In-Situ Dissections of Brain with Emphasis on the Blood Supply at the Base of the Brain

Author(s):  
Margaret M. Waddington
Keyword(s):  
1992 ◽  
Vol 57 (1) ◽  
pp. 194-203 ◽  
Author(s):  
Karel Šindelář ◽  
Vojtěch Kmoníček ◽  
Marta Hrubantová ◽  
Zdeněk Polívka

(Arylthio)benzoic acids IIa - IIe and VIb - VId were transformed via the acid chlorides to the N,N-dimethylamides which were reduced either with diborane "in situ" or with lithium aluminium hydride to N,N-dimethyl-(arylthio)benzylamines Ia - Ie and Vb - Vd. Leuckart reaction of the aldehydes IX and X with dimethylformamide and formic acid afforded directly the amines Va and Ve. Demethylation of the methoxy compounds Ia and Ve with hydrobromic acid resulted in the phenolic amines If and Vf. The most interesting N,N-dimethyl-4-(phenylthio)benzylamine (Va) hydrochloride showed affinity to cholinergic and 5-HT2 serotonin receptors in the rat brain and some properties considered indicative of antidepressant activity (inhibition of serotonin re-uptake in the brain and potentiation of yohimbine toxicity in mice).


Development ◽  
1993 ◽  
Vol 119 (1) ◽  
pp. 247-261 ◽  
Author(s):  
B.A. Parr ◽  
M.J. Shea ◽  
G. Vassileva ◽  
A.P. McMahon

Mutation and expression studies have implicated the Wnt gene family in early developmental decision making in vertebrates and flies. In a detailed comparative analysis, we have used in situ hybridization of 8.0- to 9.5-day mouse embryos to characterize expression of all ten published Wnt genes in the central nervous system (CNS) and limb buds. Seven of the family members show restricted expression patterns in the brain. At least three genes (Wnt-3, Wnt-3a, and Wnt-7b) exhibit sharp boundaries of expression in the forebrain that may predict subdivisions of the region later in development. In the spinal cord, Wnt-1, Wnt-3, and Wnt-3a are expressed dorsally, Wnt-5a, Wnt-7a, and Wnt-7b more ventrally, and Wnt-4 both dorsally and in the floor plate. In the forelimb primordia, Wnt-3, Wnt-4, Wnt-6 and Wnt-7b are expressed fairly uniformly throughout the limb ectoderm. Wnt-5a RNA is distributed in a proximal to distal gradient through the limb mesenchyme and ectoderm. Along the limb's dorsal-ventral axis, Wnt-5a is expressed in the ventral ectoderm and Wnt-7a in the dorsal ectoderm. We discuss the significance of these patterns of restricted and partially overlapping domains of expression with respect to the putative function of Wnt signalling in early CNS and limb development.


2017 ◽  
Vol 24 (3) ◽  
pp. 244
Author(s):  
B. Domingo-Arrué ◽  
R. Gil-Benso ◽  
J. Megías ◽  
L. Navarro ◽  
T. San-Miguel ◽  
...  

We report a case of a recurrent clear cell meningioma (ccm) in the frontal lobe of the brain of a 67-year-old man. The patient developed three recurrences: at 3, 10, and 12 years after his initial surgery. Histopathology observations revealed a grade 2 ccm with positivity for vimentin and epithelial membrane antigen. Expression of E-cadherin was positive only in the primary tumour and in the first available recurrence. Fluorescence in situ hybridization analyses demonstrated 1p and 14q deletions within the last recurrence. Multiplex ligation-dependent probe amplification studies revealed a heterozygous partial NF2 gene deletion, which progressed to total loss in the last recurrence. The last recurrence showed homozygous deletions in CDKN2A and CDKN2B. The RASSF1 gene was hypermethylated during tumour evolution.In this report, we show the genetic alterations of a primary ccm and its recurrences to elucidate their relationships with the changes involved in the progression of this rare neoplasm.


2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Kazuyuki Mitsuoka ◽  
Yoko Miwa ◽  
Takeshi Kikutani ◽  
Iwao Sato

The neuropeptide calcitonin gene-related peptide (CGRP) mediates inflammation and head pain by influencing the functional vascular blood supply. CGRP is a well-characterized mediator of receptor-regulated neurotransmitter release. However, knowledge regarding the role of CGRP during the development of the superior cervical ganglion (SCG) is limited. In the present study, we observed the localization of CGRP and vascular endothelial growth factor (VEGF-A) mRNAs during prenatal development at embryonic day 14.5 (E14.5), E17.5 and postnatal day 1 (P1) using in situ hybridization. The antisense probe for CGRP was detected by in situ hybridization at E14.5, E17.5, and P1, and the highest levels were detected at E17.5. In contrast, the antisense probe for VEGF-A was detected by in situ hybridization in gradually increasing intensity from E14.5 to P1. The differences in the expression of these two markers revealed specific characteristics related to CGRP concentration and release compared to those of VEGF-A during development. The correlation between CGRP and VEGF-A may influence functional stress and the vascular blood supply during prenatal and postnatal development.


1968 ◽  
Vol 214 (6) ◽  
pp. 1225-1249 ◽  
Author(s):  
M Viamonte ◽  
PJ Morgane ◽  
RE Galliano ◽  
EL Nagel ◽  
WL McFarland
Keyword(s):  

Rhetorik ◽  
2018 ◽  
Vol 37 (1) ◽  
pp. 68-93
Author(s):  
Markus H. Woerner ◽  
Ricca Edmondson

Abstract Using an understanding of rhetoric as a method of communicative reasoning capable of providing grounds for conviction in those to whom it is addressed, this article argues that the formation of medical diagnoses shares a structure with Aristotle’s account of the rhetorical syllogism (the enthymeme). Here the argument itself (logos), together with characterological elements (ethos) and emotions (pathos), are welded together so that each affects the operation of the others. In the initial three sections of the paper, we contend, first, that diagnoses, as verdictive performatives, differ from scientific claims in being irreducibly personal and context-dependent; secondly, that they fit the structure of voluntary action as analysed by Aristotle and Aquinas; thirdly, that as practical syllogisms they differ from theoretical syllogisms, for example in taking effect in action, being ›addressed‹, and being intrinsically embedded in wider contexts of medical communication and practices. In the remaining sections we apply this account to textual evidence about diagnosis, drawing on work by the brain surgeon Henry Marsh. A rhetorical analysis of his observations on the formation of diagnostic opinions in situilluminates how moral, social and emotional features are fused with the cognitive aspects of medical judgement, making or marring how diagnoses and treatment are enacted. In other words, a philosophical- rhetorical account of diagnosis can help us to appreciate how medical diagnosis takes effect. We briefly conclude with some implications of our work for how diagnostic processes could in practice be better supported.


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