scholarly journals A New Approach to Select Doubled Haploid Rice Lines under Salinity Stress Using Indirect Selection Index

Rice Science ◽  
2021 ◽  
Vol 28 (4) ◽  
pp. 368-378
Author(s):  
Muhammad Fuad Anshori ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Sintho Wahyuning Ardie ◽  
Willy Bayuardi Suwarno
2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Zine El Abidine Fellahi ◽  
Abderrahmane Hannachi ◽  
Hamenna Bouzerzour

Three selection methods including direct and indirect selection along with selection index based on the phenotypic values of eleven traits of agronomic interest were assessed for their application in F4 bread wheat progenies. Significant genetic variation existed among parents and crosses for the traits measured. The following were the most efficient indices for simultaneous selection of superior lines for yield and its components: base index of Williams, followed by the sum of ranks index of Smith and Hazel. The selection-based index provided the highest grain yield gains as compared to the other selection criteria, except for flag leaf area, indicating that the direct and indirect monotrait selection were not appropriate in the situation analyzed in this work. PCA identified Ain Abid × Mahon-Demias, Ain Abid × Rmada, and Ain Abid × El-Wifak as the most promising populations. At 5% selection intensity, the top 30 lines selected were distinguished, in comparison with the standard check Hidhab, by significant improvements in yield and yield components.


2021 ◽  
Vol 22 (7) ◽  
Author(s):  
Miftahur Rizqi Akbar ◽  
Bambang Sapta Purwoko ◽  
ISWARI SARASWATI DEWI ◽  
WILLY BAYUARDI SUWARNO ◽  
SUGIYANTA SUGIYANTA ◽  
...  

Abstract. Akbar MR, Purwoko BS, Dewi IS, Suwarno WB, Sugiyanta, Anshori MF. 2021. Agronomic and yield selection of doubled haploid lines of rainfed lowland rice in advanced yield trials. Biodiversitas 22: 3006-3012. Selection process is important step to obtain high yielding variety. This study aimed to obtain information on agronomic performance of doubled haploid lines of rainfed rice in advanced yield trials and select the best lines for multi-location yield trials. Two experiments were conducted in Bogor and Subang using a randomized complete block design for each location and consisted of 35 doubled haploid lines and two check varieties namely Ciherang and Inpari 18 as a treatment. The result revealed that interaction between genotype and environment (G x E) significantly affected all agronomic characters, except on the panicle length. Characters with high heritability value were all agronomic characters except number of productive tillers and productivity. Genotypic correlation analysis showed that number of productive tillers and weight of 1000 grains had significant and positive correlation with productivity. The selection used weighted selection index based on multivariate analysis. Fourteen lines were selected for multi-location yield trials. These lines had characteristics as follows: number of productive tillers (14.0-17.7 tillers), weight of 1000 grains (24.7-32.5 g), and productivity (5.0-6.2 ton ha-1). The selected lines can be evaluated further to obtain superior lines in multi-location trials.


2019 ◽  
Vol 47 (2) ◽  
pp. 111-118
Author(s):  
Miftahur Rizqi Akbar ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Willy Bayuardi Suwarno ◽  
Dan Sugiyanta

Rainfed rice breeding for high yield is an alternative to increase national rice production. The breeding can beaccelerated using anther culture technique. The selection of high productivity lines may be more effective if it also involvesthe traits contributing to or affecting the yield traits. This study aimed at determining suitable characters as selection criteriaand obtain a selection index model for high yielding doubled haploid rainfed rice lines. The experiment was conductedin a greenhouse of ICABOGRAD, Bogor and Sawah Baru Experimental Station, Bogor Agricultural University using arandomized complete block design (RCBD) with three replications. Thirty doubled haploid lines derived from anther cultureand two check varieties namely Ciherang and Inpari 18 were used. The results showed that plant height (TT), number of filledgrain per panicle (GI), and productivity (PRD) could be used as suitable selection characters. The determination of selectionindex obtained a selection model which was Selection index = 0.48 PRD + 0.31 JGI - 0.31 TT. This model can be used toselect high yielding doubled haploid rainfed rice lines.


PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179715 ◽  
Author(s):  
Tefera Tolera Angessa ◽  
Xiao-Qi Zhang ◽  
Gaofeng Zhou ◽  
Sue Broughton ◽  
Wenying Zhang ◽  
...  

2019 ◽  
Vol 11 (24) ◽  
pp. 2952 ◽  
Author(s):  
Sijesh Natarajan ◽  
Jayampathi Basnayake ◽  
Xianming Wei ◽  
Prakash Lakshmanan

One of the major limitations for sugarcane genetic improvement is the low heritability of yield in the early stages of breeding, mainly due to confounding inter-plot competition effects. In this study, we investigate an indirect selection index (Si), developed based on traits correlated to yield (indirect traits) that were measured using an unmanned aerial vehicle (UAV), to improve clonal assessment in early stages of sugarcane breeding. A single-row early-stage clonal assessment trial, involving 2134 progenies derived from 245 crosses, and a multi-row experiment representative of pure-stand conditions, with an unrelated population of 40 genotypes, were used in this study. Both experiments were screened at several stages using visual, multispectral, and thermal sensors mounted on a UAV for indirect traits, including canopy cover, canopy height, canopy temperature, and normalised difference vegetation index (NDVI). To construct the indirect selection index, phenotypic and genotypic variance-covariances were estimated in the single-row and multi-row experiment, respectively. Clonal selection from the indirect selection index was compared to single-row yield-based selection. Ground observations of stalk number and plant height at six months after planting made from a subset of 75 clones within the single-row experiment were highly correlated to canopy cover (rg = 0.72) and canopy height (rg = 0.69), respectively. The indirect traits had high heritability and strong genetic correlation with cane yield in both the single-row and multi-row experiments. Only 45% of the clones were common between the indirect selection index and single-row yield based selection, and the expected efficiency of correlated response to selection for pure-stand yield based on indirect traits (44%–73%) was higher than that based on single-row yield (45%). These results highlight the potential of high-throughput phenotyping of indirect traits combined in an indirect selection index for improving early-stage clonal selections in sugarcane breeding.


Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 56
Author(s):  
Ibrahim Al-Ashkar ◽  
Walid Ben Romdhane ◽  
Rania A. El-Said ◽  
Abdelhalim Ghazy ◽  
Kotb Attia ◽  
...  

Salinity majorly hinders horizontal and vertical expansion in worldwide wheat production. Productivity can be enhanced using salt-tolerant wheat genotypes. However, the assessment of salt tolerance potential in bread wheat doubled haploid lines (DHL) through agro-physiological traits and stress-related gene expression analysis could potentially minimize the cost of breeding programs and be a powerful way for the selection of the most salt-tolerant genotype. We used an extensive set of agro-physiologic parameters and salt-stress-related gene expressions. Multivariate analysis was used to detect phenotypic and genetic variations of wheat genotypes more closely under salinity stress, and we analyzed how these strategies effectively balance each other. Four doubled haploid lines (DHLs) and the check cultivar (Sakha93) were evaluated in two salinity levels (without and 150 mM NaCl) until harvest. The five genotypes showed reduced growth under 150 mM NaCl; however, the check cultivar (Sakha93) died at the beginning of the flowering stage. Salt stress induced reduction traits, except the canopy temperature and initial electrical conductivity, which was found in each of the five genotypes, with the greatest decline occurring in the check cultivar (Sakha-93) and the least decline in DHL2. The genotypes DHL21 and DHL5 exhibited increased expression rate of salt-stress-related genes (TaNHX1, TaHKT1, and TaCAT1) compared with DHL2 and Sakha93 under salt stress conditions. Principle component analysis detection of the first two components explains 70.78% of the overall variation of all traits (28 out of 32 traits). A multiple linear regression model and path coefficient analysis showed a coefficient of determination (R2) of 0.93. The models identified two interpretive variables, number of spikelets, and/or number of kernels, which can be unbiased traits for assessing wheat DHLs under salinity stress conditions, given their contribution and direct impact on the grain yield.


1977 ◽  
Vol 28 (2) ◽  
pp. 211 ◽  
Author(s):  
R Shorter ◽  
DE Byth ◽  
VE Mungomery

Three crosses of soybean involving United States and other germplasm were examined in the F3 and F4 generations at two locations, one near the coast, the other on the Darling Downs. Line and line x location interaction variances within crosses for most traits were almost all significant, except for protein, oil, and protein plus oil yield where line variances were non-significant when data were combined over locations. Line variances were considerably smaller for all traits when estimated across, rather than within, locations. For protein, oil, and protein plus oil percentage, the line x location interaction variance component generally was smaller than the line variance component in two crosses, but larger in a third even though the latter cross had the largest line variance for these traits. The line x location interactions may have been related in some cases to lower protein and oil percentages of late-maturing lines in that location where cold conditions prevailed late in the season. Heritability percentages computed from F4 variance components ranged from 49 to 89% and were largest for the chemical pexentage traits within locations. Standard unit heritability percentages estimated from F3–F4 generations were lower than those computed via the variance component method, and approached zero for oil percentage, protein plus oil percentage, and the chemical yield traits in some crosses if the generations were grown in different locations. Genotypic correlations between protein percentage and seed yield ranged from –0.50 to 0.16 across crosses and were opposite in sign to those between oil percentage and seed yield. Genotypic and phenotypic correlations between protein and oil percentage were highly negative in two crosses (–0.62 to –0.96) but much less negative in the third cross (–0.24 to –0.35). Predicted genetic advance (percentage of population mean) ranged from 5.9 to 26.1% for the chemical yield traits and from 1.5 to 6.3% for protein or oil percentage. Indirect selection for chemical yield through seed yield was 90–109% as efficient as direct selection, and a selection index that combined seed yield and either protein, oil, or protein plus oil percentage generally was no more efficient than direct selection for the corresponding chemical yield trait. Indirect selection for chemical yield through chemical percentage was much less efficient than direct selection for chemical yield.


2019 ◽  
Vol 47 (1) ◽  
pp. 1-8
Author(s):  
Nita Kartina ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Desta Wirnas ◽  
Dan Anggi Nindita

Development of doubled haploid lines of upland rice through anther culture is one way to obtain superior upland rice varieties tolerant to drought. The objectives of this research were to determine the response of doubled haploid lines to drought stress at seedling stage and to select tolerant doubled haploid lines. The research was conducted at a screenhouse of Muara Research Station, Indonesia Center of Rice Research (ICRR) from November to December 2017. Fourteen doubled haploid lines and 4 check varieties, namely Inpago 10 and Limboto, Salumpikit (drought tolerant check) and IR 20 (drought sensitive check) were used in this study. The experiment was arranged in a randomized complete block design with three replications. The observations were conducted on leaf rolling, leaf drought, and recovery ability. Based on Friedman nonparametric analysis, HR-5-7-1-1 and HR-7-15-2-1 had moderate response with estimated median values for leaf rolling of 3.44 and 3.00, respectively. Three doubled haploid lines namely HR-2-27-2-7, HR-2-34-1-3 and HR-7-15-2-1 had moderate response to drought with estimated median value of leaf drought of 3.56. Nine lines of doubled haploid had tolerant response with estimated median value of recovery ability of 1.00-1.56. Based on weighted selection index, nine doubled haploid lines with positive and high selection index were identified; however, only two lines (HR-7-15-2-1 and B3-2) had selection index higher than Inpago 10 and Limboto.Keywords: drought tolerance, leaf rolling, recovery, weighted selection index


1997 ◽  
Vol 48 (2) ◽  
pp. 207 ◽  
Author(s):  
Bakshi Ram ◽  
B. S. Chaudhary ◽  
S. Singh

Effectiveness of indirect selection criteria for brix yield based on 4 primary characters (number of millable canes per stool, NMC), cane diameter, cane height, and hand refractometer brix (HR Brix) was studied. A general index involving these traits was studied in 3 populations of ratoon seedlings. Selection based on number of millable canes was best, followed by selection based on selection index and cane height in ratoon crop. The differences in mean performance in ratoon crop disappeared at the evaluation stage in the clonal nursery under normal and restricted irrigated conditions. In general, all selection criteria, except selection based on HR Brix, gave similar responses for brix yield. Low repeatability of traits, insignificant regression coecients, and low proportion of clones outyielding the best standard variety CoH 35 at selection and evaluation stages indicated the ineffectiveness of selection at the single-stool ratoon seedlings stage. The reasons for ineffectiveness of selection based on the selection index are discussed.


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