Streptomyces sp.VSMGT1014-Mediated Antifungal Activity Against Fungal Plant Pathogens

2012 ◽  
pp. 335-341 ◽  
Author(s):  
H. Harikrishnan ◽  
V. Shanmugaiah
Keyword(s):  
Antifungal Activity ◽  
Plant Pathogens ◽  
Fungal Plant Pathogens

Antifungal activity of extracts from endophytic fungi associated with Smallanthus maintained in vitro as autotrophic cultures and as pot plants in the greenhouse

2012 ◽  
Vol 58 (10) ◽  
pp. 1202-1211 ◽  
Author(s):  
Luiz H. Rosa ◽  
Nurhayat Tabanca ◽  
Natascha Techen ◽  
Zhiqiang Pan ◽  
David E. Wedge ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Endophytic Fungi ◽  
Plant Pathogens ◽  
Cultivation Conditions ◽  
Smallanthus Sonchifolius ◽  
Fungal Plant Pathogens ◽  
In The Wild ◽  
Fungal Assemblages ◽  
Pot Plants

The endophytic fungal assemblages associated with Smallanthus sonchifolius (Poepp.) H. Rob. and Smallanthus uvedalius (L.) Mack. ex Small growing in vitro autotrophic cultures and in the greenhouse were identified and evaluated for their ability to produce bioactive compounds. A total of 25 isolates were recovered that were genetically closely related to species of the genera Bionectria , Cladosporium , Colletotrichum , Fusarium , Gibberella , Hypocrea , Lecythophora , Nigrospora , Plectosphaerella , and Trichoderma . The endophytic assemblages of S. sonchifolius presented a greater diversity than the group isolated from S. uvedalius and demonstrated the presence of dominant generalist fungi. Extracts of all fungi were screened against the fungal plant pathogens. Ten extracts (41.6%) displayed antifungal activities; some of them had a broad antifungal activity. The phylotypes Lecythophora sp. 1, Lecythophora sp. 2, and Fusarium oxysporum were isolated from in vitro autotrophic cultures and displayed antifungal activity. The presence of bioactive endophytic fungi within S. sonchifolius and S. uvedalius suggests an ecological advantage against pathogenic attacks. This study revealed reduced numbers of endophytes in association with both Smallanthus species in controlled cultivation conditions compared with the endophytic communities of hosts collected in the wild environments. Even as reduced endophytic communities, these fungi continue to provide chemical protection for the host.

scholarly journals Composition, Mosquito Larvicidal, Biting Deterrent and Antifungal Activity of Essential Oils of Different Plant Parts of Cupressus arizonica var. glabra (‘Carolina Sapphire’)

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Abbas Ali ◽  
Nurhayat Tabanca ◽  
Betul Demirci ◽  
K. Husnu Can Baser ◽  
Jane Ellis ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Plant Pathogens ◽  
Gas Chromatography Mass Spectrometry ◽  
Moderate Activity ◽  
Female Cone ◽  
Fungal Plant Pathogens ◽  
Cupressus Arizonica

Essential oils obtained by hydrodistillation of female cones (FC), male cones (MC), needle-twigs (NT) and wood-bark (WB) of ‘Carolina Sapphire’ [Cupressus arizonica var glabra (Sudw.) Little] were analyzed simultaneously by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The main constituents of the investigated essential oils were as follows: FC: α-pinene 68.5% and myrcene 11.9%; MC: α-pinene 22.5%, epi- zonarene 9.9%, zonarene 6.9%, limonene 6.8% and cadina-3,5-diene 6.1%; NT: α-pinene 20.7%, epi-zonarene 10.4%, zonarene 7.6%, limonene 5.9% umbellulone 5.8% and cadina-3,5-diene 5.8%; WB: α-pinene 40.7%, limonene 3.2% and umbellulone 2.9%. Essential oils were evaluated for antifungal activity against the strawberry anthracnose causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. Only WB essential oil showed moderate activity when compared with the commercial antifungal standards. Carolina Sapphire essential oils exhibited biting deterrent activity at 10 pg/cm2 with Biting Deterrence Index (BDI) values of 0.64, 0.59, 0.65 and 0.72, for FC, MC, NT and WB, respectively, and were significantly lower (P≤0.05) than the synthetic insect repellent, DEET (N,N-diethyl-meta-toluamide), at 25 nmol/cm2 against Aedes aegypti. The biting deterrence of NT and WB oils at 100 μg/cm2 with BDI values of 1.04 and 1.01, respectively, were similar, whereas the activity of MC (BDI=0.88) and FC (BDI=0.62) essential oils was lower than DEET at 25 nmol/cm2. Essential oil from FC with LD50 of 33.7 ppm was most toxic against 1-day-old Ae. aegypti larvae at 24-h post treatment. Female cone essential oil was significantly more toxic than MC (LD50=53.6 ppm), NT (LD50=55.5 ppm) and WB (LD50=44.6 ppm) essential oils.

Douglas-fir root-associated microorganisms with inhibitory activity towards fungal plant pathogens and human bacterial pathogens

1996 ◽  
Vol 42 (7) ◽  
pp. 690-700 ◽  
Author(s):  
Paige E. Axelrood ◽  
Alison M. Clarke ◽  
Reed Radley ◽  
S. Janet V. Zemcov
Keyword(s):  
Antifungal Activity ◽  
Inhibitory Activity ◽  
Plant Pathogens ◽  
Bacterial Pathogens ◽  
Douglas Fir ◽  
Microbial Culture ◽  
Bacterial Strains ◽  
Fungal Plant Pathogens ◽  
Forest Sites

A microbial culture collection composed of 1820 bacterial strains, including 298 actinomycete strains, was established from the roots of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings harvested from conifer nurseries and forest sites. Two hundred and thirty-four strains inhibited the growth of Fusarium, Cylindrocarpon, and (or) Pythium spp. in in vitro assays. A significantly greater proportion of bacterial strains from actinomycete genera exhibited antifungal properties compared with bacterial strains from nonactinomycete genera. Eighty-nine percent of identified inhibitory strains were Streptomyces, Streptoverticillium, Bacillus, Pseudomonas, or Burkholderia species. The actinomycete species were isolated almost exclusively from forest seedlings. Recovery of inhibitory strains representing 29 microbial species was enhanced using a variety of methods to isolate microorganisms from the roots of seedlings from nursery and forest sites. Bacterial strains (including actinomycete strains) with antifungal activity were tested for in vitro growth inhibition of six clinical human bacterial pathogens (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa). Forty-eight percent of the tested strains inhibited one or more human pathogens. Inhibitory activity towards fungal and bacterial pathogens was strain specific, not species specific, and many inhibitory strains exhibited broad-spectrum activity. Strains with antifungal activity against several conifer root pathogens were also more likely to inhibit multiple species of clinical bacterial pathogens.Key words: in vitro, antimicrobial, conifer rhizosphere.

scholarly journals Étude Phytochimique et activité antifongique d'extraits de quelques Euphorbiaceae médicinales utilisées chez les Baoulé du District de Yamoussoukro (Côte d'Ivoire)

2018 ◽  
Vol 14 (30) ◽  
pp. 256
Author(s):  
Saraka Allou Isidore ◽  
Abo Kouabenan ◽  
Coulibaly Kiyinlma ◽  
Zirihi Guédé Noël
Keyword(s):  
Antifungal Activity ◽  
Cote D'ivoire ◽  
Plant Pathogens ◽  
Côte D’Ivoire ◽  
Aqueous Extracts ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Fungal Plant Pathogens ◽  
Ethanolic Extracts ◽  
Fusarium Sp

An ethnobotanical survey was carried out in the District of Yamoussoukro (Central Côte d’Ivoire) in order to evaluate the biocontrol potential against fungal plant pathogens, of Euphorbiaceous plants used as medicines by the native population. Twelve (12) species were collected. The antifungal activity was assessed using the two-fold dilution method on PDA medium with ethanolic and aqueous extract derived from leaves of six plants. Results pointed out that only 70% ethanolic extracts from Alchornea cordifolia (EE13) possess fungicidal properties on both Fusarium sp. and Phytophthora sp. The antifungal activities are more effective with ethanolic extracts than aqueous extracts. With a minimal inhibitory concentration MIC = 25±0.0 mg/ml, a minimal concentration for 50% of inhibition IC50 = 10±0.05 mg/ml and a minimal fungicide concentration MFC = 200±0.0 mg/ml Fusarium sp. appears to be the most sensitive. Phytophthora sp. is less sensitive with a MIC = 50±0.0 mg/ml, an IC50 = 12±0.05 mg/ml and a MFC = 200±0.0 mg/ml. Phytochemical screening revealed the presence of seven major groups of compounds among which saponins and catechin are the most abundant in aqueous extracts. Polyphenols, flavonoids, polyterpenes and sterols as well as tannins are present in both alcoholic and aqueous extracts of Alchornea cordifolia. These compounds could justify its antifungal activity and traditional use.

scholarly journals Bioassays Guided Fractionation of Senna occidentalis for Identification of Natural Antifungal Constituents Against Macrophomina phaseolina

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. JAVAID ◽  
H. QUDSIA ◽  
A. SHOAIB
Keyword(s):  
Antifungal Activity ◽  
Fungal Biomass ◽  
Leaf Extract ◽  
Plant Pathogens ◽  
Macrophomina Phaseolina ◽  
Initial Screening ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Fungal Plant Pathogens ◽  
Benzenedicarboxylic Acid

ABSTRACT Macrophomina phaseolina is one of the most destructive soil-borne fungal plant pathogens. In the present study, different aerial parts of Senna occidentalis were analyzed for their antifungal activity against M. phaseolina. In initial screening bioassays, 0.5-3.0% concentrations of methanolic extracts of fruit, leaf and stem were tested against M. phaseolina. The methanolic leaf extract showed the greatest activity causing up to 29% suppression in biomass production of M. phaseolina. Fractionation of leaf extract of S. occidentalis was carried out with four organic solvents. Bioassays with a range of concentrations (2.34-150 mg mL-1) of these sub-fractions revealed that the chloroform sub-fraction was the most effective, causing 93-98% reduction in the biomass of M. phaseolina, followed by 59-92% suppression in fungal biomass due to the n-hexane sub-fraction. The ethyl acetate and n-butanol sub-fractions were only effective at higher concentrations. GC-MS analysis of chloroform sub-fraction was performed to identify different compounds. Six compounds were identified in this fraction; 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester was the predominant compound (53.55%) followed by 9,10-dimethyltricyclo[4.2.1.1 (2,5)]decane-9,10-diol (22.68%), cyclohexanol, and 2-(2-hydroxy-2-propyl)-5-methyl (9.87%).

Synthesis and antifungal activity of 2-allylphenol derivatives against fungal plant pathogens

2017 ◽  
Vol 135 ◽  
pp. 47-51 ◽  
Author(s):  
Tianli Qu ◽  
Shumei Gao ◽  
Jianqiang Li ◽  
Jianjun J. Hao ◽  
Pingsheng Ji
Keyword(s):  
Antifungal Activity ◽  
Plant Pathogens ◽  
Fungal Plant Pathogens

Characterization of Volatile Constituents from Origanum onites and Their Antifungal and Antibacterial Activity

2013 ◽  
Vol 96 (6) ◽  
pp. 1200-1208 ◽  
Author(s):  
Ayhan Altintas ◽  
Nurhayat Tabanca ◽  
Erno Tyihák ◽  
Péter G Ott ◽  
Ágnes M Móricz ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Growth Inhibition ◽  
Essential Oils ◽  
Plant Pathogens ◽  
Colletotrichum Acutatum ◽  
Reaction Products ◽  
Colletotrichum Species ◽  
Fungal Plant Pathogens ◽  
Origanum Onites

Abstract Essential oils obtained by hydrodistillation (HD) and microwave-assisted HD (MWHD) of Origanum onites aerial parts were analyzed by GC and GC/MS. Thirty-one constituents representing 98.6% of the water-distilled oil and 52 constituents representing 99.6% of the microwave-distilled oil were identified. Carvacrol (76.8% HD and 79.2% MWHD) and thymol (4.7% HD and 4.4% MWHD) were characterized as major constituents in both essential oils. Separation of carvacrol and thymol was achieved by overpressured layer chromatography. HPTLC and TLC separations were also compared. Essential oils were evaluated for antifungal activity against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides using a direct overlay bioautography assay. Furthermore, main oil components carvacrol and thymol were then evaluated for antifungal activity; only carvacrol demonstrated nonselective antifungal activity against the three Colletotrichum species. Thymol and carvacrol were subsequently evaluated in a 96-well microdilution broth assay against Phomopsis obscurans, Fusarium oxysporum, three Colletotrichum species, and Botrytis cinerea. No activity was observed against any of the three Colletotrichum species at or below 30 μM. However, thymol demonstrated antifungal activity and produced 31.7% growth inhibition of P. obscurans at 120 h and 0.3 μM, whereas carvacrol appeared inactive. Thymol and carvacrol at 30 μM showed 51.5 and 36.9% growth inhibition of B. cinerea at 72 h. The mechanism of antibacterial activity was studied in a bioautography-based BioArena system. Thymol and carvacrol showed similar inhibition/killing effect against Bacillus subtilis soil bacteria; the action could be enhanced by the formaldehyde generator and transporter copper(II) ions and could be decreased in the presence of L-arginine, a formaldehyde capturer. Results indicated that Origanum essential oils and its major components thymol and carvacrol appear to generate antimicrobial activity through a mechanism of action where formaldehyde and its reaction products are produced.

scholarly journals Essential Oil Composition of Five Collections of Achillea Biebersteinii from Central Turkey and their Antifungal and Insecticidal Activity

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Nurhayat Tabanca ◽  
Betül Demirci ◽  
İlhan Gürbüz ◽  
Fatih Demirci ◽  
James J. Becnel ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Plant Pathogens ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Fungal Plant Pathogens ◽  
Two Samples ◽  
Achillea Biebersteinii ◽  
Central Turkey

The composition of the essential oils hydrodistilled from the aerial parts of five Achillea biebersteinii Afan samples, collected in central Turkey from Konya, Isparta and Ankara, were analyzed both by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Eighty-four components were identified, representing 87 to 99% of the total oil composition. The identified major components were 1,8-cineole (9-37%), camphor (16-30%) and p-cymene (1-27%). Two samples differed in piperitone (11%) and ascaridol (4%) content. The five A. biebersteinii essential oils were subsequently evaluated for their antifungal activity against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using the direct overlay bioautography assay. The essential oils showed no antifungal activity at 80 and 160 μg/spot. In addition, A. biebersteinii oils and their major compounds were subsequently investigated against Aedes aegypti first instar larvae in a high throughput bioassay. Among the oils, only one sample from Ankara showed a notable larvacidal effect on Ae. aegypti larvae. The major compounds, 1,8-cineole, camphor and p-cymene, exhibited low mosquito larval activity, and thus the minor compounds are probably responsible for the observed activity against Ae. aegypti larvae. The oils showed weak activity against adult Ae. aegypti.

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