Diagnostic value of Lipoarabinomannan antigen for detecting Mycobacterium tuberculosis in adults and children with or without HIV infection

Tuberculosis is caused by organisms of the Mycobacterium tuberculosis complex, including M. tuberculosis (the most important), M. bovis, and M. africanum. It has been present since antiquity and is the second leading infectious cause of death after HIV infection. An estimated 2 billion people worldwide carry latent infection, when ...

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Acute purulent pericarditis due co-infection with Staphylococcus aureus and Mycobacterium tuberculosis as first manifestation of HIV infection

Oxford Medical Case Reports ◽

10.1093/omcr/omy127 ◽

2019 ◽

Vol 2019 (2) ◽

Cited By ~ 2

Author(s):

Edgar Stroppa Lamas ◽

Ricardo Jose Rogoni Bononi ◽

Mario Vinicius Angelete Alvarez Bernardes ◽

Junior Luiz Pasin ◽

Hector Aurelio Davial Soriano ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Mycobacterium Tuberculosis ◽

Hiv Infection ◽

Purulent Pericarditis

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Preferential infection and depletion of Mycobacterium tuberculosis–specific CD4 T cells after HIV-1 infection

Journal of Experimental Medicine ◽

10.1084/jem.20100090 ◽

2010 ◽

Vol 207 (13) ◽

pp. 2869-2881 ◽

Cited By ~ 155

Author(s):

Christof Geldmacher ◽

Njabulo Ngwenyama ◽

Alexandra Schuetz ◽

Constantinos Petrovas ◽

Klaus Reither ◽

...

Keyword(s):

T Cells ◽

Mycobacterium Tuberculosis ◽

Hiv Infection ◽

Cd4 T Cells ◽

Staphylococcal Enterotoxin B ◽

Opportunistic Pathogens ◽

Rapid Loss ◽

Hiv 1

HIV-1 infection results in the progressive loss of CD4 T cells. In this study, we address how different pathogen-specific CD4 T cells are affected by HIV infection and the cellular parameters involved. We found striking differences in the depletion rates between CD4 T cells to two common opportunistic pathogens, cytomegalovirus (CMV) and Mycobacterium tuberculosis (MTB). CMV-specific CD4 T cells persisted after HIV infection, whereas MTB-specific CD4 T cells were depleted rapidly. CMV-specific CD4 T cells expressed a mature phenotype and produced very little IL-2, but large amounts of MIP-1β. In contrast, MTB-specific CD4 T cells were less mature, and most produced IL-2 but not MIP-1β. Staphylococcal enterotoxin B–stimulated IL-2–producing cells were more susceptible to HIV infection in vitro than MIP-1β–producing cells. Moreover, IL-2 production was associated with expression of CD25, and neutralization of IL-2 completely abrogated productive HIV infection in vitro. HIV DNA was found to be most abundant in IL-2–producing cells, and least abundant in MIP-1β–producing MTB-specific CD4 T cells from HIV-infected subjects with active tuberculosis. These data support the hypothesis that differences in function affect the susceptibility of pathogen-specific CD4 T cells to HIV infection and depletion in vivo, providing a potential mechanism to explain the rapid loss of MTB-specific CD4 T cells after HIV infection.

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Diagnostic value of antibody responses to multiple antigens from Mycobacterium tuberculosis in active and latent tuberculosis

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2015.07.021 ◽

2015 ◽

Vol 83 (3) ◽

pp. 278-285 ◽

Cited By ~ 12

Author(s):

Muhammad Andrian Senoputra ◽

Beata Shiratori ◽

Fakhrial Mirwan Hasibuan ◽

Raspati Cundarani Koesoemadinata ◽

Lika Apriani ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Latent Tuberculosis ◽

Diagnostic Value ◽

Antibody Responses

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IMMUNOMORPHOLOGY OF PARADOXICAL TUBERCULOSIS WITH CONCURRENT HIV INFECTION AND IRIS, CLINICAL AND DIAGNOSTIC VALUE

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2018-96-5-51-52 ◽

2018 ◽

Vol 96 (5) ◽

pp. 51-52

Author(s):

L. S. Bykhalov ◽

A. V. Smirnov

Keyword(s):

Hiv Infection ◽

Diagnostic Value

.

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The Value of Metagenomic Next -Generation Sequence In Acquired Immunodeficiency Syndrome With Opportunistic Infections

10.21203/rs.3.rs-728309/v1 ◽

2021 ◽

Author(s):

Li Liu ◽

Mingjuan Yuan ◽

Siqing Sun ◽

Jinrong Wang ◽

Yi Shi ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Acquired Immunodeficiency Syndrome ◽

Opportunistic Infections ◽

Treatment Strategies ◽

Diagnostic Value ◽

Antimicrobial Treatment ◽

Next Generation ◽

Antibiotic Drugs ◽

Acquired Immunodeficiency ◽

Immunodeficiency Syndrome

Abstract Background: Metagenomic next-generation sequence (mNGS) is an emerging powerful pan-pathogen test for the diagnosis of infectious diseases. However, the application of mNGS in acquired immunodeficiency syndrome with opportunistic infections is limited to clinical cases reports and central nervous system infection.In this study, we evaluated the diagnostic value of mNGS in acquired immunodeficiency syndrome(AIDS) with opportunistic infections(OIs).Methods: From January 2018 to February 2021,86 cases were enrolled in this retrospective analysis. All patients underwent mNGS. Clinical data were recorded.Result: In the present study, mNGS identified 76 of 86 infection cases (88.37%).Human betaherpesvirus 5 (CMV) (40.70%), Human gammaherpesvirus 4 (EBV) (40.70%),pneumocystis (31.40%) were the most common pathogens detected. The sensitivity of mNGS (88.37%,76/86) was higher than that of culture (22.10%, 19/86),smear(7%,6/86) and PCR(46.51%,40/86).In the detection of viruses such as (CMV and EBV), the consistency between PCR and mNGS of CMV and EBV was 100%,73.33% respectively. All PCP cases were detected by mNGS. The consistency in detection of talaromyces between culture and mNGS was 75%.mNGS is superior to the common methods such as culture and smear in the detection of mycobacterium tuberculosis and non-mycobacterium tuberculosis. The mNGS findings led to changes in treatment strategies in 47/86 (54.65%) cases. Compared with the patients’treatment before mNGS, patients had lower rate of broad-spectrum antibiotic drugs use during clinical treatment after mNGS 78/86(90.70%) vs 34/86(39.53%)（P<0.0001). Conclusion: mNGS showed a satisfying diagnostic performance in acquired immunodeficiency syndrome with opportunistic infections. mNGS may lead to a more precise antimicrobial treatment and reduced the use of antibiotic medicine.

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Diagnostic value of serum IgG by Eliza to detecting Mycobacterium tuberculosis, Original Article

Journal of Research in Clinical Medicine ◽

10.34172/jrcm.2021.029 ◽

2021 ◽

Vol 9 (1) ◽

pp. 29-29

Author(s):

Mehdi Haghdoost ◽

Parisa Alizadeh Nazmi ◽

Hamid Owaysee Osquee

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Immunoglobulin G ◽

Diagnostic Value ◽

World Health ◽

Control Group ◽

Reference Laboratory ◽

Predictive Values ◽

Serum Igg ◽

Significant Difference

Introduction: In developing countries, which is an endemic region in terms of tuberculosis, there is an urgent need for fast, accurate, and inexpensive serological testing. The aim of this study was to determine the diagnostic value of patient serum IgG antibodies by ELISA in the diagnosis of Mycobacterium tuberculosis. Method: This case-control study was performed on patients with pulmonary tuberculosis in 2017-2020. After selecting the case (n = 30) and control (n = 30) subjects according to inclusion criteria, their blood samples were obtained and analyzed in the reference laboratory by standard kits for immunoglobulin G against 16, 36, and 40 kDa antigens of mycobacterium tuberculosis. Results: The mean age of the subjects was 47.07 (15.57%). The majority of participants were 46 (51.1%) women. There was no significant difference between the two groups regarding sex and age. serological examination of patients with pulmonary tuberculosis showed 25 positive results and only 4 of the control group had a positive result. Sensitivity, specificity, positive and negative predictive values of serology test were 83.3%, 86.67%, 86.20% and 87.88% respectively. Conclusion: Despite the acceptable sensitivity of the serologic immunoglobulin G test, according to the statement of World health organization (WHO), it did not possess an acceptable specificity. It is recommended that a a wider range of different antigens to be studied also it is essential to evaluate the diagnostic value of the other immunoglobulins inpatient in different stages of the disease.

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Differential Expression of Activation Markers by Mycobacterium tuberculosis-specific CD4+ T Cell Distinguishes Extrapulmonary From Pulmonary Tuberculosis and Latent Infection

Clinical Infectious Diseases ◽

10.1093/cid/ciz1070 ◽

2019 ◽

Vol 71 (8) ◽

pp. 1905-1911 ◽

Cited By ~ 8

Author(s):

Paulo S Silveira-Mattos ◽

Beatriz Barreto-Duarte ◽

Beatriz Vasconcelos ◽

Kiyoshi F Fukutani ◽

Caian L Vinhaes ◽

...

Keyword(s):

T Cells ◽

Mycobacterium Tuberculosis ◽

T Cell ◽

Hiv Infection ◽

Cd4 T Cells ◽

Cell Activation ◽

Cd4 T Cell ◽

Activation Markers ◽

Timely Initiation ◽

Latent Tb

Abstract Background Diagnosis of active tuberculosis (ATB) currently relies on detection of Mycobacterium tuberculosis (Mtb). Identifying patients with extrapulmonary TB (EPTB) remains challenging because microbiological confirmation is often not possible. Highly accurate blood-based tests could improve diagnosis of both EPTB and pulmonary TB (PTB) and timely initiation of anti-TB therapy. Methods A case-control study was performed using discriminant analyses to validate an approach using Mtb-specific CD4+T-cell activation markers in blood to discriminate PTB and EPTB from latent TB infection (LTBI) as well as EPTB from PTB in 270 Brazilian individuals. We further tested the effect of human immunodeficiency virus (HIV) coinfection on diagnostic performance. Frequencies of interferon-γ +CD4+T cells expressing CD38, HLADR, and/or Ki67 were assessed by flow cytometry. Results EPTB and PTB were associated with higher frequencies of CD4+T cells expressing CD38, HLADR, or Ki67 compared with LTBI (all P values < .001). Moreover, frequencies of HLADR+ (P = .03) or Ki67+ (P < .001) cells accurately distinguished EPTB from PTB. HIV infection did not affect the capacity of these markers to distinguish ATB from LTBI or EPTB from PTB. Conclusions Cell activation markers in Mtb-specific CD4+T cells distinguished ATB from LTBI and EPTB from PTB, regardless of HIV infection status. These parameters provide an attractive approach for developing blood-based diagnostic tests for both active and latent TB.

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