Endophytic Fungi: Role in Phosphate Solubilization

Root endophytic fungi plays different roles for plant, such as plant growth promoting properties, agents to control phytopathogens, and increase phosphorus uptake. Since phosphorus are essential for plant growth and its occurance are limited, so it is necessary to explore these fungus to replace the used of synthetic fertilizer. The objective of this study were to obtain root endophytic fungi from rice plant and to determine its phosphate solubilization ability. The root organ of rice plant was subjected for isolation. Pikovskaya medium was use to determine the fungal phosphorus solubilization ability. Fungal morphological characteristics was carried out by macroscopic and microscopic appearance assessment using microscope. Seven endophytic fungi were successfully isolated from rice plant root sample. Four isolate were micelial steril with no conidia, two isolate refers to Aspergillus and 1 isolate have 2-4 conidia at the tip of conidiophores. Amongs 7 endophytics fungi, only one isolate (P2B3) had the ability to solubilize phosphate with the phosphate solubilization index value 20.45 %.

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ISOLATION AND PRELIMINARY SCREENING OF ENDOPHYTIC FUNGI FROM Ficus carica FOR BIOCONTROL AND PHOSPHATE SOLUBILIZATION

Environment & Ecosystem science ◽

10.26480/ees.02.2020.77.84 ◽

2020 ◽

Vol 4 (2) ◽

pp. 77-84

Author(s):

Norsalsabila Mohd Rosli ◽

Khairul Izzah Abu Hasan Ashari ◽

Nur Sabrina Ahmad Azmi

Keyword(s):

Endophytic Fungi ◽

Phosphate Solubilization ◽

Fusarium Verticillioides ◽

Fungal Endophytes ◽

Ficus Carica ◽

Biotic And Abiotic Stress ◽

Mycelium Growth ◽

Preliminary Screening ◽

The Family ◽

Phosphate Solubilizing

Fungal endophytes are a special group of symbionts that invade the internal environment of the host organism without causing any pathogenic symptoms. They increase the vigor of the plant while protecting them from several biotic and abiotic stress conditions such as herbivory, disease, and drought. In this study, endophytic fungi were isolated from fig (Ficus carica), a beneficial plant belongs to the family of Moraceae. This study was conducted to determine the potential of fungal endophytes of F.carica as biocontrol agents against selected phytopathogens Ganoderma boninense, Magnaporthe oryzae, and Fusarium verticillioides. Besides, endophytic fungi isolates were also screened to assess their ability as a phosphate-solubilizing agent. Results obtained revealed that a total of 11 fungal endophytes was successfully isolated from leaf, root, and stem samples of F.carica. The cultural-morphological identifications were carried out on the unknown fungal isolates. For the antagonistic test, S2-1 and R3-4 show a promising potential against those phytopathogens with more than 20% Percentage Inhibition Radial Growth (PIRG). Endophyte S2-1 exhibits the competition mechanism, while R3-4 expresses the antibiosis mechanism in suppressing the mycelium growth of phytopathogens. As for phosphate solubilization, 8 of 10 isolates show positive results as phosphate solubilizer with the highest PSI value 3.02±0.05 (S2-4).

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Biochemical characterization of multifunctional endophytic fungi from Bambusa oldhamii Munro

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632020v5066370 ◽

2020 ◽

Vol 50 ◽

Author(s):

Andreia Alves da Costa Silveira ◽

Leila Garcês de Araújo ◽

Marta Cristina Corsi de Filippi ◽

Fabrícia Paula de Faria ◽

Sérgio Tadeu Sibov

Keyword(s):

Acetic Acid ◽

Endophytic Fungi ◽

Indole Acetic Acid ◽

Biochemical Characterization ◽

Phosphate Solubilization ◽

Growth Promotion ◽

Future Studies ◽

Polyphenol Oxidases ◽

Bambusa Oldhamii

ABSTRACT In a plant-endophyte relationship, substances secreted by microorganisms can help the plant to defend itself against pathogens and also to promote an increase in biomass. This study aimed to biochemically characterize endophytic fungi from Bambusa oldhamii Munro, aiming at future studies on plant growth promotion, as well as on resistance induction. The following fungal isolates from B. oldhamii were used: 29 (Acrocalymma sp.); 122 (Botryobambusa fusicoccum); 711 (Phoma sp.); and 712 (Phoma sp.). Biochemical characterization was performed, analyzing the production of indole-acetic acid, chitinases and glucanases, as well as the presence or absence of polyphenol oxidases and phosphate solubilization activity in vitro. The isolate 29 produced larger amounts of indole-acetic acid and chitinases, yielding 31.55 mg mL-1 after 96 h and 2.59 U mL-1 after 120 h, respectively. The isolate 122 produced an increased amount of glucanases, yielding 2.65 U mL-1 after 72 h. The isolates 29, 711 and 712 produced polyphenol oxidases. The isolate 711 showed phosphate solubilization activity in vitro. These results indicate that the isolates under study show a potential for inducing resistance in plants and for future studies on the increase of plant biomass.

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