scholarly journals Identification of cDNAs by Direct Hybridization Using Cosmid Probes

1994 ◽  
pp. 23-28
Author(s):  
Gregory G. Lennon ◽  
Kimberly Lieuallen
Keyword(s):  
Direct Hybridization

An Investigation of Half-Metallic Ferromagnets Behavior in Hg2CuTi-Type Heusler Alloy Ti2FeAl by Using GGA

2012 ◽  
Vol 535-537 ◽  
pp. 1291-1294 ◽  
Author(s):  
Xiu De Yang ◽  
Bo Wu ◽  
Song Zhang
Keyword(s):  
Density Functional Theory ◽  
Heusler Alloy ◽  
Density Functional ◽  
Generalized Gradient ◽  
Functional Theory ◽  
Half Metallic ◽  
Generalized Gradient Approximation ◽  
Indirect Exchange ◽  
The Density Functional Theory ◽  
Direct Hybridization

By using generalized gradient approximation (GGA) scheme within the density functional theory (DFT), the electronic and magnetic properties of Hg2CuTi-type Heusler alloy Ti2FeAl were investigated. The results reveal that a 100% spin polarization appears at Fermi level (εF) in Ti2FeAl, and is maintained during lattice range of 5.1Å~6.2Å. Ti2FeAl is one of stable Half-Metallic Ferromagnets (HMF) with a spin-minority gap of 0.5 eV at εF and total magnetic moment of 1μB per unit cell. Our studies also indicate that the competition between RKKY-type indirect exchange and direct hybridization of d-electronic atoms plays a dominating role in determining the magnetism.

Development and characterization of Triticum turgidum – Aegilops comosa and T. turgidum – Ae. markgrafii amphidiploids

Genome ◽  
2020 ◽  
Vol 63 (5) ◽  
pp. 263-273
Author(s):  
Yuanyuan Zuo ◽  
Qin Xiang ◽  
Shoufen Dai ◽  
Zhongping Song ◽  
Tingyu Bao ◽  
...  
Keyword(s):  
Genetic Improvement ◽  
Triticum Turgidum ◽  
Tetraploid Wheat ◽  
Diploid Species ◽  
Unreduced Gametes ◽  
Stripe Rust Resistance ◽  
Aegilops Comosa ◽  
Direct Hybridization

Aegilops comosa and Ae. markgrafii are diploid progenitors of polyploidy species of Aegilops sharing M and C genomes, respectively. Transferring valuable genes/traits from Aegilops into wheat is an alternative strategy for wheat genetic improvement. The amphidiploids between diploid species of Aegilops and tetraploid wheat can act as bridges to overcome obstacles from direct hybridization and can be developed by the union of unreduced gametes. In this study, we developed seven Triticum turgidum – Ae. comosa and two T. turgidum – Ae. markgrafii amphidiploids. The unreduced gametes mechanisms, including first-division restitution (FDR) and single-division meiosis (SDM), were observed in triploid F1 hybrids of T. turgidum – Ae. comosa (STM) and T. turgidum – Ae. markgrafii (STC). Only FDR was observed in STC hybrids, whereas FDR or both FDR and SDM were detected in the STM hybrids. All seven pairs of M chromosomes of Ae. comosa and C chromosomes of Ae. markgrafii were distinguished by fluorescent in situ hybridization (FISH) probes pSc119.2 and pTa71 combinations with pTa-535 and (CTT)12/(ACT)7, respectively. Meanwhile, the chromosomes of tetraploid wheat and diploid Aegilops parents were distinguished by the same FISH probes. The amphidiploids possessed specific valuable traits such as multiple tillers, large seed size related traits, and stripe rust resistance that could be utilized in the genetic improvement of wheat.

Synergistic effects of progesterone and oestradiol on rat LH subunit mRNA

1990 ◽  
Vol 4 (2) ◽  
pp. 119-125 ◽  
Author(s):  
M. Corbani ◽  
R. Counis ◽  
E. Wolinska-Witort ◽  
G. d'Angelo-Bernard ◽  
M. Moumni ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Synergistic Effects ◽  
Progesterone Receptors ◽  
Ovariectomized Rats ◽  
Mrna Levels ◽  
Subunit Mrna ◽  
Maximum Inhibition ◽  
Lh Release ◽  
Direct Hybridization

ABSTRACT The effects of oestradiol and progesterone on LH-subunit mRNA levels were investigated in ovariectomized rats. Four weeks after ovariectomy, rats were implanted with silicone elastomer capsules containing oestradiol and/or injected daily with progesterone in oil (5 mg/rat) for 8 days. The levels of pituitary mRNA encoding α and LH-β were determined using direct hybridization with specific [32P]cDNA probes. After oestradiol implantation in ovariectomized rats, both α and LH-β mRNA decreased with time, with maximum inhibition after 6–8 days of treatment. Progesterone injected alone did not show any effect on α and LH-β mRNA. Cytosolic progesterone receptors, determined using [3H]methyl-17α-progesterone as ligand, were undectable in control ovariectomized rats. In contrast, 2 days after oestradiol implantation, the number of receptors increased to 287·5 ± 35·4 (s.e.m.) fmol/pituitary and reached a plateau of 400 ± 21·8 fmol/pituitary after 4 days. The effects of progesterone were therefore examined by first implanting ovariectomized rats with oestradiol to induce progesterone receptors and then injecting progesterone daily for a further period of 6 days. As a result of this treatment, progesterone induced a decrease in the pituitary gland contents of both α and LH-β mRNAs, and LH release was significantly greater than that observed in the group receiving oestradiol alone. Moreover, the mRNA levels in the animals treated with oestradiol plus progesterone were lower after 8 days of treatment than those observed in ovariectomized rats treated with a tenfold higher dose of oestradiol alone. These data demonstrate that progesterone, together with oestradiol, is capable of negatively regulating the mRNAs encoding subunits in vivo, provided that progesterone receptors are present in the pituitary gland.

Tunable Magnetism and Half-Metallic Stability in Si-Doped Hg2CuTi-Type Ti2CoGa Alloy

2012 ◽  
Vol 229-231 ◽  
pp. 130-133 ◽  
Author(s):  
Bo Wu ◽  
Yu Feng ◽  
Hong Kuan Yuan ◽  
Hong Chen
Keyword(s):  
Density Functional ◽  
Magnetic Moments ◽  
Half Metallicity ◽  
Functional Theory ◽  
Half Metallic ◽  
Lattice Constants ◽  
Indirect Exchange ◽  
The Density Functional Theory ◽  
Si Doped ◽  
Direct Hybridization

Using the ab-initio calculations within the density functional theory (DFT), we have investigated the electronic structure, magnetism and half-metallic stability of Si-doped Heusler compound Ti2CoGa with Hg2CuTi-type structure. The results revel that the lattice constants and total magnetic moments in per unit obey the Vegard’s rule and the Slater-Pauling rule well, respectively. The most stable half-metallicity occurs at doping concentration x=0.75 because the Fermi level is located at the middle of the spin-minority gap. Our studies also indicate that the competition between RKKY-type indirect exchange and direct hybridization of d-electronic atoms plays a dominating role in determining the magnetism.

scholarly journals Heat Generation by Electrical Current in a Quantum Dot Hybridized to Majorana Nanowires

2021 ◽  
Vol 9 ◽  
Author(s):  
Zhu-Hua Wang
Keyword(s):  
Quantum Dot ◽  
Bias Voltage ◽  
Heat Generation ◽  
Bound States ◽  
Waste Heat ◽  
Electrical Current ◽  
Function Technique ◽  
Heat Current ◽  
Low Dimensional ◽  
Direct Hybridization

Heat current generated by electronic transport through a quantum dot (QD) coupled to both a phonon bath and a Majorana nanowire hosting Majorana bound states (MBSs) is theoretically studied in the framework of non-equilibrium Green’s function technique. The calculated numerical results show that electrical current can be either enhanced or suppressed by the combined influences of the phonon bath and the MBSs at certain bias voltage regimes. The enhancement and suppression of the current’s magnitude for a fixed bias voltage will be reversed due to the direct hybridization between the MBSs. The simultaneous coupling between both MBSs will amplify the function of the MBSs on the current, with the same unchanged and essential qualitative impacts. Heat generation by the electrical current can be fully adjusted by the dot–MBS coupling, direct hybridization between the MBSs, and positions of the dot level. By properly choosing the above parameters, heat generation can be suppressed even for increased electrical current, which is favorable in removing waste heat generated by electrical current flowing through low-dimensional circuits.

scholarly journals Direct fuel cell—supercapacitor hybrid power source for personal suburban transport

AIMS Energy ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1274-1298
Author(s):  
Caroline Bonnet ◽  
◽  
Stéphane Raël ◽  
Melika Hinaje ◽  
Sophie Guichard ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Electric Vehicles ◽  
Test Bench ◽  
Cell Voltage ◽  
Power Source ◽  
Storage Device ◽  
Power Demand ◽  
Order Of Magnitude ◽  
External Power ◽  
Direct Hybridization

<abstract> <p>In view to proposing an alternative to oversized energy sources currently installed in electric vehicles for suburban transport, a direct hybrid fuel cell (FC)-supercapacitors (SC) source has been designed and tested on a test bench. The rated 15.6 kW source—with an air-cooled 5.6 kW FC and a 165 F SC storage device—was shown perfectly suited to traction of a 520 kg vehicle along the NEDC cycle, then validating the previously developed concept of a one-ton car propelled by a 10 kW FC in the rated 30 kW hybrid source for this cycle. In comparison with a FC used alone, hybridization was shown to allow the power demand for the cell to vary in quite a narrower range, as formerly observed. Moreover, the rates of fuel cell voltage and current generated in the driving cycle, were shown to be reduced by one order of magnitude by the direct hybridization which is to contribute to the FC durability. Two operating parameters were shown to have a significant effect on the hybrid source efficiency, namely the capacity of the SC at 110 or 165 F, and the recovery of deceleration power—emulated by an external power supply—which can decrease by 25% the fuel consumption in NEDC cycle conditions, as predicted by the model.</p> </abstract>

Sign in / Sign up

Export Citation Format

Share Document