One-step multiplex PCR method for the determination of pecan and Brazil nut allergens in food products

2011 ◽  
Vol 91 (13) ◽  
pp. 2407-2411 ◽  
Author(s):  
Zora Hubalkova ◽  
Eva Rencova
Keyword(s):  
Multiplex Pcr ◽  
Food Products ◽  
Brazil Nut ◽  
One Step ◽  
Pcr Method

A multiplex PCR method mediated by universal primers for the identification of eight meat ingredients in food products

2019 ◽  
Vol 245 (11) ◽  
pp. 2385-2392 ◽  
Author(s):  
Wanwan Liu ◽  
Jing Tao ◽  
Man Xue ◽  
Jiangang Ji ◽  
Yahan Zhang ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Food Products ◽  
Universal Primers ◽  
Pcr Method

scholarly journals Rapid Method for Species-Specific Identification and Determination of Toxigenicity of Vibrio Cholerae from Natural Aquatic Environment

1970 ◽  
Vol 1 (1) ◽  
pp. 69-75
Author(s):  
Bidhan Chakraborty ◽  
Khadiza Zaman ◽  
Md Majibur Rahman
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Serological Test ◽  
Seasonal Pattern ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Pcr Multiplex ◽  
Pcr Method ◽  
Species Specific

Cholera caused by toxigenic Vibrio cholerae is a major public health problem confronting developing countries, where outbreaks occur in a regular seasonal pattern and are particularly associated with poverty and poor sanitation. It is generally accepted that seven distinct pandemics of cholera have occurred since the onset of the first pandemic in 1817. Again Vibrio cholerae is capable of surviving in aquatic environments for extended periods and is considered as autochthonous species in estuarine and brackish waters. Therefore, the present study was designed to isolate V. cholerae from natural environmental samples subsequently identified by conventional and molecular biological techniques. A total number of 10 isolates were included randomly in this study based on their initial identification. The serotypes of the isolates were determined by serological test (slide agglutination) and the number of serotypes O1, O139 and non-O1/O139 were 3, 2 and 5 respectively which were reconfirmed by PCR method. Finally, the toxigenicity of the isolates was analyzed by multiplex PCR method and five (5) isolates were found to contain the ctx gene, the major virulence factor of V. cholerae. Key Words: Vibrio cholerae, Simplex PCR, Multiplex PCR, Serotypes, Toxigenicity.   doi:10.3329/sjps.v1i1.1811 S. J. Pharm. Sci. 1(1&2): 69-75

scholarly journals IDENTIFICATION OF ROTAVIRUS I- AND E-GENOTYPES BY MULTIPLEX PCR METHOD

2019 ◽  
Vol 64 (3) ◽  
pp. 140-144 ◽  
Author(s):  
T. A. Sashina ◽  
O. V. Morozova ◽  
N. V. Epifanova ◽  
N. A. Novikova
Keyword(s):  
Multiplex Pcr ◽  
Nucleotide Sequencing ◽  
Full Genome ◽  
Nsp4 Gene ◽  
Optimal Conditions ◽  
Pcr Method ◽  
Vp6 Gene ◽  
System Information

Introduction. In recent years the presence of reassortant rotavirus strains is increasingly mentioned in the world due to the application of the full-genome based classification system. Information on the circulation of such strains in the territory of Russia is limited. The aim of this work was the development of the approach for determination of genotypes of segments encoding VP6 (I) and NSP4 (E) to reveal reassortant strains. Material and Methods. Rotavirus-positive samples were studied by means of nucleotide sequencing and multiplex PCR. Phylogenetic analysis was conducted using the Bayesian approach. Results. Three alleles of the VP6 gene (I1-1, I2-IV, I2-VII) and seven alleles of the NSP4 gene (E1-I, E1-III, E2-VI, E2-VII, E2-X, E2-XII, E3) were detected on the base of nucleotide sequences of Nizhny Novgorod rotaviruses. Taking into account these results, the oligonucleotide primers specific to genotypes I1, I2, I3 and E1, E2, E3 were designed. Optimal conditions for multiplex PCR were chosen. The method was tested using the strains collected in Nizhny Novgorod in 2018. The diversity of I and E genotypes was determined and various combinations with G and P genotypes were identified. Discussion. G9-P[8]-I1-E1 rotaviruses were predominant (32.7 %) and G2-P[4]-I2-E2 rotaviruses were in second place (29.1 %). Strains with genotypes G4-P[8]-I1-E2, G3-P[8]-I2-E2 and G2-P[4]-I2-E1 were detected sporadically. They had genes of two rotavirus genogroups, so can be considered to be reassortant. Conclusion. The proposed approach is a useful tool for the characterization of rotaviruses in the conditions of the beginning of vaccination against rotavirus infection in Russia.

scholarly journals Novel One-Step Multiplex PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of -Thalassemia

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Raquel M. Fernández ◽  
Ana Peciña ◽  
Maria Dolores Lozano-Arana ◽  
Juan Carlos García-Lozano ◽  
Salud Borrego ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Single Gene ◽  
Genetic Diagnosis ◽  
Hla Typing ◽  
Tissue Type ◽  
Hla Matching ◽  
Single Gene Disorders ◽  
One Step ◽  
Pcr Method ◽  
Genotype A

Preimplantation genetic diagnosis (PGD) of single gene disorders, combined with HLA matching (PGD-HLA), has emerged as a tool for couples at risk of transmitting a genetic disease to select unaffected embryos of an HLA tissue type compatible with that of an existing affected child. Here, we present a novel one-step multiplex PCR to genotype a spectrum of STRs to simultaneously perform HLA typing and PGD for -thalassemia. This method is being routinely used for PGD-HLA cycles in our department, with a genotyping success rate of 100%. As an example, we present the first successful PGD-HLA typing in Spain, which resulted in the birth of a boy and subsequent successful HSC transplantation to his affected brother, who is doing well 4 years following transplantation. The advantage of our method is that it involves only a round of single PCR for multiple markers amplification (up to 10 markers within theHLAand 6 markers at the-globinloci). This strategy has allowed us to considerably reduce the optimization of the PCR method for each specific PGD-HLA family as well as the time to obtain molecular results in each cycle.

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

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