Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells

2013 ◽  
pp. 123-132
Author(s):  
Mirjam Andreasson-Ochsner ◽  
Erik Reimhult
Keyword(s):  
Three Dimensional ◽  
Adhesion Proteins ◽  
Three Dimensional Presentation

Methods for making stereoslides and -prints, with freeze-etched olfactory epithelium as example

1984 ◽  
Vol 42 ◽  
pp. 704-705
Author(s):  
Bert Ph. M. Menco ◽  
Ido F. Menco ◽  
Frans L.T. Verdonk
Keyword(s):  
Electron Microscope ◽  
Olfactory Epithelium ◽  
Three Dimensional ◽  
Supporting Cell ◽  
Structural Features ◽  
Extensive Study ◽  
Sprague Dawley ◽  
Freezing Method ◽  
Respiratory Epithelia ◽  
Three Dimensional Presentation

Previously we presented an extensive study of the distributions of intramembranous particles of structures in apical surfaces of nasal olfactory and respiratory epithelia of the Sprague-Dawley rat. For the same structures these distributions were compared in samples which were i) chemically fixed and cryo-protected with glycerol before cryo-fixation, after excision, and ii)ultra-rapidly frozen by means of the slam-freezing method. Since a three-dimensional presentation markedly improves visualization of structural features micrographs were presented as stereopairs. Two exposures were made by tiling the sample stage of the electron microscope 6° in either direction with an eucentric goniometer. The negatives (Agfa Pan 25 Professional) were reversed with Kodak Technical Pan Film 2415 developed in D76 1:1. The prints were made from these reversed negatives. As an example tight-junctional features of an olfactory supporting cell in a region where this cell conjoined with two other cells are presented (Fig. 1).

scholarly journals Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

2020 ◽  
Vol 21 (5) ◽  
pp. 1749 ◽  
Author(s):  
Thomas J. Bauer ◽  
Erich Gombocz ◽  
Markus Wehland ◽  
Johann Bauer ◽  
Manfred Infanger ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Metastasis ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Binding Activity ◽  
Omics Data ◽  
Adhesion Proteins ◽  
Adhesion Behavior

The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these cells are modified. To understand the mechanisms underlying these changes, proteins involved in cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding changes during exposure to microgravity were investigated. As the sialylation of adhesion proteins is influencing cell adhesion on Earth in vitro and in vivo, we analyzed the sialylation of cell adhesion molecules detected by omics studies on cells, which change their adhesion behavior when exposed to microgravity. Using a knowledge graph created from experimental omics data and semantic searches across several reference databases, we studied the sialylation of adhesion proteins glycosylated at their extracellular domains with regards to its sensitivity to microgravity. This way, experimental omics data networked with the current knowledge about the binding of sialic acids to cell adhesion proteins, its regulation, and interactions in between those proteins provided insights into the mechanisms behind our experimental findings, suggesting that balancing the sialylation against the de-sialylation of the terminal ends of the adhesion proteins’ glycans influences their binding activity. This sheds light on the transition from two- to three-dimensional growth observed in microgravity, mirroring cell migration and cancer metastasis in vivo.

scholarly journals Phenomenal Causality and Sensory Realism

i-Perception ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 204166952092703
Author(s):  
Kristof Meding ◽  
Sebastian A. Bruijns ◽  
Bernhard Schölkopf ◽  
Philipp Berens ◽  
Felix A. Wichmann
Keyword(s):  
Visual Perception ◽  
Three Dimensional ◽  
The Other ◽  
Two Dimensional ◽  
Perception Of Causality ◽  
Phenomenal Causality ◽  
Intuitive Physics ◽  
Three Dimensional Presentation ◽  
Cue Conflict ◽  
Motion Dynamics

One of the most important tasks for humans is the attribution of causes and effects in all wakes of life. The first systematical study of visual perception of causality—often referred to as phenomenal causality—was done by Albert Michotte using his now well-known launching events paradigm. Launching events are the seeming collision and seeming transfer of movement between two objects—abstract, featureless stimuli (“objects”) in Michotte’s original experiments. Here, we study the relation between causal ratings for launching events in Michotte’s setting and launching collisions in a photorealistically computer-rendered setting. We presented launching events with differing temporal gaps, the same launching processes with photorealistic billiard balls, as well as photorealistic billiard balls with realistic motion dynamics, that is, an initial rebound of the first ball after collision and a short sliding phase of the second ball due to momentum and friction. We found that providing the normal launching stimulus with realistic visuals led to lower causal ratings, but realistic visuals together with realistic motion dynamics evoked higher ratings. Two-dimensional versus three-dimensional presentation, on the other hand, did not affect phenomenal causality. We discuss our results in terms of intuitive physics as well as cue conflict.

Thermodynamic Analysis of Flow Field at the End of Combustor Simulator

2012 ◽  
Vol 225 ◽  
pp. 261-266 ◽  
Author(s):  
Kianpour Ehsan ◽  
Nor Azwadi Che Sidik ◽  
Mohsen Agha Seyyed Mirza Bozorg
Keyword(s):  
Flow Field ◽  
Penetration Depth ◽  
Thermodynamic Analysis ◽  
Film Cooling ◽  
Thermal Field ◽  
Three Dimensional ◽  
Leading Edge ◽  
Aero Engine ◽  
Three Dimensional Presentation

This study was carried out to investigate the effects of different cooling holes configurations on the thermal field characteristics inside a combustor simulator. In this research, a three-dimensional presentation of a true Pratt and Whitney aero-engine was simulated and analyzed. This combustor simulator combined the interaction of two rows of dilution jets, which were staggered in the stream wise direction and aligned in the span wise direction. The findings of the study indicate that the thickness of the film-cooling layer was thicker for the greater penetration depth. Furthermore, for the combustor simulator with more cooling holes, the temperature near the wall and between the jets was slightly increased. Also at the leading edge of the jet, the gradients of temperature were quite high at the jet-mainstream interface.

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