Carbonic Anhydrases: The Mechanistic, Spectroscopic, and Model Studies

1990 ◽  
pp. 145-180
Author(s):  
R. S. Brown
Keyword(s):  
Carbonic Anhydrases ◽  
Model Studies

“In vitro” and in Animal Model Studies on a Double Virus- Inactivated Factor VIII Concentrate

1995 ◽  
Vol 74 (03) ◽  
pp. 868-873 ◽  
Author(s):  
Silvana Arrighi ◽  
Roberta Rossi ◽  
Maria Giuseppina Borri ◽  
Vladimir Lesnikov ◽  
Marina Lesnikov ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Animal Model ◽  
Factor Viii ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Inactivation ◽  
Enveloped Viruses ◽  
Model Studies ◽  
Heat Treated

SummaryTo improve the safety of plasma derived factor VIII (FVIII) concentrate, we introduced a final super heat treatment (100° C for 30 min) as additional virus inactivation step applied to a lyophilized, highly purified FVIII concentrate (100 IU/mg of proteins) already virus inactivated using the solvent/detergent (SID) method during the manufacturing process.The efficiency of the super heat treatment was demonstrated in inactivating two non-lipid enveloped viruses (Hepatitis A virus and Poliovirus 1). The loss of FVIII procoagulant activity during the super heat treatment was of about 15%, estimated both by clotting and chromogenic assays. No substantial changes were observed in physical, biochemical and immunological characteristics of the heat treated FVIII concentrate in comparison with those of the FVIII before heat treatment.

Melatonin modifies tumor hypoxia and metabolism by inhibiting HIF-1α and energy metabolic pathway in the in vitro and in vivo models of breast cancer

2019 ◽  
Vol 2 (4) ◽  
pp. 83-98 ◽  
Author(s):  
André De Lima Mota ◽  
Bruna Vitorasso Jardim-Perassi ◽  
Tialfi Bergamin De Castro ◽  
Jucimara Colombo ◽  
Nathália Martins Sonehara ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Glucose Metabolism ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Carbonic Anhydrases ◽  
In Vivo Models ◽  
Ca Ix ◽  
Gene And Protein Expression

Breast cancer is the most common cancer among women and has a high mortality rate. Adverse conditions in the tumor microenvironment, such as hypoxia and acidosis, may exert selective pressure on the tumor, selecting subpopulations of tumor cells with advantages for survival in this environment. In this context, therapeutic agents that can modify these conditions, and consequently the intratumoral heterogeneity need to be explored. Melatonin, in addition to its physiological effects, exhibits important anti-tumor actions which may associate with modification of hypoxia and Warburg effect. In this study, we have evaluated the action of melatonin on tumor growth and tumor metabolism by different markers of hypoxia and glucose metabolism (HIF-1α, glucose transporters GLUT1 and GLUT3 and carbonic anhydrases CA-IX and CA-XII) in triple negative breast cancer model. In an in vitro study, gene and protein expressions of these markers were evaluated by quantitative real-time PCR and immunocytochemistry, respectively. The effects of melatonin were also tested in a MDA-MB-231 xenograft animal model. Results showed that melatonin treatment reduced the viability of MDA-MB-231 cells and tumor growth in Balb/c nude mice (p <0.05). The treatment significantly decreased HIF-1α gene and protein expression concomitantly with the expression of GLUT1, GLUT3, CA-IX and CA-XII (p <0.05). These results strongly suggest that melatonin down-regulates HIF-1α expression and regulates glucose metabolism in breast tumor cells, therefore, controlling hypoxia and tumor progression. 

The Nature of Mining-Induced Horizontal Displacement of Surface on the Example of Several Coal Mines

2014 ◽  
Vol 59 (4) ◽  
pp. 971-986 ◽  
Author(s):  
Krzysztof Tajduś
Keyword(s):  
Underground Mining ◽  
Mining Industry ◽  
Horizontal Displacement ◽  
Surface Slope ◽  
Proportionality Coefficient ◽  
Model Studies ◽  
Displacement Vectors ◽  
Mining Conditions ◽  
Design Projects ◽  
Academy Of Sciences

Abstract The paper presents the analysis of the phenomenon of horizontal displacement of surface induced by underground mining exploitation. In the initial part, the basic theories describing horizontal displacement are discussed, followed by three illustrative examples of underground exploitation in varied mining conditions. It is argued that center of gravity (COG) method presented in the paper, hypothesis of Awierszyn and model studies carried out in Strata Mechanics Research Institute of the Polish Academy of Sciences indicate the proportionality between vectors of horizontal displacement and the vector of surface slope. The differences practically relate to the value of proportionality coefficient B, whose estimated values in currently realized design projects for mining industry range between 0.23r to 0.42r for deep exploitations, whereas in the present article the values of 0.33r and 0.47r were obtained for two instances of shallow exploitation. Furthermore, observations on changes of horizontal displacement vectors with face advancement indicated the possibility of existence of COG zones above the mined-out field, which proved the conclusions of hitherto carried out research studies (Tajduś 2013).

Environmental model studies for the Istanbul master plan. Part I: hydrodynamical design basis and marine disposal of wastewater

1995 ◽  
Vol 32 (2) ◽  
pp. 141-148 ◽  
Author(s):  
I. S. Hansen ◽  
H. J. Vested ◽  
M. A. Latif
Keyword(s):  
Black Sea ◽  
Layer Structure ◽  
The Black Sea ◽  
Marmara Sea ◽  
Junction Area ◽  
3 Dimensional ◽  
Model Studies ◽  
Model Set ◽  
Characteristic Features ◽  
Set Up

A modelling study of the hydrodynamics and spreading of wastewater from existing and future outfalls in the Bosphorus region has been conducted applying a 3-Dimensional model. The modelling is based on SYSTEM 3, which is a general modelling system for baroclinic flow simulating unsteady currents, waterlevels, salinity and temperature within the model area. The model set-up covers the Black Sea-Bosphorus-Marmara Sea junction area. The set-up is calibrated by data from a dedicated field program and previous field experience. The model is designed to describe the characteristic features of the flow in the junction area such as the effects of variations in waterlevel differences between the Sea of Marmara and the Black Sea on the important two-layer structure in the strait and the flow fields generated by the upper layer jet in the Bosphorus-Marmara junction. This model has been applied for evaluation of disposal of wastewater and for the subsequent water quality studies. The general use of a baroclinic 3-D hydrodynamic model to simulate disposal of wastewater is discussed. Examples of the application of the model of the junction area to evaluate the different strategies for disposal of wastewater are presented.

Sign in / Sign up

Export Citation Format

Share Document