STARTER CULTURES | Uses in the Food Industry

1999 ◽  
pp. 2084-2095
Author(s):  
R.C. Wigley
Keyword(s):  
Food Industry ◽  
Starter Cultures

scholarly journals Investigation of antimicrobial activity of polyphenol-enriched extracts against probiotic lactic acid bacteria

2019 ◽  
Vol 2 (1) ◽  
pp. 67
Author(s):  
Milena Dimitrova ◽  
Galin Ivanov ◽  
Kiril Mihalev ◽  
Alexander Slavchev ◽  
Ivelina Ivanova ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Food Industry ◽  
Lactobacillus Rhamnosus ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Industrial Plant ◽  
Probiotic Lactic Acid Bacteria ◽  
By Products

The antimicrobial activity of polyphenol-enriched extracts from industrial plant by-products (strawberry and bilberry press residues and distilled rose petals) against probiotic lactic acid bacteria (Lactobacillus delbrueckii subsp. bulgaricus – S10 and S19; Lactobacillus rhamnosus – YW and S25; Lactobacillus gasseri – S20; Streptococcus thermophilus – S13 and S32) was investigated. The minimum inhibitory concentration (MIC) in most strains tested was found to be relatively high (from 6.25 mg.mL-1 to 12.50 mg.mL-1). The maximum concentration of polyphenols without inhibitory effect (MCWI) ranges from 0.390mg.mL-1 to 0.781mg.mL-1. The results obtained in the present study showed that among the tested lactic acid bacteria Lactobacillus delbrueckii subsp. bulgaricus – S19, Lactobacillus rhamnosus – YW and Streptococcus thermophilus – S13 had the best growth characteristics in polyphenol-enriched culture medium. These strains had the highest MIC and MCWI values and could be used as starter cultures for polyphenol-fortified fermented milks. Practical applications: The use of polyphenol-enriched extracts from industrial plant by-products (waste) – distilled rose petals (by-products of rose oil production) and strawberry and bilberry press residues (by-products of fruit juice production) contribute for improving the economic effect and for solving environmental problems in food industry. Development of functional fermented milks with combination of probiotic starter cultures and polyphenol extracts is current and perspective direction of food industry.

scholarly journals Novel starter cultures Virgibacillus spp. selected from grasshopper sub shrimp paste to inhibit biogenic amines accumulation

2021 ◽  
Author(s):  
Yirui Zhao ◽  
Xue Sang ◽  
Hongshun Hao ◽  
Jingran Bi ◽  
Gongliang Zhang ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Hemolytic Activity ◽  
Antibiotic Susceptibility ◽  
Food Industry ◽  
Starter Cultures ◽  
Amine Oxidases ◽  
Aquatic Products ◽  
Application Potential ◽  
Safety Assessments

Abstract Controlling the content of biogenic amines (BAs) is critical to guarantee the safety of fermented aquatic products. The degradation characteristics and application potential of amine-negative starter cultures (Virgibacillus halodenitrificans CGMCC 1.18601: G25, Virgibacillus pantothenticus CGMCC 1.18602: G38) screened from grasshopper sub shrimp paste (Gssp) were studied. The enzymes of the two strains G25 and G38 that degrade BAs were amine oxidases (AOs) located on their respective cell membranes. The conditions that promoted the AO activity of Virgibacillus spp. were NaCl concentrations 5–10%, temperature 37°C, pH 7.0 and ethanol concentrations 0–2%. Safety assessments (antibiotic susceptibility, biofilm activity and hemolytic activity) indicated that Virgibacillus spp. do not present a risk to human health, and this isolate can be confidently recommended as safe starter cultures for the food industry. Then, the two strains were cultured separately as starters and applied to the Gssp to analyze their influence on the flavor and quality of the product. As far as the bad flavors in Gssp such as sulfur-organic and sulf-chlor were concerned, the response values in the starter groups by G25 and G38 were significantly reduced by 39% and 65%, respectively. For the ability of strains to degrade BAs in Gssp, G25 degraded 11.1% of histamine, 11.3% of tyramine, 15.5% of putrescine and 4.1% of cadaverine; G38 significantly degraded 10.1% of histamine, 21.8% of tyramine, 18.1% of putrescine and 5.0% of cadaverine. These results indicated that the selected species could be used as starter cultures for the control of BA accumulation and degradation in Gssp.

scholarly journals In vitro assessment of metabolic profile of Enterococcus strains of human origin

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Ashlesha Bhagwat ◽  
Uday S. Annapure
Keyword(s):  
Metabolic Profile ◽  
Food Industry ◽  
Radical Scavenging ◽  
Starter Cultures ◽  
Bile Salt Hydrolase ◽  
Human Origin ◽  
Future Potential ◽  
In Vitro Assessment ◽  
Radical Scavenging Properties

Abstract Background In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Results Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml−1) and sunflower oil (highest yield 48 μg ml−1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. Conclusion The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.

scholarly journals Fermented Cream for Curd Fortified with Probiotic Cultures: Biotechnological Aspects

2022 ◽  
Vol 51 (4) ◽  
pp. 664-673
Author(s):  
Andrew Bukharev ◽  
Natalya Gavrilova ◽  
Olga Kriger ◽  
Natalya Chernopolskaya
Keyword(s):  
Food Industry ◽  
Functional Foods ◽  
Starter Cultures ◽  
Whey Protein Concentrate ◽  
Production Volume ◽  
Microbiological Methods ◽  
Oat Flour ◽  
Complete Protein ◽  
Positive Effect ◽  
Buckwheat Flour

Introduction. Contemporary food industry strives to increase the production volume of high-quality and biologically complete protein products. The Foodnet market also raised the demand for functional foods in Russia. The research objective was to develop a new functional curd product fortified with probiotic microflora. Study objects and methods. The study featured cow’s milk, skimmed milk, cream, whey protein concentrate Milkiland-WPC 80, pollen, glutamine, starter cultures DVS Danisco Probat 576 and Howaru Bifido ARO-1, buckwheat flour, and oat flour. The experiment included physicochemical, sensory, biochemical, and microbiological methods. Results and discussion. The milk-protein base of the curd product was produced in a GEA Westfalia KDB 30 curd separator. The research involved 15 and 20% cream with two different starter cultures. In case of 15% cream, Probat 576 Howaru Bifido appeared to be 1.66 times more active than ARO-1 Howaru Bifido, in case of 20% cream the result was even higher – 1.73 times. Probat 576 also demonstrated a better active acidity, i.e. 5.5 after three hours, which was two hours faster than ARO-1. Mathematical modeling revealed the positive effect of buckwheat and oat flour on the cream fermentation process. Oat flour (5%) was the optimal prebiotic, while buckwheat flour added its color to the final product, thus spoiling its market quality. Conclusion. The new biotechnology for a curd product fortified with probiotic cultures can expand the range of functional products for sports diet.

scholarly journals Examination of Lactic Acid Bacteria to Secretion of Bacteriocins

2014 ◽  
Vol 2 ◽  
Author(s):  
Maira Urazova ◽  
Asel Moldagulova ◽  
Sandugash Anuarbekova ◽  
Altynay Tuyakova ◽  
Gulyaim Abitaeva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Food Industry ◽  
Starter Cultures ◽  
Gut Health ◽  
E Coli ◽  
Medical Sector ◽  
Sour Cream

Introduction: Bacteriocins produced by lactic acid bacteria (LAB) have the potential to cover a very broad field of applications, including the food industry and the medical sector. In the food industry, bacteriocinogenic LAB strains can be used as starter cultures, co-cultures, and bioprotective cultures, which would be used to improve food quality and safety. In the medical sector, bacteriocins of probiotic LAB might play a role in interactions, which take place in human gastrointestinal tract, and contribute to gut health. The aim of this study was the examine the effect of LAB antimicrobial activity. Methods: LAB were isolated from different commercial and home made products, such as kazy and sour cream. To screen for bacteriocin producing LAB, we used an agar diffusion bioassay, described in a previous study by Dr. Yang, with three modifications in cell-free supernatant (CFS). First we had a clear supernatant, second we adjusted the CFS to pH 6.0 to eliminate acids antimicrobial effects, and third the CFS pH 6.0 was treated with catalase to exclude the action of H2O2 and confirm action of bacteriocin-like substances. Pathogenic S.marcescens, E. coli, S.aureus cultures were used as indicators. Results: Screening of 95 strains of LAB through deferred antagonism to six indicator cultures showed that all of the selected strains had a high value of antibacterial activity. However, CFS of only 50 strains retained their antimicrobial activity, and 10 of them lost this activity in the second modification of CFS with pH 6.0 to test culture S.marcescens, which confirmed the acidic nature of antimicrobial activity of CFS. Lb.rhamnosus (P-1), Lb.fermentum (N-6), and Lc.lactis (7M) lost antibacterial activity in the presence of the catalase. All modifications of CFS of three strains: Lb.pentosus (16al), Lb.pentosus (P-2), and Pediococcusacidilactici (8) retained inhibitory activity to E.coli and S. aureus. Supernatants of only Lactococcusgarvieae (10a) and Pediococcusacidilactici (25) extracted from homemade meat food kazy (Karaganda) and sour cream (Astana), respectively retained antibacterial activity to all three indicator cultures. Conclusion: The antibacterial activity (pH 6.0, added catalase) of Lactococcusgarvieae (10a) and Pediococcusacidilactici (25) to S. marcescens, E. coli, and S.aureus indicates these strains as promising strains for further use in the preparation of bacteriocins.

scholarly journals Antimicrobial resistance of lactobacilli isolated from food

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 27-32 ◽  
Author(s):  
M. Dušková ◽  
R. Karpíšková
Keyword(s):  
Antimicrobial Resistance ◽  
Food Industry ◽  
Meat Products ◽  
Starter Cultures ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Genus Lactobacillus ◽  
Fermented Dairy

Bacteria of the genus Lactobacillus are of great benefit in many areas of life. They are widely used in food industry, in particular as part of starter cultures for fermented dairy and meat products, but also in human and veterinary medicine as probiotics. The increasing global problem of antimicrobial resistance may also involve lactic acid bacteria because of the possible risk of resistance genes transfer. We determined the antimicrobial susceptibility of lactobacilli isolated from food. Ninety facultatively heterofermentative lactobacilli isolated from retail dairy and meat products were tested. The resistance to antimicrobials was screened by the disk diffusion method and the minimum inhibitory concentrations were determined by the broth microdilution method. Fifteen strains (17%) were resistant to at least one antimicrobial agent and one strain was multiresistant.

Knowledge landscape of starter cultures: A bibliometric and patentometric study

2021 ◽  
Vol 15 ◽  
Author(s):  
Rosmery Cruz-O'Byrne ◽  
Cristian Casallas-Useche ◽  
Nelson Piraneque-Gambasica ◽  
Sonia Aguirre-Forero
Keyword(s):  
Biological Sciences ◽  
Food Industry ◽  
Search Strategy ◽  
Starter Culture ◽  
Global Market ◽  
Starter Cultures ◽  
Industrial Processes ◽  
Fermented Foods ◽  
Exponential Behavior ◽  
Productive Author

Background: Starter cultures are essential in food industry biotechnology, consisting of microorganism preparations inoculated to produce safe fermented foods with desirable sensory characteristics. Objective: This study aims to identify and analyze the growth and flow of knowledge about starter cultures by creating scientific and technological profiles using patentometric and bibliometric indicators. Methods: A search for patents and scientific articles was conducted in December 2020 following a proposed 10-step methodology using the Scopus® and Patentinspiration databases. The search strategy was based on the keywords "starter culture" and “fermentation” considering publications up to 2020. Results: A total of 3035 articles and 719 patents were published until 2020, presenting a more significant number in the last ten (10) years due to the development of biological sciences and molecular biology with enzymes and microorganisms. Italy leads the scientific production while China leads the technological. It was also possible to determine the most productive author and inventors, the most influential articles and inventions, and the main scientific journals and patent offices. Conclusion: Scientific and technological activities have an exponential behavior showing that the knowledge about starter cultures continues to grow, becoming a field of interest for optimizing industrial processes related to food fermentation, thus achieving diversification of products that can satisfy the demand for food in an increasingly competitive global market.

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