Species with Haploid or Doubled Haploid Protocols

2021 ◽  
pp. 41-103
Author(s):  
Jose M. Seguí-Simarro ◽  
Javier Belinchón Moreno ◽  
Marina Guillot Fernández ◽  
Ricardo Mir
Keyword(s):  
Doubled Haploid

Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Sweet Corn ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Line Production ◽  
Haploid Inducer ◽  
Breeding Programmes ◽  
Source Populations ◽  
Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Novel QTL Associated with Shoot Branching Identified in Doubled Haploid Rice (Oryza sativa L.) under Low Nitrogen Cultivation

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 745
Author(s):  
Youngho Kwon ◽  
Nkulu Rolly Kabange ◽  
Ji-Yun Lee ◽  
So-Myeong Lee ◽  
Jin-Kyung Cha ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Phenotypic Variation ◽  
Growth And Development ◽  
Oryza Sativa L ◽  
Tiller Number ◽  
Yield Potential ◽  
Doubled Haploid Population ◽  
Shoot Branching ◽  
Normal Nitrogen ◽  
Low Nitrogen

Shoot branching is considered as an important trait for the architecture of plants and contributes to their growth and productivity. In cereal crops, such as rice, shoot branching is controlled by many factors, including phytohormones signaling networks, operating either in synergy or antagonizing each other. In rice, shoot branching indicates the ability to produce more tillers that are essential for achieving high productivity and yield potential. In the present study, we evaluated the growth and development, and yield components of a doubled haploid population derived from a cross between 93-11 (P1, indica) and Milyang352 (P2, japonica), grown under normal nitrogen and low nitrogen cultivation open field conditions. The results of the phenotypic evaluation indicated that parental lines 93-11 (P1, a high tillering indica cultivar) and Milyang352 (P2, a low tillering japonica cultivar) showed distinctive phenotypic responses, also reflected in their derived population. In addition, the linkage mapping and quantitative trait locus (QTL) analysis detected three QTLs associated with tiller number on chromosome 2 (qTNN2-1, 130 cM, logarithm of the odds (LOD) 4.14, PVE 14.5%; and qTNL2-1, 134 cM, LOD: 6.05, PVE: 20.5%) and chromosome 4 (qTN4-1, 134 cM, LOD 3.92, PVE 14.5%), with qTNL2-1 having the highest phenotypic variation explained, and the only QTL associated with tiller number under low nitrogen cultivation conditions, using Kompetitive Allele-Specific PCR (KASP) and Fluidigm markers. The additive effect (1.81) of qTNL2-1 indicates that the allele from 93-11 (P1) contributed to the observed phenotypic variation for tiller number under low nitrogen cultivation. The breakthrough is that the majority of the candidate genes harbored by the QTLs qTNL2-1 and qTNN4-1 (here associated with the control of shoot branching under low and normal nitrogen cultivation, respectively), were also proposed to be involved in plant stress signaling or response mechanisms, with regard to their annotations and previous reports. Therefore, put together, these results would suggest that a possible crosstalk exists between the control of plant growth and development and the stress response in rice.

Enhanced Tolerance to Oxidative Stress with Elevated Antioxidant Capacity in Doubled Haploid Maize Derived from Microspores Exposed to Paraquat

Crop Science ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 628-636 ◽  
Author(s):  
Éva Darkó ◽  
Helga Ambrus ◽  
József Fodor ◽  
Zoltán Király ◽  
Beáta Barnabás
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Doubled Haploid

Comparative Molecular Analysis of Winter Wheat Cultivars and Their Doubled Haploid Derivatives

2001 ◽  
Vol 29 (1-2) ◽  
pp. 41-48 ◽  
Author(s):  
O. Törjék ◽  
E. Kiss ◽  
K. Mázik-Tőkei ◽  
G. Hutvágner ◽  
D. Silhavy ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Molecular Analysis ◽  
Doubled Haploid ◽  
Wheat Cultivars ◽  
Winter Wheat Cultivars

scholarly journals A New Approach to Select Doubled Haploid Rice Lines under Salinity Stress Using Indirect Selection Index

Rice Science ◽  
2021 ◽  
Vol 28 (4) ◽  
pp. 368-378
Author(s):  
Muhammad Fuad Anshori ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Sintho Wahyuning Ardie ◽  
Willy Bayuardi Suwarno
Keyword(s):  
Salinity Stress ◽  
Doubled Haploid ◽  
Selection Index ◽  
Indirect Selection ◽  
New Approach
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