scholarly journals Unidirectional and sustained delivery of the proresolving lipid mediator resolvin D1 from a biodegradable thin film device

2016 ◽  
Vol 105 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Kevin D. Lance ◽  
Anuran Chatterjee ◽  
Bian Wu ◽  
Giorgio Mottola ◽  
Harald Nuhn ◽  
...  
Keyword(s):  
Thin Film ◽  
Lipid Mediator ◽  
Sustained Delivery ◽  
Resolvin D1 ◽  
Thin Film Device

Influence of Shape Change Caused by Warp of Thin-film Device on Solar Radiation Pressure Torque in Solar Sail

2020 ◽  
Vol 19 (0) ◽  
pp. 101-110
Author(s):  
Rikushi KATO ◽  
Masanori MATSUSHITA ◽  
Hideyuki TAKAHASHI ◽  
Osamu MORI ◽  
Nobukatsu OKUIZUMI ◽  
...  
Keyword(s):  
Thin Film ◽  
Solar Radiation ◽  
Radiation Pressure ◽  
Shape Change ◽  
Solar Radiation Pressure ◽  
Solar Sail ◽  
Thin Film Device

Effect of Curved Thin-film Device on Natural Frequency of Rectangle Membrane under Uniaxial Tension

2019 ◽  
Vol 18 (0) ◽  
pp. 73-80 ◽  
Author(s):  
Masanori MATSUSHITA ◽  
Nobukatsu OKUIZUMI ◽  
Yasutaka SATOU ◽  
Osamu MORI ◽  
Takashi IWASA ◽  
...  
Keyword(s):  
Thin Film ◽  
Uniaxial Tension ◽  
Natural Frequency ◽  
Thin Film Device

Thermal expansion of LaAlO3 and (La,Sr)(Al,Ta)O3, substrate materials for superconducting thin-film device applications

1998 ◽  
Vol 83 (4) ◽  
pp. 1979-1982 ◽  
Author(s):  
B. C. Chakoumakos ◽  
D. G. Schlom ◽  
M. Urbanik ◽  
J. Luine
Keyword(s):  
Thin Film ◽  
Thermal Expansion ◽  
Superconducting Thin Film ◽  
Thin Film Device ◽  
Device Applications

Capacitance Measurement in Organic Thin-Film Device with Internal Charge Separation Zone

2005 ◽  
Vol 44 (No. 33) ◽  
pp. L1059-L1062 ◽  
Author(s):  
Masaya Terai ◽  
Katsuhiko Fujita ◽  
Tetsuo Tsutsui
Keyword(s):  
Thin Film ◽  
Charge Separation ◽  
Separation Zone ◽  
Capacitance Measurement ◽  
Organic Thin Film ◽  
Thin Film Device ◽  
Internal Charge

Magnetoresistance effect of Ga-Sn-O thin-film device

2016 ◽  
Author(s):  
Asuka Fukawa ◽  
Kota Imanishi ◽  
Shogo Miyamura ◽  
Tokiyoshi Matsuda ◽  
Mutsumi Kimura
Keyword(s):  
Thin Film ◽  
Magnetoresistance Effect ◽  
Thin Film Device

Abstract 314: Development of a Nanotube-coated Nitinol Stent for Delivery of Resolvin D1

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Phin-Peng Lee ◽  
Anuran Chatterjee ◽  
Bian Wu ◽  
Harald Nuhn ◽  
Tejal Desai ◽  
...  
Keyword(s):  
Stent Implantation ◽  
Vessel Wall ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Rabbit Aorta ◽  
Dip Coating ◽  
Lipid Mediator ◽  
Drug Eluting Stent ◽  
Resolvin D1 ◽  
Nitinol Stents

Introduction: As part of a Vascular Innovation and Therapeutic Advances (VITA) contract from NHLBI, we sought to optimize a nitinol drug eluting stent to deliver a novel bioactive lipid mediator Resolvin D1 (RvD1). Methods: Nitinol stents were secured as the anode in an ethylene glycol-based anodization setup to create nanotubes on the stent surface. Scanning electron microscopy was used to evaluate the quality of the coatings where an iterative process was utilized to optimize anodization conditions (70V, 5min). To ascertain structural integrity of the coatings, stents were observed under SEM after compression in a crimping device followed by self-expansion. Nanotube-coated (NT) and unmodified bare metal (BM) nitinol stents were loaded with RvD1 by dip-coating (with sonication) followed by hanging drop evaporation of RvD1 (in ethanol). Stents were eluted in media and RvD1 levels were analyzed by EIA. An ex vivo model of vascular stent implantation was created using a 3D printer and RvD1 delivery to the vessel wall (segment of rabbit aorta) and circulating flow medium was quantified after 3 hrs of stent implantation. Results: NT stents released significant amounts of RvD1 (1273ng cumulative at day 9), where 1177ng (± 238) of RvD1 was released within 3hr and 0.111ng (± 0.014) of RvD1 was released between day 7 and day 9. Net amount of RvD1 released was 0.2nM - 2000nM, which is physiologically relevant and within the milestone goal. NT stents released much higher levels of RvD1 (9.7 pg/mg vessel; ±1.39) to the vessel wall compared to standard BM stent controls (3.9 pg/mg vessel; ±2.0) and native vessel (1.3 pg/mg vessel; ±0.5). Conclusion: We have developed optimized anodization conditions for creating a uniform coating of titania nanotubes on nitinol stents that is able to survive crimping and self-expansion. NT stents demonstrate improved loading and elution of the lipid mediator RvD1 in an ex-vivo artery flow model.

Abstract 31: Resolvin D1 Attenuates PDGF-induced Vascular Smooth Muscle Cell Migration via the cAMP Pathway

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Giorgio Mottola ◽  
Bian Wu ◽  
Anuran Chatterjee ◽  
Mian Chen ◽  
Michael S Conte
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Neointimal Hyperplasia ◽  
Lipid Mediator ◽  
Resolvin D1 ◽  
Scratch Assay ◽  
Migratory Activity ◽  
Downstream Signaling ◽  
Rac1 Activity ◽  
Camp Levels

Introduction: Resolvin D1 (RvD1), a specialized pro-resolving lipid mediator (SPM), attenuates migration in vascular smooth muscle cells (VSMC), which is critical to the development of neointimal hyperplasia. SPM are known to interact with G-protein coupled receptors (GPCR). We sought to investigate the pathways by which RvD1 influences VSMC migration. Methods: VSMC were harvested from human saphenous veins. cAMP levels were measured via ELISA in the absence or presence of RvD1 receptor (ALX, GPR32) blockers. NF449, a G s -protein inhibitor, was also used. PDGF-BB (10ng/ml) was used as an agonist in a VSMC scratch assay as well as the receptor blockers. PDGF-BB-induced cytoskeletal changes were measured as aspect ratio after actin staining, and a scratch assay was used to assess migration. PDGF-induced Rac1 activity was measured via ELISA; VASP phosphorylation was assessed by Western blot and Paxillin translocation by Immunofluorescence. PKA inhibitor Rp-8-Br-cAMP (10μM) was used in the phenotypic and downstream signaling studies. Results: RvD1 treatment (10nM) of VSMC induced a significant acute flux in cAMP levels at 5 minutes (Fig. 1A; n≥3); this increase was abolished by an ALX antagonist (WRW4), the anti-GPR32 blocking Ab, and NF449. RvD1 (10nM) attenuated PDGF-induced VSMC migration (Fig. 1B-C; n≥3), cytoskeletal rearrangements (n=4), Rac1 activation (n≥3) and increased phosphorylation of VASP (n=3). These effects were negated by the addition of Rp-8-Br-cAMP, suggesting cAMP involvement. RvD1’s anti-migratory effect was reversed by blocking ALX or GPR32 (Fig. 1C; n≥8). Conclusion: Our results suggest that RvD1 attenuates VSMC migration by increasing levels of cAMP through ALX and GPR32 via a G s -protein-mediated action. Interference with Rac1, VASP and Paxillin function appear to be important for the anti-migratory activity of RvD1.

Sign in / Sign up

Export Citation Format

Share Document