Genomics and Genetic Manipulation of Protozoan Parasites Affecting Farm Animals

2017 ◽  
pp. 413-438
Author(s):  
Carlos E. Suarez ◽  
Heba F. Alzan ◽  
Brian M. Cooke
Keyword(s):  
Genetic Manipulation ◽  
Farm Animals ◽  
Protozoan Parasites

Prospects for the molecular genetic manipulation of livestock

1992 ◽  
Vol 1992 ◽  
pp. 7-7
Author(s):  
Ian Wilmut
Keyword(s):  
Gene Transfer ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Target Population ◽  
Early Embryo ◽  
Animal Production ◽  
Farm Animals ◽  
Effective Scheme ◽  
Pharmaceutical Proteins ◽  
Repair Mechanisms

Molecular genetic manipulation can now be used to produce pharmaceutical proteins in the milk of farm animals. In the longer term this technology will be used to modify aspects of animal production, but it is not clear how many manipulations will be useful nor when such applications will become practicable. Improvements are required in all four aspects of an effective scheme for either gene transfer or targeting: it must be possible 1). to make the change, 2). to regulate expression of the gene in the desired manner, 3). to identify genes that are able to have a significant effect and 4). to disseminate the change into the target population.There is only one method that has been used to transfer a gene in livestock and this depends upon injection of a few hundred copies of the gene into a nucleus in the early embryo. It is believed that integration occurs because the act of injecting fluid causes breakages in chromosomes and that the repair mechanisms sometimes include injected DNA. There are serious implications.

scholarly journals A Review of Some Protozoan Parasites Causing Infertility in Farm Animals

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
B. Y. Kaltungo ◽  
I. W. Musa
Keyword(s):  
Farm Animals ◽  
Protozoan Parasites

scholarly journals Generation of Genetically Engineered Livestock Using Somatic Cell Nuclear Transfer

Reproduction ◽  
2021 ◽  
Author(s):  
Irina Polejaeva
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Genetic Manipulation ◽  
Embryonic Stem ◽  
Genetically Engineered ◽  
Farm Animals ◽  
Technological Advancement ◽  
Genetic Mosaicism ◽  
Low Efficiency

Genetic engineering (GE) of livestock initially has been accomplished primarily using pronuclear microinjection into zygotes (1985 – 1996). The applications of technology were limited due to low integration efficiency, aberrant transgene expression resulting from random integration and presence of genetic mosaicism in transgenic founder animals. Despite enormous efforts to established embryonic stem cells (ESCs) for domestic species, the ESC GE technology does not exist for livestock. Development of Somatic Cell Nuclear Transfer (SCNT) has bypassed the need in livestock ESCs and revolutionized the field of livestock transgenesis by offering the first cell-based platform for precise genetic manipulation in farm animals. For nearly two decades since the birth of Dolly (1996 – 2013), SCNT was the only method used for generation of knockout and knockin livestock. Arrival of CRISPRS/Cas9 system, a new generation of gene editing technology, gave us an ability to introduce precise genome modifications easily and efficiently. This technological advancement accelerated production of GE livestock by SCNT and reinstated zygote micromanipulation as an important GE approach. The primary advantage of the SCNT technology is the ability to confirm in vitro that the desired genetic modification is present in the somatic cells prior to animal production. The edited cells could also be tested for potential off-target mutations. Additionally, this method eliminates the risk of genetic mosaicism frequently observed following zygote micromanipulation. Despite its low efficiency, SCNT is a well-established procedure in numerous laboratories around the world and will continue to play an important role in the GE livestock field.

Metabolic engineering of CHO cells to prepare glycoproteins

2018 ◽  
Vol 2 (3) ◽  
pp. 433-442 ◽  
Author(s):  
Qiong Wang ◽  
Michael J. Betenbaugh
Keyword(s):  
Metabolic Engineering ◽  
Cho Cells ◽  
Genetic Manipulation ◽  
Chinese Hamster ◽  
Post Translational Modification ◽  
Effector Functions ◽  
Recombinant Glycoproteins ◽  
Production Platform ◽  
Chemical Inhibitors ◽  
Amino Acid Mutations

As a complex and common post-translational modification, N-linked glycosylation affects a recombinant glycoprotein's biological activity and efficacy. For example, the α1,6-fucosylation significantly affects antibody-dependent cellular cytotoxicity and α2,6-sialylation is critical for antibody anti-inflammatory activity. Terminal sialylation is important for a glycoprotein's circulatory half-life. Chinese hamster ovary (CHO) cells are currently the predominant recombinant protein production platform, and, in this review, the characteristics of CHO glycosylation are summarized. Moreover, recent and current metabolic engineering strategies for tailoring glycoprotein fucosylation and sialylation in CHO cells, intensely investigated in the past decades, are described. One approach for reducing α1,6-fucosylation is through inhibiting fucosyltransferase (FUT8) expression by knockdown and knockout methods. Another approach to modulate fucosylation is through inhibition of multiple genes in the fucosylation biosynthesis pathway or through chemical inhibitors. To modulate antibody sialylation of the fragment crystallizable region, expressions of sialyltransferase and galactotransferase individually or together with amino acid mutations can affect antibody glycoforms and further influence antibody effector functions. The inhibition of sialidase expression and chemical supplementations are also effective and complementary approaches to improve the sialylation levels on recombinant glycoproteins. The engineering of CHO cells or protein sequence to control glycoforms to produce more homogenous glycans is an emerging topic. For modulating the glycosylation metabolic pathways, the interplay of multiple glyco-gene knockouts and knockins and the combination of multiple approaches, including genetic manipulation, protein engineering and chemical supplementation, are detailed in order to achieve specific glycan profiles on recombinant glycoproteins for superior biological function and effectiveness.

Drug-Induced Agranulocytosis: A Mini Review

2016 ◽  
Vol 2 (1) ◽  
pp. 57-59
Author(s):  
Pavithra D ◽  
Praveen D ◽  
Vijey Aanandhi M
Keyword(s):  
Bone Marrow ◽  
Complete Blood Count ◽  
Genetic Manipulation ◽  
White Blood Cells ◽  
Deep Infection ◽  
Morbidity Rate ◽  
Drug Induced ◽  
Serious Adverse Event ◽  
Mortality And Morbidity ◽  
The Individual

Agranulocytosis is also known to be granulopenia, causing neutropenia in circulating blood streams .The destruction of white blood cells takes place which leads to increase in the infection rate in an individual where immune system of the individual is suppressed. The symptoms includes fever, sore throat, mouth ulcers. These are commonly seen as adverse effects of a particular drug and are prescribed for the common diagnostic test for regular monitoring of complete blood count in an admitted patient. Drug-induced agranulocytosis remains a serious adverse event due to occurrence of severe sepsis with deep infection leading to pneumonia, septicaemia, and septic shock in two/third of the patient. Antibiotics seem to be the major causative weapon for this disorder. Certain drugs mainly anti-thyroid drugs, ticlopidine hydrochloride, spironolactone, clozapine, antileptic drugs (clozapine), non-steroidal anti-inflammatory agents, dipyrone are the potential causes. Bone marrow insufficiency followed by destruction or limited proliferative bone marrow destruction takes place. Chemotherapy is rarely seen as a causative agent for this disorder. Genetic manipulation may also include as one of the reason. Agranulocytosis can be recovered within two weeks but the mortality and morbidity rate during the acute phase seems to be high, appropriate adjuvant treatment with broad-spectrum antibiotics are prerequisites for the management of complicated neutropenia. Drugs that are treated for this are expected to change as a resistant drug to the patient. The pathogenesis of agranulocytosis is not yet known. A comprehensive literature search has been carried out in PubMed, Google Scholar and articles pertaining to drug-induced agranulocytosis were selected for review.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

Registration of animal rabies in Krasnodar region

2020 ◽  
pp. 3-4
Author(s):  
Oleg Yu. Chernykh ◽  
◽  
Vadim A. Bobrov ◽  
Sergey N. Zabashta ◽  
Roman A. Krivonos ◽  
...  
Keyword(s):  
Urban Form ◽  
Animal Species ◽  
Farm Animals ◽  
Natural Focus ◽  
Dead End ◽  
Wide Range ◽  
Epizootic Process ◽  
Krasnodar Region ◽  
Spread Of Infection ◽  
Reporting Data

Rabies remains a constant threat to humanity in many parts of the world. At the same time, scientifically grounded antiepizootic measures should be based on the peculiarities of the regional epizootology of this zooanthroponosis. The authors studied the epizootological and statistical reporting data of the Kropotkin Regional Veterinary Laboratory, presented an analysis of the registration of rabies in animals in Krasnodar region. From the obtained data, it should be noted that despite the wide range of animals involved in the epizootic process of rabies infection in Krasnodar region, dogs, cats and foxes play a major role in the reservation and spread of infection, which account for 78.6. Of the total number of registered cases, 15.5% falls on foxes, that indicates the natural focus of the disease, along with the manifestation of the disease in an urban form. At the same time, stray and neglected dogs and cats, which occupy a significant place among the total number of sick animals, are also sources and spread of the infection. Thus farm animals (8.3% of the total number of infected animals) are a biological dead end for the infection. Isolated cases of the disease were noted in muskrat, donkey, raccoon, raccoon dog, marten, ferret and jackal. The authors also established the specific morbidity of various animal species with rabies infection, that is an important aspect in the development and implementation of antiepizootic measures complex

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