2010 ◽

Vol 20 (1) ◽

pp. 27-31

Author(s):

Lyn Robertson

Keyword(s):

Hearing Loss ◽

Social Development ◽

Young Women ◽

Hearing Aids ◽

Test Scores ◽

Spoken Language ◽

Language Therapy ◽

Profound Hearing Loss ◽

Children With Hearing Loss ◽

Learning To Listen

Abstract Learning to listen and speak are well-established preludes for reading, writing, and succeeding in mainstream educational settings. Intangibles beyond the ubiquitous test scores that typically serve as markers for progress in children with hearing loss are embedded in descriptions of the educational and social development of four young women. All were diagnosed with severe-to-profound or profound hearing loss as toddlers, and all were fitted with hearing aids and given listening and spoken language therapy. Compiling stories across the life span provides insights into what we can be doing in the lives of young children with hearing loss.

Download Full-text