scholarly journals Diversity of HMW-Glu Alleles and Evaluation of their Effects on some Characters in Winter Wheat Landraces and Old Cultivars

2012 ◽  
Vol 38 (No. 3-4) ◽  
pp. 109-116
Author(s):  
L. Dotlačil ◽  
E. Gregová ◽  
J. Hermuth ◽  
Z. Stehno ◽  
J. Kraic

Earliness, morphological and agronomic characters and grain quality were studied in 123 European landraces and old cultivars of winter wheat in three-year field experiments. Simultaneously, HMW Glu-alleles were identified in these cultivars by means of SDS-PAGE. Within this set of cultivars 224 Glu-lines (with occurrence over 5% in the cultivar) were identified carrying 3 different allelic combinations at 1A, 10 combinations at 1B and 3 combinations at 1D chromosomes, respectively. Relatively rare were alleles 2* at 1A and 3+12 at 1D as well as alleles 8, 6, 9, 7, 13+16 and 17+ 18 at 1B. Allele 20 at 1B was identified only in cultivars from DNK, CHE and EST. Allele 2* at 1A locus was found mainly in cultivars from Eastern, South-Eastern and Central Europe. Allelic combination 17+18 at 1B was also characteristic of cultivars from Central Europe. However, the gluten patterns themselves were not a sufficient tool for geographic characterisation of cultivars. The composition of Glu-alleles influenced the earliness of cultivars (alleles 2* at 1A, 17+ 18 and 6 at 1B and 3+12 at 1D). Spike length was positively affected by allele 1 at 1A and number of spikelets per spike by alleles 2+12 et 1D chromosome. Allele 2* was also associated with lower grain weight per spike. Crude protein content was decreased in cultivars where GS at 1A locus was absent (0). The value of gluten index was considerably higher (59.2) in cultivars bearing allelic combination 5+10 at 1D. A number of alleles affected the values of SDS micro-sedimentation test.

2011 ◽  
Vol 42 (No. 2) ◽  
pp. 50-57 ◽  
Author(s):  
V. Dvořáček ◽  
J. Bradová ◽  
Z. Stehno

A set of quality parameters (crude protein content, Zeleny sedimentation test, wet gluten content, gluten index, albumins + globulins content, gliadin content, sum of glutenins, proportion of albumins + globulins in crude protein and relative viscosity) was tested in 17 doubled haploid (DH) wheat lines differing in the presence or absence of 1B/1R translocation. The presence of 1B/1R translocation (allele Gli 1B3) affected the significantly higher content of albumins and globulins and significantly lower value of gluten index. Nevertheless, a certain translocation influence on the other parameters (Zeleny sedimentation test; relative viscosity; proportion of albumins + globulins in crude protein) was also registered. The deteriorative effect of the 1B/1R translocation on indirect technological grain parameters was confirmed more markedly in gluten index than in Zeleny sedimentation test. A high number of significant differences between lines was found in Zeleny sedimentation test, content of glutenin, content of albumins + globulins and their proportion in crude protein. In spite of lower variability between lines relative viscosity showed a high dependence on genotype and was indifferent to the other quality parameters.    


2016 ◽  
Vol 11 (1) ◽  
pp. 22-26
Author(s):  
Золоторева ◽  
Rimma Zolotoreva ◽  
Виноградов ◽  
Georgiy Vinogradov ◽  
Максимов ◽  
...  

The article discusses the influence of mineral fertilizers on formation of productivity and grain quality of different spring barley types in the soil and climatic conditions of the Republic of Mari El. Research has established, that the application of mineral fertilizers for spring barley in doses of N60P60K60 and N90P60K60 increases the crude protein content in the grain and increase harvesting of feed units per unit of cultivated area.


2020 ◽  
Vol 15 (1) ◽  
pp. 79-88
Author(s):  
Yu. A. Gulyanov ◽  
A. A. Chibilyov ◽  
A. A. Chibilyov Jr.

Aim. Verification of scientific concepts regarding the spatial heterogeneity of field agrocenoses. Identification of the variability of phytometric and structural crop ndicators and determination of the degree of their influence on the yield and quality of winter wheat grain in the steppe zone of the Orenburg Urals.Material and Methods. Establishment of field experiments, related observations and counts in accordance with the methodology of state variety crops testing and B.A.Dospekhov's guidelin. Monitoring of winter wheat crops was carried by measuring the vegetation index (NDVI) with a Green Seeker Handheld Crop Sensor, Model HCS‐100 (Trimble, USA). Determination of grain quality indicators was conducted according to GOST 9353‐2016 Wheat – Technical Conditions. Microsoft Office Excel was employed for the correlation and regression analysis of experimental data. Results. Analysis of the intra‐field heterogeneity of winter wheat agrocenoses in terms of yield and grain quality was conducted. The dependences of yield and grain quality on the principal crop phytometric and structural parameters were defined and expressed in the form of regression equations.Сonclusion. The results of the studies attest to the growth of reserves of grain yield to 3.0 t/ha and grain quality to class I‐II class in zonal climatic conditions of optimization of environmental factors to the level of the best basic plots by levelling out field soil heterogeneity. This is possible by restoring the fertility of anthropogenically‐degraded soil through the introduction of landscape‐adaptive and resource saving farming systems, soil protective and soil restorative crop rotation, differentiated application of organic and mineral fertilizers and selection of the most adaptive varieties. We also advise the introduction of intelligent ‘digital technologies’ aimed at fuller implementation of the genetic potential of cultivated varieties with careful consideration of natural resources and the preservation of biological diversity.


2021 ◽  
Vol 182 (3) ◽  
pp. 54-62
Author(s):  
N. F. Sintsova ◽  
I. V. Lyskova ◽  
E. I. Kratyuk ◽  
V. M. Arkhipov

Background. Resistance of raw and boiled tuber flesh to darkening when potato is processed into potato-based products or consumed directly is an important requirement for new potato cultivars. Identification of hybrids resistant to tuber flesh darkening would contribute immensely to future breeding work.Materials and methods. Parent forms and hybrid progeny of potato were tested for five years (2016–2020) in Kirov Province using the methods and technologies approved for potato breeding process. The progeny of 10 cultivars used as pollinators was analyzed.Results and conclusions. Cultivars with high levels of resistance to the darkening of raw tuber flesh were selected in the collection nursery. A total of 62 accessions (or 24%) out of 256 were identified for the absence of darkening or only small traces thereof (8 points) in raw tubers. Medium and strong darkening of the flesh (4–5 points) was recorded for 45 accessions. Analyzing the progeny of pollinator cultivars showed an increased occurrence of hybrids resistant to tuber flesh darkening in the progeny of cv. ‘Manifest’: 71% of the studied hybrids had non-darkening flesh (9 points). Manifestation of tuber flesh darkening was found to depend on weather conditions during the growing season: the higher was the air temperature, the weaker was the darkening of raw tuber flesh, but when the soil moisture during tuberization was high, tuber flesh became more darkened. A statistically significant relationship was observed between the darkening of boiled tubers and the content of crude protein (r = 0.74) and vitamin C (r = 0.79). Hybrid 179-10 was identified for high productivity (32.5 t/ha) and crude protein content (1.54%), while the highest resistance to the darkening of raw and boiled potato tubers was registered in hybrid 72-13.


1988 ◽  
Vol 60 (4) ◽  
pp. 307-321
Author(s):  
Matti Rekunen

From 1977 to 1987, variety trials were conducted at two test sites in order to determine the agronomic value of old oat varieties as compared to that of the varieties presently grown. The trial sites were located at two breeding stations of the Hankkija Plant Breeding Institute, those of Nikkilä (61° 33' N latitude) and Anttila (60° 25' N latitude). The variety trials were performed according to the methods of Partially Balanced Lattices, and the results and their summary were computed according to the Nearest Neighbour Analysis. Eleven oat varieties released by the Institute between 1921 and 1982 as well as the old native oat variety from Ylitornio and the American oat variety Wasa, released in the 1920s, were all tested simultaneously. The materials are presented in Table 1, the test environment in Table 2. The main findings are shown in Tables 3 & 4 and in Figures 1—5. The correlations between the age of the varieties and their characteristics are presented, in order of quality, in Table 5. The field experiments showed that intense progress inbreeding work is reflected as increased crop yield, shortening of the straw, improved strength of the straw, and decreased husk content. In the trials the crop yield of the newest variety, Hankkijan Vouti variety, averaged 51 per cent more than the Ylitornio land race variety. The mean improvement in these properties achieved in 1921—-1982by means of breeding work was: an increase in crop yield of approximately 40 per cent; shortening of the straw by roughly 20 per cent; reduction of the tendency to lodging by about half; and lowering of the husk content by some 2 percentage points. In contrast, no constant advances could be shown for the earliness at heading or ripening, the 1 000 kernel weight, the hectolitre weight, the crude protein content or resistance to Barley Yellow Dwarf Virus (BYDV). The 1 000 kernel weight and the hectolitre weight had risen from those of the local oat variety, whereafter development ceased to occur. The study also investigated the ratio between genotype and environmental effects. In the experimental series, environmental factors had an effect on determining the time needed for ripening, the crude protein content, the length of the straw, the hectrolitre weight and crop yield that was at least five times greater than the effect of genotype. Genotype proved to have a stronger influence on the 1 000 kernel weight, for which the variance component of genotype was three fold that of the environmental component. The heritability values in the experimental series ranged from 0.19 (for BYDV infection) to 0.77 (for time to heading).


2011 ◽  
Vol 49 (No. 2) ◽  
pp. 67-74 ◽  
Author(s):  
L. Dotlačil ◽  
J. Hermuth ◽  
Z. Stehno

European winter wheat landraces and obsolete cultivars (121 accessions in set I and 101 accessions in set II) with modern check cultivars were evaluated in three-year field trials. Increased spike productivity in modern cultivars could be attributed mainly to increased number of grains in spikelet and increased HI, whereas TGW has marginal effect. Old cultivars had on average by 2–3% higher crude protein content in grain than modern ones. Among selected 10 characters, relatively wide diversity (C.V. 11–20%) has been estimated in spike length and characters of spike productivity (except of grain weight with C.V. close to 9%). It was difficult to distinguish the cultivars according to the country of origin, however, earliness and lower spike productivity seems to be characteristic for South-East origin whereas cultivars from North-West Europe showed opposite characters. Correlation analyses showed close relations between earliness in heading and in maturity and negative relation between late heading and grain filling period, which was positively correlated with TGW and HI (r = 0.26 to 0.38). Number of grains in spikelets was highly correlated with spike productivity and HI (r = 0.62 to 0.69) whereas relations between these two characters and TGW were lower (r = 0.20 to 0.51). Spike productivity characters, except of TGW, are in negative correlation with crude protein content in grain (r = –0.34 to –0.50). Regression analyses confirmed that main determining character for the spike productivity is number of kernels in spikelet (about 40% of variation) while effects of TGW are about half-size. Crude protein content was positively affected by plant height (15–30% of variation) impact of grain weight per spike was lower (14–17% of variation) and negative. Potentially valuable donors of earliness and longer grain filling period were identified for further studies and/or utilization in breeding programs. As especially valuable character can be considered very high crude protein content (around 18% in cvs Berg-land, Ukrajinka, Sippbachzeller, Innichen Nr. 25001 and Barbu du Finistre). High crude protein content combined with relatively good spike productivity and/or long grain filling period or earliness was found in cvs Visperterminen 640 E, Hatvan, Szekacz 1242, Berchtesgardener Vogel, Ble du Lot and Barbu du Finistere.


2012 ◽  
Vol 58 (No. 11) ◽  
pp. 514-520 ◽  
Author(s):  
H. Zhang ◽  
T.Q. Song ◽  
K.L. Wang ◽  
G.X. Wang ◽  
H. Hu ◽  
...  

Non-destructive and rapid monitoring methods for crude protein content (CPC) in rice grain are of significance in nitrogen diagnosis and grain quality monitoring, and in enhancing nutritional management and use efficiency. In this study, CPC and canopy spectra in rice were measured based on rice field experiment. Key spectral bands were selected by principal component analysis (PCA) method, and the predicted models were built by multiple linear regressions (MLR), artificial neural network (ANN) and partial least squares regression (PLSR). The results showed that there is a significant correlation between CPC content and key spectral bands. The results of prediction for the three models were in order of PLSR > ANN > MLR with correlation values of 0.96, 0.92 and 0.90, respectively, for the validation data. Therefore, it is implied that CPC in rice (grain quality) could be estimated by canopy spectral data.  


Author(s):  
Z. Abudjaba Z. ◽  
◽  
M.A. Mazirov M.A. ◽  
N.S. Matyuk N.S. ◽  
I. Akbar I.

The article compares and analyzes the productivity and composition of nutrients of two different agricultural crops of corn variety Sinyu No. 29 and fodder sorghum Jinmu No. 1, cultivated in stubble crops after winter wheat using the same technology. sorghum Jinmu No. 1 has the property of regrowing after the first mowing, which allows you to harvest two crops and increase the overall yield per unit area. The harvest of green mass of fodder sorghum variety Jinmu No. 1 was 120.9% higher than that of maize variety Sinyu No. 29 and amounted to 169762.78 kg / ha and 78277.87 kg / ha, respectively. Similarly, compared to Sinu No. 29 maize, Jinmu No. 1 fodder sorghum has a higher yield, higher crude protein content, higher crude ash and higher water content, which means better silage quality. basis for the promotion and use of fodder sorghum.


Author(s):  
Magdaléna Lacko-Bartošová ◽  
Lucia Lacko-Bartošová ◽  
Petr Konvalina

The objective of this study was to evaluate the effect of cropping systems [integrated (INT) vs. organic (ORG)] and plant nutrition sources (synthetic in INT vs. approved organic in ORG) on analytical and rheological quality traits of winter wheat and its productivity. The results after 16 years of field experiments were evaluated. Pre-crop for winter wheat was N-fixing crop. Grain yield of winter wheat (6.8 t ha<sup>–1</sup> in INT, 6.5 t ha<sup>–1</sup> in ORG) did not differ significantly, while plant nutrition sources had an equal and positive effect on the yield. Crude protein quantity was higher in INT system by about 0.2%. The farinograph dough development time and dough stability were the longest for ORG and fertilised treatments. Mixolab quality indicators showed a clear distinction between ORG and INT systems and fertilisation in the protein and starch characteristics of the grain. The ORG reported longer Mixolab stability of the dough, mainly on fertilised treatment (8.8 min). Starch characteristics – torque C4 (amylolytic activity) and torque C5 (starch retrogradation) were higher for ORG system. Torque C2, protein weakening, was not affected by the cropping system. ORG system has the potential to achieve consistent, high-quality yields with significantly lower reliance on external inputs.


2010 ◽  
Vol 46 (Special Issue) ◽  
pp. S70-S74 ◽  
Author(s):  
L. Dotlačil ◽  
J. Hermuth ◽  
Z. Stehno ◽  
V. Dvořáček ◽  
J. Bradová ◽  
...  

Two sets of winter wheat landraces and obsolete cultivars were studied in three-year field experiments, and compared with 3 modern control cultivars. The higher spike productivity in modern cultivars could mainly be attributed to an increased number of grains in a spikelet and harvest index (HI), whereas thousand grain weight (TGW) has only a slight effect. Landraces and old cultivars proved to have a higher content of crude protein. Spike productivity characteristics, except for TGW, showed a negative correlation with the crude protein content in the grain. The number of kernels in a spikelet strongly affected the spike’s productivity, whereas the TGW has only half the effect. The mean yield of four modern cultivars was 51% higher than the mean yield of 31 landraces and obsolete cultivars. Regression analysis proved the much stronger response of modern cultivars to environment (<I>b </I>= 1.63), then was the response of old cultivars (<I>b </I>= 0.87). Different responses to environments were found within the set of 31 landraces, as well. We could also identify potentially valuable donors of earliness and winter hardiness among the old cultivars. High crude protein content (up to 18%), and other valuable quality characteristics, were rather frequent. In both sets, HMW <I>Glu</I>-subunits were described, and we have additionally studied 67 selected lines. Among them, 10 lines showed the crude protein content of 17.5% to 18.3% (where the gluten index and Zeleny test varied from 28.5 to 54.0 and 36.8 to 61.7, respectively). High values of all quality characteristics showed lines gained from the cultivars Mindeszentpusztai (HUN), Szekacz 19 (HUN), Bartweizen linie a (AUT), Viglašská červenoklasá (CZE), as well as some others.


Sign in / Sign up

Export Citation Format

Share Document