Relationship between Intraellular dCTP/Ara-CTP Ratio and Cytotoxic Effect of Ara-C

Кidney injury is a frequent and significant complication of cancer and cancer therapy. The kidneys are susceptible to injury from malignant infiltration, damage by metabolites of malignant cells, glomerular injury, nephrotoxic drugs including chemotherapeutic agents. Also bone marrow transplantation complications, infections with immune suppression (including septicemia), tumor lysis syndrome should be taken into account. Chemotherapeutic agents are a common cause of acute kidney injury but can potentially lead to chronic kidney disease development in cancer patients. This article summarizes risk factors of acute kidney injury in cancer patients. Risk factors are divided into two groups. The systemic are decrease of total circulating blood volume, infiltration of kidney tissue by tumor cells, dysproteinemia, electrolyte disturbances. The local (renal) risk factors are microcirculation disturbances, drugs biotransformation with formation of reactive oxygen intermediates, high concentration of nephrotoxic agents in proximal tubules and its sensitivity to ischemia. Drug-related risk factors include: drugs combination with cytotoxic effect high doses long term use necessity, direct cytotoxic effect of not only chemotherapeutic agents but also its metabolites, mean solubility forming intratubular precipitates. Early diagnosis, timely prevention and treatment of these complications provide significantly improve nononcologic results of treatment.

Download Full-text

Cytotoxic Effect of Pseudomonas aurogenosa Protease on Cancer Cells

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.3.7 ◽

2019 ◽

Vol 10 (03) ◽

Author(s):

Ghanyia J. Shanyoor ◽

Fatima R. Abdul ◽

Nehad A. Taher ◽

Ihsan A. Raheem

Keyword(s):

Cell Line ◽

Normal Cell ◽

Cytotoxic Effect ◽

Human Cancer ◽

Skim Milk ◽

Exchange Chromatography ◽

Protease Production ◽

Normal Cell Line ◽

Protease Enzyme ◽

Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) and the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method and it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column and the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , and one normal cell line Ref ( Rat embryonic fibroblast ) . The cancer and normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8and 0.16 mg/ml) then incubated for additional 48h at 37C0 and the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andslight toxicity ( 37.12% ) on normal cell line (Ref) in a concentration (0.8mg/ml).

Download Full-text

Anti-carcinogenic and cytotoxic effect of Carthamus oxycantha Safflower seed oil extract on Hela cell lines

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2019.11.01.047 ◽

2019 ◽

Vol 11 (1) ◽

Keyword(s):

Hela Cell ◽

Cell Lines ◽

Cytotoxic Effect ◽

Seed Oil ◽

Safflower Seed ◽

Safflower Seed Oil ◽

Hela Cell Lines ◽

Oil Extract

Download Full-text

CITOTOXICITY OF INDIUM-111-LABELED AUTOLOGOUS WBC

Токсикологический вестник ◽

10.36946/0869-7922-2019-1-18-22 ◽

2019 ◽

pp. 18-22

Author(s):

K. A. Luneva ◽

K. E. Ternovskaya ◽

O. E. Klement’eva ◽

A. S. Lunev

Keyword(s):

Cytotoxic Effect ◽

Single Photon ◽

Photon Emission ◽

Emission Computed Tomography ◽

Blood Leukocytes ◽

Cytotoxic Effects ◽

Indium 111 ◽

Single Photon Emission Computed ◽

Photon Emission Computed Tomography ◽

Subsequent Introduction

The radiopharmaceutical precursor «Oxind, 111In» is a complex compound for labeling autologous leukocytes of human blood and their subsequent introduction for non-specific visualization and localization of inflammation foci of different nature by single-photon emission computed tomography (SPECT). The cytotoxic effect of lyophilisate for preparation of radiopharmaceutical preparation «Oxind, 111In» and its radiopharmaceutical precursor on rabbit blood leukocytes has been investigated. In the course of studies of the cytotoxicity of the lyophilisate for the preparation of a radiopharmaceutical precursor with successively increasing concentrations of the main substance, 8-hydroxyquinoline, the permissible concentrations have been determined and the absolutely cytotoxic concentration was achieved.In the study of the cytotoxic effect of the precursor of radiopharmaceutical on two dosages that differ by 10 times in volume activities (MBq / ml), the absence of cytotoxic effects has been confirmed.

Download Full-text

Cytotoxic Effect of Isolated Protein-bound Polysaccharides from Hypsizigus marmoreus Extracts by Response Surface Methodology

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2008.37.12.1647 ◽

2008 ◽

Vol 37 (12) ◽

pp. 1647-1653 ◽

Cited By ~ 3

Author(s):

Eun-Bong Jung ◽

Jin-Ho Jo ◽

Seung-Mock Cho

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Cytotoxic Effect ◽

Hypsizigus Marmoreus

Download Full-text

In vitro Cytotoxic Effect of Extracts from Styela plicata

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2007.36.9.1099 ◽

2007 ◽

Vol 36 (9) ◽

pp. 1099-1105 ◽

Cited By ~ 2

Author(s):

Bo-Bae Lee ◽

Mi-Ran Cha ◽

Hae-Ryong Park ◽

Seung-Cheol Lee

Keyword(s):

Cytotoxic Effect ◽

Styela Plicata

Download Full-text

Laminin 521 Modulates the Сytotoxic Effect of 5-Fluorouracil on Colorectal Cancer HT29 Cells

Biotekhnologiya ◽

10.21519/0234-2758-2019-35-6-73-79 ◽

2019 ◽

Vol 35 (6) ◽

pp. 73-79

Author(s):

A. Turchinovich ◽

I.M. Tsypina ◽

V.G. Zgoda ◽

S.V. Nikulin ◽

D.V. Maltseva

Keyword(s):

Colorectal Cancer ◽

Cytotoxic Effect ◽

Mechanisms Of Action ◽

Ht29 Cell ◽

Β1 Integrin ◽

Russian Science ◽

Apoptotic Gene ◽

Ht29 Cells ◽

First Time ◽

Ht29 Cell Line

The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription. apoptosis, β1 integrin, colorectal cancer, laminin 521 (laminin-11), regorafenib, 5-fluorouracil, HT29, ITGB1, LAMA5, YAP, SFN, 14-3-3σ The study was funded by the Russian Science Foundation (Project 17-14-01338).

Download Full-text