Screening for Anticancer Activity: Trypan Blue Exclusion Assay

2022 ◽  
pp. 443-448
Author(s):  
InamulHasan Madar ◽  
Ghazala Sultan ◽  
Ramachandran Chelliah ◽  
Deog-Hwan Oh
Keyword(s):  
Anticancer Activity ◽  
Trypan Blue ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Assay

Polydopamine and dopamine interfere with tetrazolium-based cytotoxicity assays and produce exaggerated cytocompatibility inferences

2021 ◽  
Author(s):  
Anand Kumar Awasthi ◽  
Sakshi Gupta ◽  
Kavthekar Rupesh Namdev ◽  
Aditi Banerjee ◽  
Aasheesh Srivastava
Keyword(s):  
Cell Viability ◽  
Trypan Blue ◽  
Reliable Estimate ◽  
Trypan Blue Exclusion ◽  
Cytotoxicity Assays ◽  
Trypan Blue Exclusion Assay

Polydopamine (PDA) and dopamine (DA) can spontaneously reduce MTT reagent to formazan, resulting in incorrect cell-viability inferences. The non-redox Trypan Blue exclusion assay provides a more reliable estimate of cell viability with PDA and DA.

scholarly journals Trypan blue exclusion assay by flow cytometry

2014 ◽  
Vol 47 (4) ◽  
pp. 307-315 ◽  
Author(s):  
B.A. Avelar-Freitas ◽  
V.G. Almeida ◽  
M.C.X. Pinto ◽  
F.A.G. Mourão ◽  
A.R. Massensini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Trypan Blue ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Assay

Clarification of the Phenotypic Characteristics and Anti-Tumor Activity of Hedyotis diffusa

2011 ◽  
Vol 39 (01) ◽  
pp. 201-213 ◽  
Author(s):  
Hong-Zin Lee ◽  
Da-Tian Bau ◽  
Chao-Lin Kuo ◽  
Ru-Yin Tsai ◽  
Yu-Chang Chen ◽  
...  
Keyword(s):  
Trypan Blue ◽  
High Performance ◽  
Significant Inhibition ◽  
Lung Cancer Cell ◽  
Phenotypic Characteristics ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Assay ◽  
Hedyotis Diffusa ◽  
Anti Tumor Activity ◽  
Hplc Data

Hedyotis diffusa Willd. (Rubiaceae) is an important folk herb used to prevent and cure hepatitis and liver cancer in Taiwan. For differentiation of H. diffusa from counterfeits, macroscopic and microscopic characters of H. diffusa, H. corymbosa and H. tenelliflora were examined in this study. According to Trypan blue exclusion assay and Western blot analysis, H. diffusa had a significant inhibition of cell growth and induction of cell apoptosis in COLO 205 (colon cancer), Hep 3B (hepatocellular carcinoma) and H460 (lung cancer) cell lines. This study also used high-performance liquid chromatography (HPLC) to determine the quality control of H. diffusa. The HPLC data showed that ursolic and oleanolic acid are the components of the H. diffusa, consisting of approximately 4.66–4.80% and 1.86–1.96%, respectively. Our study also demonstrated that ursolic acid has significant anti-tumor activity in COLO 205, Hep 3B and H460 cancer cells.

scholarly journals Effects of curcumin and luteolin on viability and cell death induction in NFS-60 cell line

2018 ◽  
Vol 2 (2) ◽  
pp. 43
Author(s):  
Anja Haveric ◽  
Sejla Goletic ◽  
Selma Durgut ◽  
Maida Hadzic ◽  
Sanin Haveric
Keyword(s):  
Cell Death ◽  
Natural Products ◽  
Cell Line ◽  
Tumor Cells ◽  
Trypan Blue ◽  
Folk Medicine ◽  
Trypan Blue Exclusion ◽  
Induce Cell Death ◽  
Promising Strategy ◽  
Trypan Blue Exclusion Assay

Inducing cell death in tumor cells has been recognized as a promising strategy in curing tumors. Parallely, natural products, especially those with long-known usage in folk medicine, are gaining demanding and extensive clinical interests. Aiming to contribute to overall knowledge of curcumin and luteolin antitumour potentials, we analyzed their effects on cell death induction in NFS-60 cell line, using Trypan blue exclusion assay and TransDetect® Anenexin V-EGFP/PI assay. Results show that both tested agents induce cell death, especially in higher applied concentrations, but further investigations are needed to elucidate the mechanisms behind it.

scholarly journals PHYTOCHEMICAL STUDY AND THE ANTIPROLIFERATIVE ACTIVITY OF INULA VULGARIS SPECIES GROWN IN LEBANON

2017 ◽  
Vol 9 (8) ◽  
pp. 75
Author(s):  
Assi M. ◽  
Usta J. ◽  
Mounimne Y. ◽  
Aboul Ela M. ◽  
El Lakany A.
Keyword(s):  
Ethyl Acetate ◽  
Antiproliferative Activity ◽  
Trypan Blue ◽  
Heart Diseases ◽  
Trypan Blue Exclusion ◽  
Potent Effect ◽  
Phytochemical Study ◽  
Chemical Structures ◽  
13C Nuclear Magnetic Resonance ◽  
Mtt Assays

Objective: Cancer represents the second leading cause of death after stroke and heart diseases. Plant extracts have long been used in traditional medicine for the prevention and treatment of many illnesses, including some types of cancer. The aim of this study was to evaluate the antiproliferative effects of ethyl acetate fractions of two Lebanese herbs: Inula viscosa (I. vis) and Inula vulgaris (I. vul).Methods: Plants were extracted with ethanol followed by ethyl acetate, then dried and tested on three cell lines including CaCO2, HepG2, and MCF7, to check for their viability and antiproliferative activity, using trypan blue exclusion and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Proton (1H) and carbon (13C) nuclear magnetic resonance spectrometry (NMR) were used to identify the compounds that have been isolated from both Inula species.Results: The current findings were consistent for both trypan blue and MTT assays. The results showed that the most potent effect for I. vul was HepG2 (IC50 20 µg/ml, 27 µg/ml), and for I. vis on MCF7 (9 µg/ml, 15 µg/ml) and CaCO2 (12 µg/ml, 22 µg/ml) in the two mentioned assays respectively. However, insignificant differences were observed among the studied plants for each of the evaluated cells indicating comparable potencies. Quercetin, quercetin glycoside, and epicatechin derivatives were isolated by fractionation on column chromatography and identified using NMR spectroscopy.Conclusion: The antiproliferative activities of the two plants could be related to their content that is significant for high levels of secondary metabolites. The identification of those compounds is necessary to establish a relationship between their chemical structures and their activities.

scholarly journals Microprocessor-controlled vs. "dump-freezing" platelet and lymphocyte cryopreservation: A quantitative and qualitative comparative study

2006 ◽  
Vol 63 (3) ◽  
pp. 261-268 ◽  
Author(s):  
Bela Balint ◽  
Dusan Vucetic ◽  
Biljana Draskovic ◽  
Danilo Vojvodic ◽  
Goran Brajuskovic ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Trypan Blue ◽  
Proliferative Response ◽  
Antigen Expression ◽  
Intergroup Difference ◽  
Cell Recovery ◽  
Trypan Blue Exclusion ◽  
Rate System ◽  
Platelet Recovery ◽  
Cell Percentage

Background/Aim. Thermodynamical and cryobiological parameters responsible for cell damages during cryopreservation (cryoinjuries) have not yet been completely explained. Thus, freezing procedures should be revised, exactly optimized to obtain an enhanced structural and functional recovery of frozen- thawed cells. The aim of this study was to compare microprocessor- controlled (controlled-rate) with the compensation of the released fusion heat and ?dump-freezing? (uncontrolled- rate) of the platelet and lymphocyte cryopreservation efficacy. Methods. Platelet quantitative recovery (post-thaw vs. unfrozen cell count), viability (using hypotonic shock response - HSR), morphological score (PMS), ultrastructural (electron microscopy) properties and expression of different surface antigens were investigated. In lymphocyte setting, cell recovery and viability (using trypan blue exclusion test) as well as functionality (by plant mitogens) were determined. Controlled- rate freezing and uncontrolled-rate cryopreservation were combined with 6% (platelets) and 10% (lymphocytes) dimethyl sulfoxide (DMSO). Results. Platelet recovery and functionality were superior in the controlled-rate system. The majority of surface antigen expression was reduced in both freezing groups vs. unfrozen cells, but GP140/CD62p was significantly higher in controlled-rate vs. uncontrolled-rate setting. Controlled- rate freezing resulted with better lymphocyte recovery and viability (trypan blue-negative cell percentage). In mitogen-induced lymphocyte proliferative response no significant intergroup difference (controlled-rate vs. uncontrolled-rate) were found. Conclusion. The data obtained in this study showned the dependence of cell response on the cryopreservation type. Controlled-rate freezing provided a superior platelet quantitative and functional recovery. Lymphocyte recovery and viability were better in the controlled-rate group, although only a minor intergroup difference for cell proliferative response was obtained.

Effects of medium and hypothermic temperatures on preservation of isolated porcine testis cells

2010 ◽  
Vol 22 (3) ◽  
pp. 523 ◽  
Author(s):  
Yanfei Yang ◽  
Ali Honaramooz
Keyword(s):  
Trypan Blue ◽  
Cell Isolation ◽  
Live Cell ◽  
Live Cells ◽  
Cell Recovery ◽  
Trypan Blue Exclusion ◽  
Culture Studies ◽  
Porcine Testis ◽  
Peritubular Cells

The effects of medium and hypothermic temperatures on testis cells were investigated to develop a strategy for their short-term preservation. Testes from 1-week-old piglets were enzymatically dissociated for cell isolation. In Experiment 1, testis cells were stored at either room (RT) or refrigeration (RG) temperature for 6 days in one of 13 different media. Live cell recovery was assayed daily using trypan blue exclusion. In Experiment 2, three media at RG were selected for immunocytochemical and in vitro culture studies. Live cell recovery was also assayed daily for 6 days using both trypan blue exclusion and a fluorochrome assay kit. For all media tested, significantly or numerically more live cells were maintained at RG than RT. On preservation Day 3 at RG (cell isolation day as Day 0), 20% FBS-Leibovitz resulted in the highest live cell recovery (89.5 ± 1.7%) and DPBS in the lowest (60.3 ± 1.9%). On Day 6 at RG, 20% FBS- Leibovitz also resulted in the best preservation efficiency with 80.9 ± 1.8% of Day 0 live cells recovered. There was no difference in live cell recovery detected by the two viability assays. After preservation, the proportion of gonocytes did not change, whereas that of Sertoli and peritubular cells increased and decreased, respectively. After 6 days of hypothermic preservation, testis cells showed similar culture potential to fresh cells. These results show that testis cells can be preserved for 6 days under hypothermic conditions with a live cell recovery of more than 80% and after-storage viability of 88%.

scholarly journals Effect of lead on proliferation, oxidative stress and genotoxic damage of 3T3-L1 fibroblasts

2020 ◽  
Vol 9 (3) ◽  
pp. 158-163
Author(s):  
Claudia Noemi Martini ◽  
Fernando Nicolás Sosa ◽  
Julio Fuchs ◽  
María del Carmen Vila
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Cell Line ◽  
Public Health Problem ◽  
Cell Counting ◽  
Trypan Blue Exclusion ◽  
Genotoxic Damage ◽  
Trypan Blue Exclusion Assay ◽  
A Cell ◽  
Increase Lipid Peroxidation

Abstract Lead (Pb) is an environmental and industrial contaminant that still represents a public health problem. In this paper, we investigated the effect of Pb on proliferation, lipid peroxidation and the number of micronucleated cells in exponentially growing 3T3-L1 fibroblasts, a cell line previously used to evaluate different environmental contaminants. We found that Pb (10 μM or higher) was able to inhibit proliferation of exponentially growing cells after 24-h treatment, which was evaluated by the MTT assay and cell counting in Neubauer chamber, but cell survival was not affected according to the trypan blue exclusion assay. On the other hand, Pb was able to increase lipid peroxidation and the number of micronucleated cells, which are indicative of oxidative stress and genotoxic damage respectively. We also found that removal of Pb after 24-h treatment allowed cells to recover proliferation. Our results indicate that Pb was able to induce oxidative stress and genotoxicity in this cell line under standardized conditions, which supports the involvement of Pb in similar effects observed in human exposed to this heavy metal. In addition, Pb inhibits proliferation of exponentially growing fibroblasts but cells resume proliferation after removal of this metal, which suggests that it is important to move away Pb-exposed individuals from the source of contamination.

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