Can you trust your cryostat? Reproducibility of cryostat section thickness

2006 ◽  
Vol 69 (10) ◽  
pp. 835-838 ◽  
Author(s):  
Philip C. De Witt Hamer ◽  
Fonnet E. Bleeker ◽  
Aeilko H. Zwinderman ◽  
Cornelis J.F. Van Noorden
Keyword(s):  
Cryostat Section ◽  
Section Thickness

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

NICKEL-COPPER-COLUMBIUM STEEL

Alloy Digest ◽  
1971 ◽  
Vol 20 (4) ◽  
Keyword(s):  
Fracture Toughness ◽  
Corrosion Resistance ◽  
Low Temperature ◽  
Heat Treating ◽  
Section Thickness ◽  
Aged Condition ◽  
International Nickel Company ◽  
Temperature Performance ◽  
Nickel Copper ◽  
Hot Rolled

Abstract Nickel-Copper-Columbium Steel is used in the hot-rolled and aged condition and develops yield strengths in the range 75,000 to 100,000 psi, depending on alloy content and section thickness. This datasheet provides information on composition, physical properties, elasticity, and tensile properties as well as fracture toughness. It also includes information on low temperature performance and corrosion resistance as well as forming, heat treating, machining, and joining. Filing Code: SA-263. Producer or source: International Nickel Company Inc..

Glass Formation and Glass Forming Ability of Alloys Near Eutectic Composition

2000 ◽  
Vol 644 ◽  
Author(s):  
Y. Li
Keyword(s):  
Glass Transition ◽  
Glass Formation ◽  
Eutectic Composition ◽  
Glass Forming Ability ◽  
Critical Section ◽  
Bulk Glass ◽  
Melting Behaviour ◽  
Eutectic Alloys ◽  
Section Thickness ◽  
Glass Forming

AbstractOnset temperature, Tm and offset temperature (liquidus) Tl of melting of a series of bulk glass forming alloys based on La, Mg, and Pd have been measured by studying systematically the melting behaviour of these alloys using DTA or DSC. Bulk metallic glass formation has been found to be most effective at or near their eutectic points and less effective for off-eutectic alloys. Reduced glass transition temperature Trg given by Tg/Tl is found to show a stronger correlation with critical cooling rate or critical section thickness for glass formation than Trg given by Tg/Tm.

scholarly journals Elimination of autofluorescence in immunofluorescence microscopy with digital image processing.

1995 ◽  
Vol 43 (7) ◽  
pp. 727-730 ◽  
Author(s):  
C H Van de Lest ◽  
E M Versteeg ◽  
J H Veerkamp ◽  
T H Van Kuppevelt
Keyword(s):  
Image Processing ◽  
Digital Image Processing ◽  
Human Heart ◽  
Immunofluorescence Microscopy ◽  
Human Lung ◽  
Emission Spectra ◽  
Fluorescence Signal ◽  
Section Thickness ◽  
Disturbing Factor ◽  
Total Fluorescence

Autofluorescence can be a very disturbing factor in immunofluorescence microscopy. We present here a method to eliminate autofluorescence. The method is based on the fact that most autofluorescent compounds have broad-banded excitation and emission spectra, whereas specific fluorescent probes have narrow spectra. Two images are recorded and digitized, one at a wavelength exciting both the fluorescent probe and the autofluorescent molecules, and one at a wavelength exciting only the latter. Subtraction of the autofluorescence signal from the total fluorescence signal, using a self-developed computer program, results in an autofluorescence-free image. The procedure is demonstrated for elimination of elastin-derived autofluorescence in human lung alveoli and for elimination of lipofuscin-derived autofluorescence in human heart muscle. The autofluorescence signal is positively correlated with tissue section thickness (r = 0.93; p < 0.0001), and can be used to correct the specific fluorescence signals for section thickness.

Section plane selection influences the results of histomorphometric studies: the example of dental implants

2012 ◽  
Vol 57 (5) ◽  
Author(s):  
Sigmar Kopp ◽  
Mareike Warkentin ◽  
Ferenc Öri ◽  
Peter Ottl ◽  
Günther Kundt ◽  
...  
Keyword(s):  
Section Plane ◽  
Section Thickness ◽  
Implant Surface ◽  
Bone Implant ◽  
Osseointegrated Implants ◽  
Section Technique ◽  
Significant Difference ◽  
Bone To Implant Contact ◽  
The Mean ◽  
Dependent Samples

AbstractThis study was designed to determine and statistically analyze bone-to-implant contact (BIC) values for human specimens segmented in at least two different locations.Samples of human bone with fractured osseointegrated implants were obtained from six patients. Sections were prepared, dehydrated, and resin infiltrated. Undecalcified bone sections were produced using the thin-section technique according to Donath, ultimately obtaining a section thickness of approximately 20 μm. Fifteen specimens were available for histomorphometry. The bone sections were digitized and analyzed. The bone-to-metal contact (BMC) parameter was determined histomorphometrically. The BMC was returned in terms of the visibly bone-covered implant surfaces as a percentage of the total implant surface shown.The values obtained for the six implants were arranged as six maximum-distance pairs and tested for significance using the t-test for dependent samples. The mean difference in BIC was 11.69±9.79%. The two-sided test showed a significant difference (p=0.033).The accidental or deliberate choice of section plane for a bone-implant block has an influence on the BIC value. To make BIC values more comparable, a standardization of section planes is desirable.

scholarly journals CORRECTION OF POLYRIBOSOME DISTRIBUTIONS AS OBSERVED IN CELL SECTIONS BY ELECTRON MICROSCOPY

1964 ◽  
Vol 22 (3) ◽  
pp. 613-621 ◽  
Author(s):  
William Perl
Keyword(s):  
Electron Microscopy ◽  
Probability Method ◽  
Section Thickness ◽  
Thin Sections ◽  
Comparison Of The Results ◽  
Good Agreement ◽  
Rabbit Reticulocytes

Clusters of ribosomes observed by electron microscopy in thin sections of rabbit reticulocytes are of the same order of size as the section thickness of 600 A. Many of the observed clusters must therefore have been transected by the section surfaces and observed as clusters containing fewer ribosomes. A probability method of correcting for this effect is given. Comparison of the results with grid observations of ribosome distributions indicates sufficiently good agreement for application to cell section observations.

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