scholarly journals Western Blotting of High and Low Molecular Weight Proteins Using Heat

2015 ◽  
pp. 247-255 ◽  
Author(s):  
Biji T. Kurien ◽  
R. Hal Scofield
Keyword(s):  
Molecular Weight ◽  
Western Blotting ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins

scholarly journals Optimization of western blotting for the detection of proteins of different molecular weight

BioTechniques ◽  
2020 ◽  
Vol 68 (6) ◽  
pp. 318-324
Author(s):  
Ghanshyam D Heda ◽  
Lisa Shrestha ◽  
Sagarina Thapa ◽  
Shreya Ghimire ◽  
Diptika Raut
Keyword(s):  
Molecular Weight ◽  
Western Blotting ◽  
Low Molecular Weight ◽  
Transmembrane Conductance Regulator ◽  
High Molecular Weight Protein ◽  
Transmembrane Conductance ◽  
Weight Protein ◽  
Optimized Conditions ◽  
Cystic Fibrosis Transmembrane ◽  
Low Molecular Weight Proteins

Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended to medium- and low-molecular-weight proteins (LAMP1 and Rab11a, respectively) to determine the effects of methanol concentration (0–20%) in Towbin’s transfer buffer (TTB). Methanol in TTB appears to have little to no effect on CFTR signal. However, for medium-sized (LAMP1) and small (Rab11a) proteins, a lower concentration of methanol (10%) was sufficient to produce a maximal signal. Therefore, methanol, a toxic solvent, can be removed from or reduced in TTB without compromising signal strength. Here, we show modifications that may be useful in detecting and/or improving the signal of low-abundance proteins.

scholarly journals Synthesis of unique low molecular weight proteins during late G2 and mitosis.

1985 ◽  
Vol 260 (2) ◽  
pp. 695-698
Author(s):  
J T Westwood ◽  
E B Wagenaar ◽  
R B Church
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins

Inhibition of Copper-Associated Erythrocyte Ghost Membrane Lipid Peroxidation by Hepatic Cytosolic Low Molecular Weight Proteins

1991 ◽  
Vol 19 (3) ◽  
pp. 206-213
Author(s):  
Bruce L. Homer ◽  
Kenneth R. Pierce ◽  
Charles H. Bridges ◽  
James E. Womack ◽  
Blair A. Sowa ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Molecular Weight ◽  
Membrane Lipid ◽  
Erythrocyte Ghost ◽  
Low Molecular Weight ◽  
Membrane Lipid Peroxidation ◽  
Low Molecular Weight Proteins

scholarly journals Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

Molecules ◽  
2017 ◽  
Vol 22 (10) ◽  
pp. 1712 ◽  
Author(s):  
Sergio Hernandez-Leon ◽  
Jose Sarabia-Sainz ◽  
Gabriela Montfort ◽  
Ana Guzman-Partida ◽  
Maria Robles-Burgueño ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Silica Nanoparticles ◽  
Core Shell ◽  
Low Molecular Weight ◽  
Novel Synthesis ◽  
Low Molecular Weight Proteins ◽  
Proteins And Peptides

No Abnormal Low Molecular Weight Proteins Identified in Human Malignant Hyperthermic Muscle

1986 ◽  
Vol 64 (6) ◽  
pp. 795-797 ◽  
Author(s):  
Toni Whistler ◽  
Hyam Isaacs ◽  
Margaret Badenhorst
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins
Sign in / Sign up

Export Citation Format

Share Document