Preparation of Lipid–Peptide–DNA (LPD) Nanoparticles and Their Use for Gene Transfection

2020 ◽  
pp. 91-98
Author(s):  
Fan Zhang ◽  
Hao-Ying Li
Keyword(s):  
Gene Transfection

The Effect of α1, 2-Fucosyl Transferase Gene Transfection on p38MAPK Signaling Pathway-mediated Apoptosis of Ovarian Carcinoma RMG-I Cells*

2010 ◽  
Vol 37 (2) ◽  
pp. 175-183 ◽  
Author(s):  
Jian-Ping CONG ◽  
Bei LIN ◽  
Juan-Juan LIU ◽  
Qing LIU ◽  
Fei-Fei LI ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Signaling Pathway ◽  
Gene Transfection ◽  
Transferase Gene ◽  
P38mapk Signaling ◽  
I Cells

Development of Quantum Dot in Gene Transfection

2013 ◽  
Vol 29 (1) ◽  
pp. 3
Author(s):  
Qiong WANG ◽  
Jienan CHEN ◽  
Lin ZHANG
Keyword(s):  
Quantum Dot ◽  
Gene Transfection

Non-Viral Vectors for Gene Delivery

2018 ◽  
Vol 9 (1) ◽  
pp. 4-11 ◽  
Author(s):  
Aparna Bansal ◽  
Himanshu
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Expression System ◽  
Target Cells ◽  
Specific Expression ◽  
Naked Dna

Introduction: Gene therapy has emerged out as a promising therapeutic pave for the treatment of genetic and acquired diseases. Gene transfection into target cells using naked DNA is a simple and safe approach which has been further improved by combining vectors or gene carriers. Both viral and non-viral approaches have achieved a milestone to establish this technique, but non-viral approaches have attained a significant attention because of their favourable properties like less immunotoxicity and biosafety, easy to produce with versatile surface modifications, etc. Literature is rich in evidences which revealed that undoubtedly, non–viral vectors have acquired a unique place in gene therapy but still there are number of challenges which are to be overcome to increase their effectiveness and prove them ideal gene vectors. Conclusion: To date, tissue specific expression, long lasting gene expression system, enhanced gene transfection efficiency has been achieved with improvement in delivery methods using non-viral vectors. This review mainly summarizes the various physical and chemical methods for gene transfer in vitro and in vivo.

scholarly journals Effect of vascular endothelial growth factor 165 gene transfection on bone defects and its mRNA expression in rabbits

2007 ◽  
Vol 120 (13) ◽  
pp. 1187-1191 ◽  
Author(s):  
Dong-mei ZHAO ◽  
Hai-bin WANG ◽  
Jia-feng YANG ◽  
Shi-qing WU ◽  
Jun-li LIU ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Mrna Expression ◽  
Gene Transfection ◽  
Bone Defects ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Enhanced gene transfection performance and biocompatibility of polyethylenimine through pseudopolyrotaxane formation with α-cyclodextrin

2017 ◽  
Vol 28 (12) ◽  
pp. 125102 ◽  
Author(s):  
Li-Zhong Hu ◽  
Ning Wan ◽  
Xi-Xi Ma ◽  
Zi-Wei Jing ◽  
Ya-Xuan Zhang ◽  
...  
Keyword(s):  
Gene Transfection

scholarly journals Biopolymeric Materials Used as Nonviral Vectors: A Review

2021 ◽  
Vol 2 (1) ◽  
pp. 100-109
Author(s):  
Jailson de Araújo Santos ◽  
Daniel Barbosa Liarte ◽  
Alessandra Braga Ribeiro ◽  
Marcia dos Santos Rizzo ◽  
Marcília Pinheiro da Costa ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Web Of Science ◽  
Gene Transfection ◽  
Genetic Material ◽  
Nonviral Vectors ◽  
Cationic Polymers ◽  
Genetic Trait ◽  
Low Diversity ◽  
Different Types ◽  
Materials Used

Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.

Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles

2021 ◽  
Vol 9 (7) ◽  
pp. 2454-2466
Author(s):  
Yingying Liu ◽  
Yuli Zhou ◽  
Jinfeng Xu ◽  
Hui Luo ◽  
Yao Zhu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Molecular Imaging ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Tumor Gene Therapy ◽  
Ultrasound Molecular Imaging ◽  
Tumor Gene

A novel dual-targeted cationic microbubbles help to improve gene transfection efficiency.

The varied influences of cell adhesion and spreading on gene transfection of mesenchymal stem cells on a micropatterned substrate

2021 ◽  
Author(s):  
Yongtao Wang ◽  
Yingjun Yang ◽  
Xinlong Wang ◽  
Naoki Kawazoe ◽  
Yingnan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Gene Transfection

Ultrasound‐Targeted Microbubble Destruction Mediates Gene Transfection for Beta‐Cell Regeneration and Glucose Regulation

Small ◽  
2021 ◽  
pp. 2008177
Author(s):  
Chao Zhang ◽  
Shuyuan Chen ◽  
Qunying Li ◽  
Jiao Wu ◽  
Fuqiang Qiu ◽  
...  
Keyword(s):  
Beta Cell ◽  
Gene Transfection ◽  
Glucose Regulation ◽  
Cell Regeneration ◽  
Beta Cell Regeneration
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