Effects of Seed Storage on Germination of Desert Halophytes with Transient Seed Bank

Author(s):  
Ali El-Keblawy
Keyword(s):  
2021 ◽  
Vol 27 (3) ◽  
pp. 162-166
Author(s):  
Abdul Azeez Hussain ◽  
◽  
Ramachandra Kurup Rajvikraman ◽  

Detailed study on seed storage and germination trailed in Knema attenuata (Wall. ex Hook. f. & Thomson) Warb.– the IUCN Red Listed ‘least concern’ medicinal tree species revealed that seeds were of recalcitrant nature. Viability of the seeds could be maintained for a longer period of up to 6 months with 47% Moisture content (mc) when kept in closed polycarbonate bottles at seed bank condition [20±20C Temp. and 40% Relative Humidity (RH)]. The 55% seed germination under normal climatic condition could be enhanced to a much higher percentage (75±5) inside the mist house chamber (34±30C Temp. and 70-80% RH).


2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.


2007 ◽  
Vol 55 (1) ◽  
pp. 18 ◽  
Author(s):  
Andrew D. Crawford ◽  
Kathryn J. Steadman ◽  
Julie A. Plummer ◽  
Anne Cochrane ◽  
Robin J. Probert

The suitability of applying international standards for long-term seed storage to the Australian flora was examined by reviewing seed-storage data from the Western Australian Threatened Flora Seed Centre. The 375 collections examined represented 176 taxa from 44 genera and 16 families. A high proportion of collections, representative of some of the most common genera in Australia, maintained viability in the short (<5 years) and medium (5–12 years) term. Declines in germination were evident for a small number of collections, representing 10 taxa, stored for 5–12 years. However, many of the declines were collection-specific and other collections of the same taxon did not decline. Five taxa showing germination declines were represented by a single collection so it was not possible to determine whether the germination decline was directly related to the taxa, storage conditions or to other factors. Only the closely related Stylidium coroniforme and S. amabile exhibited germination declines in all collections, but cut tests of seeds remaining from germination testing indicated that viability of the collections had not declined, just the proportion to germinate; for these species a change in germination conditions is a more likely explanation for the reduction in germination. The results illustrate the successful application of these seed-storage standards to threatened flora in Western Australia and highlight their suitability for the Australian flora.


1991 ◽  
Vol 39 (4) ◽  
pp. 385 ◽  
Author(s):  
ETF Witkowski ◽  
BB Lamont ◽  
SJ Connell

Seed bank dynamics of three co-occurring, non-sprouting Banksia species (B. baxteri, B. speciosa and B. coccinea) in patch-burnt scrub-heath (aged 10 and 21 years) were studied in the southern sandplains of Western Australia. In the younger plants, canopy seed storage was highest in B. coccinea. However, 21-year-old B. coccinea stored an order of magnitude fewer seeds than the other species and no more than young B. coccinea. Seed storage per year increased exponentially with plant age in B. speciosa and B. baxteri, whereas a quadratic function, peaking at 16 years, best described seed storage in B. Coccinea. Degree of serotiny was lowest in B. coccinea. Seed abortion did not vary between age cohorts but was highest in B. coccinea. Granivory ranged from 18 to 42% and was highest in the older plants and in B. baxteri. Cockatoos removed about 20% of cones in B. baxteri and B. coccinea and 10% in B. speciosa. Experimental cone removal accelerated follicle opening, especially in B. coccinea, irrespective of whether cones were placed on the ground (simulating cockatoo removal) or reattached to the plant (simulating plant death). Half the 21-year-old B. coccinea were dead and the remainder were considered senescent, as most branches showed dieback and cone fertility had fallen to 8%. Death of the other species was negligible, with cone fertility rising to a mean of 45% (B. speciosa) and 66% (B. baxteri). Interfire establishment was significant only in B. coccinea. The build-up of a viable seed bank with time occurred at a slower rate than for non-sprouting banksias in the northern sandplains.


2024 ◽  
Vol 84 ◽  
Author(s):  
A. Ahmad ◽  
A. Saeed ◽  
A. B. Gulshan ◽  
S. Wali ◽  
F. Hadi ◽  
...  

Abstract The current focus is on the overall pattern of seed storage present in the fragments of the soil of piedmont and alluvial landscapes of the environment. The present study predicted the seed banks of both soils of alluvial and piedmont zones in different ecological conditions and evaluate the potential of seeds in the restoration of both environments. The composition of the seed bank of soil is mainly affected by the alluvial environment and the structure of cleared area shows that more species of germinating annual grasses and growable seeds with the higher total number. Extant vegetation structures have an important role in the diversity of soil seed reservoirs, whose composition corresponded with the openness of the site. When in situ soil seed bank is recruited, it helps to restore only some components of the plant community in an alluvial environment. In our current research, it was confirmed that seed richness was higher in number at lower elevation (alluvial) than that at high elevation (piedmont). Seed richness showed a significant negative correlation with anions, cations, while significantly positive with altitude that suggests the richness pattern of the overall seed bank of the area is influenced by various environmental factors.


2020 ◽  
Vol 30 (2) ◽  
pp. 101-111
Author(s):  
Rachael M. Davies ◽  
Alice R. Hudson ◽  
John B. Dickie ◽  
Charlotte Cook ◽  
Tom O'Hara ◽  
...  

AbstractUK trees require increased conservation efforts due to sparse and fragmented populations. Ex situ conservation, including seed banking, can be used to better manage these issues. We conducted accelerated ageing tests on seeds of 22 UK native woody species, in order to assess their likely longevity and optimize their conservation in a seed bank. Germination at four ageing time points was determined to construct survival curves, and it was concluded that multiple samples within a species showed comparable responses for most species tested, except for Fraxinus excelsior. Of all species studied, one could be classified as very short-lived, four as short-lived and 17 as medium, with none exceeding the medium category. The most important finding of this manuscript is that although some taxonomic trends were observed, the results indicate the need for caution when making broad conclusions on potential seed storage life at a species, genus or family level. Longevity predictions were compared to actual performance of older collections held in long-term storage at the Millennium Seed Bank, Kew. Although most collections remain high in viability in storage after more than 20 years, for short-lived species at least, there is some indication that accelerated ageing predicts longevity in seed bank conditions. For species with reduced potential longevity, such as Fagus sylvatica and Ulmus glabra, additional storage options are recommended for long-term gene banking.


2019 ◽  
Vol 2 (1) ◽  
pp. 14-35
Author(s):  
Bal Krishna Joshi ◽  
Deepak Upadhya

Agricultural biodiversity is the basis for agricultural research and development. This paper is based on the survey across the country along with the field implementation and action research on different conservation approaches over the years and locations. Management of agricultural biodiversity is being initiated in Nepal through ex-situ, on-farm, in-situ and breeding strategies by National Agriculture Genetic Resources Center (National Genebank) from 2010. National Genebank has implemented on-farm conservation across the country. Different approaches are community seed bank, community field genebank, household seed bank, household field genebank, village level field genebank and school field genebank. In addition to these, landrace enhancement program and recognition of agro-biodiversity rich farmers have also contributed on conserving APGRs. Identification of agro-biodiversity rich farmers have been initiated in collaboration with different governmental and non-governmental organizations across the country. Strong network among these farmers is supposed to establish for effectively managing APGRs on-farm. Agro-biodiversity conserved by such farmers is called household genebank, which consist of seed storage (household seed bank) and household field genebank. There are more than 100 CSBs in the country and some of them are conserving local genetic resources. Eight community field genebanks have been functionalized for conserving recalcitrant seeds and vegetatively propagated crop species. Village level field genebank has been established by NGO in Makwanpur district to conserve banana and taro. In this approach, different households grow different landraces, maintaining maximum diversity within a village. In some areas, school field genebank has been established by growing local APGRs in school areas. This helps to conserve APGRs, educate the students, earn the income and make environment beautiful. Genebank also encourages farmers to reintroduce the landraces from the National Genebank. Accessioning these APGRs and training to farmers and farming communities are necessary to effectively and efficiently conserving and utilizing APGRs on-farm.


2007 ◽  
Vol 17 (4) ◽  
pp. 233-242 ◽  
Author(s):  
Christina Walters

AbstractEfficient seed storage is a shared concern among the growing number of seed banks established for crop improvement or ex situ conservation. Container properties greatly affect seed interactions with the environment and the overall cost and success of seed banking operations. Several material properties contribute to their suitability as seed containers. This paper provides a consolidated list of water vapour permeability properties of thermal plastics commonly used for packaging. Composite packages with layers of film with different properties provide distinct advantages to seed banks. Different seed banks must rank the importance of the various factors depending on their mission and resources. Once the risks, costs and benefits are weighed, an appropriate strategy can be developed that addresses a seed bank's specific needs. Because there are many problems and several solutions, it is likely that strategies will vary among seed banks. This response details variables to consider when selecting seed storage packages, and focuses on water diffusion rates of packages with different compositions. A ‘moisture audit’ will help seed bank operators make informed decisions about packaging.


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