Jasmonic acid and salicylic acid induce the accumulation of sucrose and increase resistance to chilling injury in peach fruit

This study aimed at investigating the influence of different postharvest treatments with oxalic acid (OA) and salicylic acid (SA) on quality attributes and postharvest shelf life of temperate grown apricot varieties stored under controlled atmosphere (CA) storage conditions. After each treatment was given, the samples were stored in CA store maintained at a temperature of 0 °C, 90 ± 5% relative humidity, 5% oxygen and 15% carbon dioxide for 30 days. Results indicated that both OA and SA treatments significantly (p ≤ 0.05) retained total soluble solids, titratable acidity, color profile, ascorbic acid content and total phenolic content of apricot varieties and had a positive effect on antioxidant activity and texture of samples compared to control. However, carotenoid content was found to be higher in control. Both the treatments reduced chilling injury index, weight loss and decay percentage of samples. Moreover, it was found that SA treatment was the most effective treatment in maintaining visual color of apricots while OA maintained fruit firmness and effectively decreased the decay percentage and chilling injury index of apricot varieties. In conclusion, it was found that both OA and SA have the potential to extend storage life of apricots and maintain quality attributes of the crop during CA storage.

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The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses

The Plant Cell ◽

10.1093/plcell/koab079 ◽

2021 ◽

Cited By ~ 1

Author(s):

Huaming He ◽

Jordi Denecker ◽

Katrien Van Der Kelen ◽

Patrick Willems ◽

Robin Pottie ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Salicylic Acid ◽

Jasmonic Acid ◽

Stress Responses ◽

Negative Regulator ◽

Mediator Complex ◽

Defense Gene Expression ◽

A Genome ◽

Oxidative Stress Responses

Abstract Signaling events triggered by hydrogen peroxide (H2O2) regulate plant growth and defense by orchestrating a genome-wide transcriptional reprogramming. However, the specific mechanisms that govern H2O2-dependent gene expression are still poorly understood. Here, we identify the Arabidopsis Mediator complex subunit MED8 as a regulator of H2O2 responses. The introduction of the med8 mutation in a constitutive oxidative stress genetic background (catalase-deficient, cat2) was associated with enhanced activation of the salicylic acid pathway and accelerated cell death. Interestingly, med8 seedlings were more tolerant to oxidative stress generated by the herbicide methyl viologen (MV) and exhibited transcriptional hyperactivation of defense signaling, in particular salicylic acid- and jasmonic acid-related pathways. The med8-triggered tolerance to MV was manipulated by the introduction of secondary mutations in salicylic acid and jasmonic acid pathways. In addition, analysis of the Mediator interactome revealed interactions with components involved in mRNA processing and microRNA biogenesis, hence expanding the role of Mediator beyond transcription. Notably, MED8 interacted with the transcriptional regulator NEGATIVE ON TATA-LESS, NOT2, to control the expression of H2O2-inducible genes and stress responses. Our work establishes MED8 as a component regulating oxidative stress responses and demonstrates that it acts as a negative regulator of H2O2-driven activation of defense gene expression.

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Transcriptome Analysis of Pre-Storage 1-MCP and High CO2-Treated ‘Madoka’ Peach Fruit Explains the Reduction in Chilling Injury and Improvement of Storage Period by Delaying Ripening

International Journal of Molecular Sciences ◽

10.3390/ijms22094437 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4437

Author(s):

Han Ryul Choi ◽

Min Jae Jeong ◽

Min Woo Baek ◽

Jong Hang Choi ◽

Hee Cheol Lee ◽

...

Keyword(s):

Cold Storage ◽

Transcriptome Analysis ◽

Molecular Mechanisms ◽

Chilling Injury ◽

Chemical Properties ◽

Storage Period ◽

Differentially Expressed ◽

Peach Fruit ◽

High Co2 ◽

Physico Chemical

Cold storage of peach fruit at low temperatures may induce chilling injury (CI). Pre-storage 1-MCP and high CO2 treatments were reported among the methods to ameliorate CI and reduce softening of peach fruit. However, molecular data indicating the changes associated with pre-storage 1-MCP and high CO2 treatments during cold storage of peach fruit are insufficient. In this study, a comparative analysis of the difference in gene expression and physico-chemical properties of fruit at commercial harvest vs. stored fruit for 12 days at 0 °C (cold-stored (CS), pre-storage 1-MCP+CS, and pre-storage high CO2+CS) were used to evaluate the variation among treatments. Several genes were differentially expressed in 1-MCP+CS- and CO2+CS-treated fruits as compared to CS. Moreover, the physico-chemical and sensory data indicated that 1-MCP+CS and CO2+CS suppressed CI and delayed ripening than the CS, which could lead to a longer storage period. We also identified the list of genes that were expressed commonly and exclusively in the fruit treated by 1-MCP+CS and CO2+CS and compared them to the fruit quality parameters. An attempt was also made to identify and categorize genes related to softening, physiological changes, and other ripening-related changes. Furthermore, the transcript levels of 12 selected representative genes from the differentially expressed genes (DEGs) in the transcriptome analysis were confirmed via quantitative real-time PCR (qRT-PCR). These results add information on the molecular mechanisms of the pre-storage treatments during cold storage of peach fruit. Understanding the genetic response of susceptible cultivars such as ‘Madoka’ to CI-reducing pre-storage treatments would help breeders release CI-resistant cultivars and could help postharvest technologists to develop more CI-reducing technologies.

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Biocontrol of gray mold decay in peach fruit by integration of antagonistic yeast with salicylic acid and their effects on postharvest quality parameters

Biological Control ◽

10.1016/j.biocontrol.2008.06.012 ◽

2008 ◽

Vol 47 (1) ◽

pp. 60-65 ◽

Cited By ~ 32

Author(s):

Hongyin Zhang ◽

Longchuan Ma ◽

Lei Wang ◽

Song Jiang ◽

Ying Dong ◽

...

Keyword(s):

Salicylic Acid ◽

Gray Mold ◽

Quality Parameters ◽

Postharvest Quality ◽

Peach Fruit ◽

Antagonistic Yeast

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Thaumatin-like proteins and their possible role in protection against chilling injury in peach fruit

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2010.03.009 ◽

2010 ◽

Vol 57 (2) ◽

pp. 77-85 ◽

Cited By ~ 24

Author(s):

Anurag Dagar ◽

Haya Friedman ◽

Susan Lurie

Keyword(s):

Chilling Injury ◽

Peach Fruit

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Chrysolina herbaceamodulates jasmonic acid,cis-(+)-12-oxophytodienoic acid, (3R,7S)-jasmonoyl-l-isoleucine, and salicylic acid of local and systemic leaves in the host plantMentha aquatica

Journal of Plant Interactions ◽

10.1080/17429145.2010.545282 ◽

2011 ◽

Vol 6 (2-3) ◽

pp. 99-101 ◽

Cited By ~ 7

Author(s):

Andrea Occhipinti ◽

Simon Atsbaha Zebelo ◽

Andrea Capuzzo ◽

Massimo Maffei ◽

Giorgio Gnavi

Keyword(s):

Salicylic Acid ◽

Jasmonic Acid

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Restoration of Defective Cross Talk in ssi2 Mutants: Role of Salicylic Acid, Jasmonic Acid, and Fatty Acids in SSI2-Mediated Signaling

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2003.16.11.1022 ◽

2003 ◽

Vol 16 (11) ◽

pp. 1022-1029 ◽

Cited By ~ 42

Author(s):

Pradeep Kachroo ◽

Aardra Kachroo ◽

Ludmila Lapchyk ◽

David Hildebrand ◽

Daniel F. Klessig

Keyword(s):

Cell Death ◽

Salicylic Acid ◽

Jasmonic Acid ◽

Cross Talk ◽

Pr Genes ◽

Exogenous Application ◽

Basal Level Expression ◽

Sa Signaling ◽

Spontaneous Cell Death

The Arabidopsis mutants ssi2 and fab2 are defective in stearoyl ACP desaturase, which causes altered salicylic acid (SA)- and jasmonic acid (JA)-mediated defense signaling. Both ssi2 and fab2 plants show spontaneous cell death, express PR genes constitutively, accumulate high levels of SA, and exhibit enhanced resistance to bacterial and oomycete pathogens. In contrast to constitutive activation of the SA pathway, ssi2 and fab2 plants are repressed in JA-mediated induction of the PDF1.2 gene, which suggests that the SSI2-mediated signaling pathway modulates cross talk between the SA and JA pathways. In this study, we have characterized two recessive nonallelic mutants in the ssi2 background, designated as rdc (restorer of defective cross talk) 2 and rdc8. Both ssi2 rdc mutants are suppressed in constitutive SA signaling, show basal level expression of PR-1 gene, and induce high levels of PDF1.2 in response to exogenous application of JA. Interestingly, while the rdc8 mutation completely abolishes spontaneous cell death in ssi2 rdc8 plants, the ssi2 rdc2 plants continue to show some albeit reduced cell death. Fatty acid (FA) analysis showed a reduction in 16:3 levels in ssi2 rdc8 plants, which suggests that this mutation may limit the flux of FAs into the pro-karyotic pathway of glycerolipid biosynthesis. Both rdc2 and rdc8 continue to accumulate high levels of 18:0, which suggests that 18:0 levels were responsible for neither constitutive SA signaling nor repression of JA-induced expression of the PDF1.2 gene in ssi2 plants. We also analyzed SA and JA responses of the fab2-derived shs1 mutant, which accumulates levels of 18:0 over 50% lower than those in the fab2 plants. Even though fab2 shs1 plants were morphologically bigger than fab2 plants, they expressed PR genes constitutively, showed HR-like cell death, and accumulated elevated levels of SA. However, unlike the ssi2 rdc plants, fab2 shs1 plants were unable to induce high levels of PDF1.2 expression in response to exogenous application of JA. Together, these results show that defective cross talk in ssi2 can be restored by second site mutations and is independent of morphological size of the plants, cell death, and elevated levels of 18:0.

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