Modelling Transmission of Pea Bacterial Blight (Pseudomonas syringae pv pisi) from Seed to Seedling

1997 ◽  
pp. 16-21
Author(s):  
Steven J. Roberts
Keyword(s):  
Pseudomonas Syringae ◽  
Bacterial Blight

scholarly journals Holdover Inoculum of Pseudomonas syringae pv. alisalensis from Broccoli Raab Causes Disease in Subsequent Plantings

Plant Disease ◽  
2006 ◽  
Vol 90 (8) ◽  
pp. 1077-1084 ◽  
Author(s):  
N. A. Cintas ◽  
S. T. Koike ◽  
R. A. Bunch ◽  
C. T. Bull
Keyword(s):  
Pseudomonas Syringae ◽  
Brassica Rapa ◽  
Bacterial Blight ◽  
Laboratory Tests ◽  
Bacterial Population ◽  
Disease Incidence ◽  
Untreated Soil ◽  
Bacterial Populations ◽  
Field Soils ◽  
Over Time

Uniform plots of broccoli raab (Brassica rapa subsp. rapa) seedlings were inoculated with a rifampicin-resistant strain of Pseudomonas syringae pv. alisalensis, the causal agent of bacterial blight on crucifers, resulting in 100% disease incidence in mature plants. Diseased plants were incorporated into the soil at maturity and smaller replicated plots were replanted at various times after incorporation. Rifampicin-resistant fluorescent pseudomonads with rep-PCR profiles identical to P. syringae pv. alisalensis were isolated from lesions on plants grown in soil into which the first diseased crop was incorporated. Disease incidence declined in mature plants as the length of time between incorporation of the first planting and seeding of the replanted plots increased. Bacterial population levels in soil decreased over time and bacteria were no longer detectable 3 weeks after incorporation of the diseased crop. In laboratory tests, population levels of P. syringae pv. alisalensis decreased in untreated soil but not in autoclaved soil. Greenhouse studies demonstrated a direct correlation between population levels of P. syringae pv. alisalensis applied to soil and disease incidence in seedlings. However, the decline in bacterial populations in field soils did not wholly account for the decline in disease incidence with subsequent plantings.

scholarly journals Green propolis ethanolic extract in bean plant protection against bacterial diseases

2019 ◽  
Vol 49 (6) ◽  
Author(s):  
Jonas Marcelo Jaski ◽  
Fabio Junior Telaxka ◽  
Gabriela Silva Moura ◽  
Gilmar Franzener
Keyword(s):  
Antimicrobial Activity ◽  
Pseudomonas Syringae ◽  
Bacterial Blight ◽  
Phenylalanine Ammonia Lyase ◽  
Plant Protection ◽  
Ethanolic Extract ◽  
Systemic Effect ◽  
Bacterial Diseases ◽  
Bean Plants ◽  
Maximum Activation

ABSTRACT: The aim of this study was to evaluate the ethanolic extract of green propolis (EEP) in the protection of common bean plants against two main bacterial cultures, bacterial blight (Xanthomonas axonopodis pv. phaseoli) and wildfire (Pseudomonas syringae pv. tabaci). Experiments on antimicrobial activity were performed, inducing phytoalexins, defense-related enzymes, and disease severity, under concentrations of 0, 0.5, 1.0, 2.5, and 5.0%. The EEP presented antimicrobial activity on both phytobacteria, causing a decrease in their development. It has also promoted a linear accumulation of phaseolin in bean hypocotyls according to the EEP concentration used. There was a reduction in the lesion area, which was caused by bacterial blight on bean leaves treated with EEP, and local and systemic effect were observed. Polyphenoloxidase was activated with 5% EEP, reaching the maximum activation time 62.5 h after application. An increase was observed in the activity of phenylalanine ammonia-lyase in plants treated with EEP, with local and systemic effect. Results indicated the potential of EEP in the control of these diseases.

Survival of Pseudomonas syringae pv. pisi in soil and on pea trash and their importance as a source of inoculum for a following field pea crop

1997 ◽  
Vol 37 (3) ◽  
pp. 369 ◽  
Author(s):  
G. J. Hollaway ◽  
T. W. Bretag
Keyword(s):  
Grain Yield ◽  
Pseudomonas Syringae ◽  
Field Trial ◽  
Bacterial Blight ◽  
Soil Surface ◽  
Field Studies ◽  
Field Pea ◽  
Crop Rotations ◽  
Controlled Environment ◽  
Field Peas

Summary. The importance of soil and field pea trash as sources of Pseudomonas syringae pv. pisi for infection of field pea was investigated both in a controlled environment and in the field. Studies of the survival of P. syringae pv. pisi in soil using autoclaved and non-autoclaved soil found that P. syringae pv. pisi is unlikely to survive in soil from one season to the next suggesting that soil is an unlikely source of inoculum in the field. However, Pseudomonas syringae pv. pisiwas able to survive on buried pea trash for at least 29 weeks and on pea trash positioned on the soil surface for at least 78 weeks. In a field trial, the presence of pea trash naturally infected with P. syringae pv. pisi caused significant bacterial blight and reduced grain yield of a field pea crop by 25%. Therefore, pea trash is a potent source of inoculum and crop rotations which include 2 seasons free of field peas should be considered as part of a strategy to control bacterial blight.

scholarly journals Development of an Engineered Bioluminescent Reporter Phage for Detection of Bacterial Blight of Crucifers

2012 ◽  
Vol 78 (10) ◽  
pp. 3592-3598 ◽  
Author(s):  
David A. Schofield ◽  
Carolee T. Bull ◽  
Isael Rubio ◽  
W. Patrick Wechter ◽  
Caroline Westwater ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Cultured Cells ◽  
Severe Disease ◽  
Disease Diagnosis ◽  
Diseased Plant ◽  
Content Type ◽  
Genes Encoding ◽  
Type Phage

ABSTRACTBacterial blight, caused by the phytopathogenPseudomonas cannabinapv.alisalensis, is an emerging disease afflicting important members of theBrassicaceaefamily. The disease is often misdiagnosed as pepper spot, a much less severe disease caused by the related pathogenPseudomonas syringaepv.maculicola. We have developed a phage-based diagnostic that can both identify and detect the causative agent of bacterial blight and differentiate the two pathogens. A recombinant “light”-tagged reporter phage was generated by integrating bacterialluxABgenes encoding luciferase into the genome ofP. cannabinapv.alisalensisphage PBSPCA1. The PBSPCA1::luxABreporter phage is viable and stable and retains properties similar to those of the wild-type phage. PBSPCA1::luxABrapidly and sensitively detectsP. cannabinapv.alisalensisby conferring a bioluminescent signal response to cultured cells. Detection is dependent on cell viability. Other bacterial pathogens ofBrassicaspecies such asP. syringaepv.maculicola,Pseudomonas marginalis,Pectobacterium carotovorum,Xanthomonas campestrispv.campestris, andX. campestrispv.raphanieither do not produce a response or produce significantly attenuated signals with the reporter phage. Importantly, the reporter phage detectsP. cannabinapv.alisalensison diseased plant specimens, indicating its potential for disease diagnosis.

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