Evaluation of BBB Damage in an UV Irradiation Model by Endogenous Protein Tracers

1994 ◽  
pp. 139-141
Author(s):  
J. V. Lafuente ◽  
J. Cervós-Navarro ◽  
E. Gutierrez Argandoña
Keyword(s):  
Uv Irradiation ◽  
Protein Tracers ◽  
Endogenous Protein

Effect of Thrombin on Phosphorylation of Platelet Membrane Proteins

1976 ◽  
Vol 35 (03) ◽  
pp. 635-642 ◽  
Author(s):  
M Steiner
Keyword(s):  
Protein Kinase ◽  
Kinase Activity ◽  
Qualitative Change ◽  
Protein Kinase Activity ◽  
Protein Substrates ◽  
Phosphoprotein Phosphatase ◽  
Progressive Loss ◽  
Platelet Membranes ◽  
Endogenous Protein ◽  
Considerable Loss

SummaryThe effect of thrombin on the phosphorylating activity of platelet membranes was compared to that of trypsin. Preincubation of non-32P phosphorylated platelet membranes with or without either of these two enzymes resulted in a considerable loss of membrane protein kinase activity which was most severe when trypsin was used. Protein kinase activity and endogenous protein acceptors decreased in parallel. 32P-phosphorylated membranes showed a slow but progressive loss of label which was accelerated by trypsin. Thrombin under these conditions prevented the loss of 32P-phosphate. These results are interpreted to indicate a thrombin-induced destruction of a phosphoprotein phosphatase. The protein kinase activity of phosphorylated platelet membranes using endogenous or exogenous protein substrates showed a significant reduction compared to non-phosphorylated membranes suggesting a deactivation of protein kinase by phosphorylation of platelet membranes. Neither thrombin nor trypsin caused a qualitative change in the membrane polypeptides accepting 32P-phosphate but resulted in quantitative alterations of their ability to become phosphorylated.

Wavelength-specific Activation of MAP Kinase Family Proteins by Monochromatic UV Irradiation¶

2001 ◽  
Vol 73 (2) ◽  
pp. 147 ◽  
Author(s):  
Yukihito Kabuyama ◽  
Miwako K. Homma ◽  
Masayuki Sekimata ◽  
Yoshimi Homma
Keyword(s):  
Map Kinase ◽  
Uv Irradiation ◽  
Kinase Family

The Study of Photoreversibility, Photopolimerization and Photocleavage Properties of Polyurethane Coumarin by Means of Electronic Spectrometry and AFM Methods

2017 ◽  
Vol 68 (7) ◽  
pp. 1632-1635
Author(s):  
Silviu Gurlui ◽  
Emil Buruiana ◽  
Ioan Gabriel Sandu ◽  
Mitachi Strat
Keyword(s):  
Uv Irradiation ◽  
Room Temperature ◽  
Dimethyl Formamide ◽  
Photon Excitation ◽  
Photochemical Properties ◽  
Film State

The spectroscopic and photochemical properties of polyurethane coumarin (PUC) in dimethyl sulf-oxide (DMSO), thetra hydro furan (THF), dimethyl formamide (DMF) and film state were investigated at room temperature under one photon excitation. The results show that under irradiation of l ] 310 nm photodimerization process are increased and under UV irradiation with l[ 260 nm, photocleavage of polymer have been evidenced, too.

The Influence of Some Factors on Spirolactone Stability in Solution

2008 ◽  
Vol 59 (7) ◽  
Author(s):  
Daniela Lucia Muntean ◽  
Silvia Imre ◽  
Cosmina Voda
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Uv Irradiation ◽  
High Performance ◽  
Degradation Products ◽  
Simultaneous Action ◽  
Ethanolic Solution ◽  
Degradation Processes ◽  
Stable Solutions ◽  
Preformulation Study

The influence of some factors on spironolactone stability in solution was studied, by applying high-performance liquid chromatography, as a part of a pharmaceutical preformulation study in order to obtain a spironolactone solution for alopecia treatment. Solutions of 1 mg/ml spironolactone in aqueous ethanolic solution 1 : 1 and in 20 mM cyclodextrines solutions (b-, hydroxi-b- and methyl-b-cyclodextrine) was used, maintained at 8 and 22 �C, protected from light and after UV irradiation at 254 nm. The main degradation products were 7a-thiospirolactone and canrenone. The most stable solutions were the alcoholic ones and with methyl-beta-cyclodextrine, but the simultaneous action of temperature and UV irradiation allowed degradation processes after one hour of exposure, more aggressive in the presence of methyl-beta-cyclodextrine. In conclusion, for alopecia treatment with spironolactone a 1 mg/mL ethanolic solution could be used and it is recommendable the protection of treated zone.

Sign in / Sign up

Export Citation Format

Share Document