Allelic Combination | ScienceGate

Earliness, morphological and agronomic characters and grain quality were studied in 123 European landraces and old cultivars of winter wheat in three-year field experiments. Simultaneously, HMW Glu-alleles were identified in these cultivars by means of SDS-PAGE. Within this set of cultivars 224 Glu-lines (with occurrence over 5% in the cultivar) were identified carrying 3 different allelic combinations at 1A, 10 combinations at 1B and 3 combinations at 1D chromosomes, respectively. Relatively rare were alleles 2* at 1A and 3+12 at 1D as well as alleles 8, 6, 9, 7, 13+16 and 17+ 18 at 1B. Allele 20 at 1B was identified only in cultivars from DNK, CHE and EST. Allele 2* at 1A locus was found mainly in cultivars from Eastern, South-Eastern and Central Europe. Allelic combination 17+18 at 1B was also characteristic of cultivars from Central Europe. However, the gluten patterns themselves were not a sufficient tool for geographic characterisation of cultivars. The composition of Glu-alleles influenced the earliness of cultivars (alleles 2* at 1A, 17+ 18 and 6 at 1B and 3+12 at 1D). Spike length was positively affected by allele 1 at 1A and number of spikelets per spike by alleles 2+12 et 1D chromosome. Allele 2* was also associated with lower grain weight per spike. Crude protein content was decreased in cultivars where GS at 1A locus was absent (0). The value of gluten index was considerably higher (59.2) in cultivars bearing allelic combination 5+10 at 1D. A number of alleles affected the values of SDS micro-sedimentation test.

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Parental influences on X chromosome expression

Genetics Research ◽

10.1017/s0016672300021972 ◽

1985 ◽

Vol 45 (1) ◽

pp. 95-100 ◽

Cited By ~ 11

Author(s):

Lesley M. Forrester ◽

J. D. Ansell

Keyword(s):

X Chromosome ◽

Phosphoglycerate Kinase ◽

Parental Influences ◽

Allelic Combination ◽

The Relationship ◽

Maternal Imprinting

SummaryUsing mice that were mosaics for both Xce and phosphoglycerate kinase (Pgk-1) alleles, we have established that the parental source of the Xce gene may affect the probability with which the X chromosome carrying it will remain active. This effect was seen in one allelic combination of Xce but not in another. The relationship between these effects and other phenomena of maternal ‘imprinting’ is discussed.

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Varying abundance and dispersal of the two-spotted spider mite (Tetranychus urticae Koch, 1836, Acari: Prostigmata: Tetranychidae) on Mi-tomato plants differing in allelic combination

Biological Letters ◽

10.2478/v10120-011-0022-8 ◽

2011 ◽

Vol 48 (2) ◽

pp. 213-223 ◽

Cited By ~ 1

Author(s):

Monika Godzina ◽

Małgorzata Kiełkiewicz ◽

Katarzyna Szymczykiewicz

Keyword(s):

Tetranychus Urticae ◽

Spider Mite ◽

Dominant Gene ◽

Nematode Species ◽

Spider Mites ◽

Root Knot Nematode ◽

Tomato Plants ◽

Allelic Combination ◽

Two Spotted Spider Mite ◽

Abundance And Distribution

Varying abundance and dispersal of the two-spotted spider mite (Tetranychus urticaeKoch, 1836, Acari: Prostigmata: Tetranychidae) onMi-tomato plants differing in allelic combinationIt is well known that the presence of theMi-1.2gene determines the resistance of tomato plants (Solanum lycopersicumL.) to 3 root-knot nematode species and some insect herbivores of the order Hemiptera. In this study, the density and dispersal of two-spotted spider mites (Tetranychus urticae) on tomato plants of 4 cultivars were evaluated under greenhouse conditions, to find out whether tomatoes differing in allelic combination of theMi-1.2gene exhibit similar or different resistance to the mite pest. The results show, for the first time, that the same initial number of mites develops into populations that vary in abundance and distribution depending on tomato allelic composition of theMi-1.2gene. The results indicate that the mite-pest develops more slowly on tomato plants of cultivar ‘Motelle’, carrying 2 dominant alleles for theMi-1.2gene, than on heterozygous ‘Altess F1’ tomatoes and both cultivars carrying only recessive alleles of this gene: ‘Moneymaker’ and ‘Plaisence F1’. This suggests that theMi-1.2gene may be involved in tomato resistance against spider mites when this dominant gene is expressed homozygously, but this needs to be verified by further research.

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Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley

Czech Journal of Genetics and Plant Breeding ◽

10.17221/90/2010-cjgpb ◽

2010 ◽

Vol 46 (No. 4) ◽

pp. 149-158 ◽

Cited By ~ 2

Author(s):

L. Holková ◽

P. Mikulková ◽

P. Hrstková ◽

I.T. Prášil ◽

M. Bradáčová ◽

...

Keyword(s):

Genetic Linkage ◽

Segregation Analysis ◽

Growth Habit ◽

Frost Tolerance ◽

Allelic Combination ◽

Winter Type ◽

Spring Variety ◽

Allelic Variations ◽

Derivatives Of ◽

Allele Combination

The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from –14.2°C to –15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT50 from –10.0°C to –14.3°C). The genetic linkage between Dhn4 and Dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F2 plants from a cross between two genotypes carrying different allelic combination of Dhn4 and Dhn7 genes (two-rowed spring variety Akcent × six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant.

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Relevance of the Mi23 Marker and the Potato Aphid Biology as Indicators of Tomato Plant (Solanum lycopersicum L.) Resistance to Some Pests

Vegetable Crops Research Bulletin ◽

10.2478/v10032-010-0003-1 ◽

2010 ◽

Vol 72 (1) ◽

pp. 25-33 ◽

Cited By ~ 4

Author(s):

Monika Godzina ◽

Mirosława Staniaszek ◽

Małgorzata Kiełkiewicz

Keyword(s):

Solanum Lycopersicum ◽

Tomato Plant ◽

Macrosiphum Euphorbiae ◽

Tomato Plants ◽

Potato Aphid ◽

Allelic Combination ◽

Solanum Lycopersicum L ◽

Biological Potential ◽

Recessive Locus ◽

Tomato Cultivars

Relevance of the Mi23 Marker and the Potato Aphid Biology as Indicators of Tomato Plant (Solanum lycopersicumL.) Resistance to Some PestsMi-1.2gene, expressed in tomato plants, contributes to endogenous resistance against nematodes and someHemipterainsects. The aim of this study was to screen the presence of dominant/recessive locus of theMi-1.2gene in tomato cultivars with different allelic combination using Mi23 SCAR method and to assess the capacity of the local potato aphid (Macrosiphum euphorbiaeThomas) population to develop on different tomato cultivars (dominant and recessive homozygotes inMi-1.2locus). The results showed that both Mi23 marker and potato aphid performance are relevant methods in screening tomato cultivars with a different allelic combination ofMi-1.2gene. The assessment of biological potential ofM. euphorbiaeproved that, in comparison with control (tomato plants with recessive alleles ofMi-1.2gene), the aphid mortality increased 9- and 4 - fold (in the first and second experimental series, respectively) and the female longevity decreased 3 - fold when fed on tomato cvs with dominant alleles ofMi-1.2gene. Furthermore, the resistance against aphids manifests as an antibiosis mechanism in tomato plants carrying dominant alleles.

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Arthritis in MRL/lpr mice is under the control of multiple gene loci with an allelic combination derived from the original inbred strains

Arthritis & Rheumatism ◽

10.1002/art.10193 ◽

2002 ◽

Vol 46 (4) ◽

pp. 1067-1074 ◽

Cited By ~ 36

Author(s):

Junji Kamogawa ◽

Miho Terada ◽

Shinichi Mizuki ◽

Miyuki Nishihara ◽

Haruyasu Yamamoto ◽

...

Keyword(s):

Inbred Strains ◽

Multiple Gene ◽

Allelic Combination

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Emerging ofptxP3 lineage inBordetella pertussis strains circulating in a population in northeastern Mexico

Epidemiology and Infection ◽

10.1017/s0950268818002303 ◽

2018 ◽

Vol 146 (16) ◽

pp. 2096-2101 ◽

Cited By ~ 2

Author(s):

J. L. Gutiérrez-Ferman ◽

L. Villarreal-Treviño ◽

J. M. Ramírez-Aranda ◽

A. Camacho-Ortiz ◽

M. R. Ballesteros-Elizondo ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Chain Reaction ◽

Pertussis Infection ◽

Allelic Combination ◽

Global Spread ◽

Northeastern Mexico ◽

Polymerase Chain ◽

Potential Impact

AbstractWe determined the molecular epidemiology ofBordetella pertussisisolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). SelectedB. pertussisisolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) andptxP-ptxA, prn,fim2 andfim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A,n= 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combinationptxP3-ptxA1.The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypesptxP3-ptxA1-prn2-fim2-1-fim3-2 andptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypesptxP3-ptxA1-prn2-fim2-1-fim3-2andptxP3-ptxA1-prn2-fim2-1-fim3-1were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

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Cytotoxic T lymphocyte-Associated Antigen +49G Variant Confers Risk for Anti-CCP- and Rheumatoid Factor-Positive Type of Rheumatoid Arthritis Only in Combination with CT60∗G Allele

Autoimmune Diseases ◽

10.4061/2010/285974 ◽

2010 ◽

Vol 2010 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Bernadett Farago ◽

Peter Kisfali ◽

Lili Magyari ◽

Noemi Polgar ◽

Bela Melegh

Keyword(s):

Rheumatoid Arthritis ◽

Regression Analysis ◽

Rheumatoid Factor ◽

T Lymphocyte ◽

Cytotoxic T Lymphocyte ◽

Healthy Controls ◽

Predisposing Factor ◽

Positive Type ◽

Allelic Combination

Controversial observations have been published on the association of the cytotoxic T lymphocyte associated antigen gene's variants with rheumatoid arthritis (RA). After genotyping 428 patients and 230 matched controls, the prevalence of theCT60∗G allele was more frequent in RF- and/or anti-CCP-seropositive RApatients, compared to the healthy controls (P<.001). Regression analysis revealed that theCT60∗G allele is a possible predisposing factor for RA in these subgroups. No accumulation of the+49∗G allele was found among patients, and this variant was not found to correlate with RA. Assaying the possible genotype variations, the+49∗G-CT60∗G allelic combination was accumulated in seropositive RA-subtypes, and was associated with the risk of RA (OR=1.73,P=.001for the whole RA-population). Although the+49∗G allele did not mean a predisposition to RA alone, in combination withCT60∗G it, also conferred risk, suggesting that the+49A/Gvariant is associated with the risk of RA only in certain haplotypes.

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PPARG-LYPLAL1 Multi-Allelic Combination Associated with Obesity and Overweight in Mexican Adolescent Females

Ethnicity & Disease ◽

10.18865/ed.26.4.477 ◽

2016 ◽

Vol 26 (4) ◽

pp. 477

Author(s):

Esther-Alhelí Hernández-Tobías ◽

Luisa Torres-Sánchez ◽

Gino Noris ◽

Carla Santana ◽

Maria Reyna Samano ◽

...

Keyword(s):

Metabolic Disorders ◽

Caloric Intake ◽

Normal Weight ◽

World Health ◽

Allelic Combination ◽

Risk Alleles ◽

Obesity And Overweight ◽

Confounding Variables ◽

Population Bias ◽

Health Organization

Objective: We studied multi-loci variants to identify the contribution of six candidate genes (ADIPOQ, CDH13, LYPLAL1, MC4R, PPARG and PGC1A) in the development of obesity and overweight.Design: We genotyped 404 chromosomes with eleven SNPs in Mexican female adolescents, who were subdivided into two groups (obesity-overweight and normal-weight) using the World Health Organization parameters. Genomic (800 chromosomes) and ancestral (208 chromosomes) controls were included to reduce the population bias. Anthropometric measurements, biochemical parameters, and caloric intake were obtained only in the groups of Mexican female adolescents.Results: A positive genotype-phenotype association was found that involves the multi-allelic combination of three risk alleles (one in PPARG and two in LYPLAL1) with obesity and overweight (OR=3.1, P=.010). This combination also exhibited a significant association with waist circumference (P=.030) and triglycerides levels (P=.030). These associations were supported by a logistic regression analysis adjusted for several confounding variables.Conclusions: Our data suggest the joint participation of PPARG-LYPLAL1 genes in metabolic disorders development. Hence, these genes could act as potential biomarkers in obesity and overweight. Our findings underscore the complexity of metabolic disorders and provide evidence about the importance of multi-loci analysis to study complex diseases.Ethn Dis. 2016;26(4):477-484; doi:10.18865/ ed.26.4.477

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Evaluation of variants in IL6R, TLR3, and DC-SIGN genes associated with dengue in sampled Colombian population

Biomédica ◽

10.7705/biomedica.v39i1.4029 ◽

2019 ◽

Vol 39 (1) ◽

pp. 88-101 ◽

Cited By ~ 2

Author(s):

Efren Avendaño-Tamayo ◽

Alex Rúa ◽

María Victoria Parra-Marín ◽

Winston Rojas ◽

Omer Campo ◽

...

Keyword(s):

Dengue Fever ◽

Odds Ratio ◽

Fragment Length Polymorphism ◽

African Ancestry ◽

Influential Factor ◽

Chain Reaction ◽

Chi Square ◽

Allelic Combination ◽

Increased Risk ◽

Polymerase Chain

Introduction: Host genetics is recognized as an influential factor for the development of dengue disease.Objective: This study evaluated the association of dengue with the polymorphisms rs8192284 for gene IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN.Materials and methods: Of the 292 surveyed subjects, 191 were confirmed for dengue fever and the remaining 101 were included as controls. The genotypes were resolved using polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP). In an attempt to determine the risk (Odds Ratio) of suffering dengue fever, data were analyzed using chi-square for alleles and logistic regression for both genotypes and allelic combinations. Confidence intervals were set to 95% for all tests regardless of the adjustment by either self-identification or ancestry.Results: For Afro-Colombians, the allele rs8192284 C offered protection against dengue [OR=0.425,(0.204-0.887), p=0.020]. The alleles rs7248637 A and rs3775290 A posed, respectively, an increased risk of dengue for Afro-Colombians [OR=2.389, (1.170-4.879), p=0.015] and Mestizos [OR=2.329, (1.283-4.226), p=0.005]. The reproducibility for rs8192284 C/C [OR=2.45, (1.05-5.76), p=0.013] remained after adjustment by Amerindian ancestry [OR=2.52, (1.04-6.09), p=0.013]. The reproducibility for rs3775290 A/A [OR=2.48, (1.09-5.65), p=0.033] remained after adjustment by European [OR=2.34, (1.02-5.35), p=0.048], Amerindian [OR=2.49, (1.09-5.66), p=0.035], and African ancestry [OR=2.37, (1.04-5.41), p=0.046]. Finally, the association of dengue fever with the allelic combination CAG [OR=2.07, (1.06-4.05), p=0.033] remained after adjustment by Amerindian ancestry [OR=2.16, (1.09-4.28), p=0.028].Conclusions: Polymorphisms rs8192284 for IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN were associated with the susceptibility to suffer dengue fever in the sampled Colombian population.

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