Development of Regeneration Protocols to Exploit Somaclonal Variations in Lathyrus Sativus for Developing Toxin Free Cultivar

2003 ◽  
pp. 291-299
Author(s):  
S. L. Mehta ◽  
I. M. Santha
Keyword(s):  
Lathyrus Sativus ◽  
Somaclonal Variations

scholarly journals In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1297
Author(s):  
Chitralekha Shyam ◽  
Manoj Tripathi ◽  
Sushma Tiwari ◽  
Niraj Tripathi ◽  
Ravindra Solanki ◽  
...  
Keyword(s):  
Erucic Acid ◽  
Acid Content ◽  
Indian Mustard ◽  
Mother Plant ◽  
Erucic Acid Content ◽  
Oilseed Crop ◽  
In Vitro Production ◽  
Somaclonal Variations ◽  
Low Erucic Acid

Brassica junceais a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The plantlets regenerated from tissue cultures (R0), their R1 generation and respective parental lines were compared for morpho-physiological traits and fatty acid profile for the probable existence of somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% and 5.52% erucic acid in R0 and R1 regenerants, respectively, compared to the mother plant (41.36%). In comparison, the second somaclone acquired from PM30 exhibited a complete absence of erucic acid corresponding to its mother plant (1.07%). These putative somaclones present a source of variation for exploitation in the development of future mustard crops with low erucic acid content.

In situ effects of Lathyrus sativus- PGPR to remediate and restore quality and fertility of Pb and Cd polluted soils

2020 ◽  
Vol 192 ◽  
pp. 110260 ◽  
Author(s):  
Souhir Abdelkrim ◽  
Salwa Harzalli Jebara ◽  
Omar Saadani ◽  
Ghassen Abid ◽  
Wael Taamalli ◽  
...  
Keyword(s):  
Lathyrus Sativus ◽  
Polluted Soils

Tissue culture and genetic analysis of somaclonal variations of Solanum melongena L. cv. Nirrala

2014 ◽  
Vol 9 (12) ◽  
pp. 1182-1195
Author(s):  
Samar Naseer ◽  
Tariq Mahmood
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Soluble Protein ◽  
Solanum Melongena ◽  
Total Soluble Protein ◽  
Naphthalene Acetic Acid ◽  
Fresh Tissue ◽  
Somaclonal Variations ◽  
Random Amplification ◽  
Plant Grown

AbstractThe present study was designed to analyze genetically somaclonal variants using biochemical and molecular markers. Efficient tissue culture protocol for Solanum melongena L. cv. Nirrala was developed. Maximum callus induction (100%) was observed for Murashige and Skoog (MS) media supplemented with 2.0 mg L−1 naphthalene acetic acid +0.5 mg L−1 6-benzylaminopurine; and nodal explants gave best callusing response (88.8%) as compared to internodes (88.3%) and leaves (87.7%). The best shooting was induced on nodal and internodal callus in the presence of 2.0 mg L−1 6-benzylaminopurine. Total soluble protein content of callus and regenerated variant plants was estimated for biochemical analysis, and largest amount of soluble protein was found in callus (6.54 mg g−1 fresh tissue) followed by variant plant grown on 2.0 mg L−1 6-benzylaminopurine (5.96 mg g−1 fresh tissue). Random amplification of polymorphic DNA technique was done with five decamer primers (OPC1-OPC5) and maximum polymorphism was detected by OPC 2 (26.99%) among all samples, whereas nodal callus on media containing 1.0 mg L−1 naphthalene acetic acid +1.0 mg L−1 6-benzylaminopurine showed highest polymorphism producing 22 bands, out of which 8 bands were polymorphic. The study shows that this marker system can provide better evaluation of genetic variation induced by tissue culture.

EST-SSR analysis provides insights about genetic relatedness, population structure and gene flow in grass pea (Lathyrus sativus)

2015 ◽  
Vol 134 (3) ◽  
pp. 338-344 ◽  
Author(s):  
Khela R. Soren ◽  
Ashutosh Yadav ◽  
Gaurav Pandey ◽  
Priyanka Gangwar ◽  
Ashok K. Parihar ◽  
...  
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Genetic Relatedness ◽  
Lathyrus Sativus ◽  
Grass Pea ◽  
Ssr Analysis

Physicochemical values in different varieties of Lathyrus sativus and their interrelationships

1978 ◽  
Vol 26 (3) ◽  
pp. 687-689 ◽  
Author(s):  
Dwijendra Nath Roy ◽  
K. Visweswara Rao
Keyword(s):  
Lathyrus Sativus

Antioxidant properties of extracts from fermented and cooked seeds of Polish cultivars of Lathyrus sativus

2008 ◽  
Vol 109 (2) ◽  
pp. 285-292 ◽  
Author(s):  
Anna Starzyńska-Janiszewska ◽  
Bożena Stodolak ◽  
Małgorzata Jamróz
Keyword(s):  
Antioxidant Properties ◽  
Lathyrus Sativus

Physiological and Biochemical Analysis of Somaclones Derived from Leaf Explants of Lathyrus sativus

1995 ◽  
Vol 4 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Nirupama Tiwari ◽  
K. C. Bansal ◽  
K. All ◽  
Archana Sachdev ◽  
I. M. Santha ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Leaf Explants ◽  
Lathyrus Sativus ◽  
Physiological And Biochemical
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