Media optimization for maximum biomass production in cell cultures of pacific yew

1995 ◽  
Vol 42 (2) ◽  
pp. 185-193 ◽  
Author(s):  
R. E. B. Ketchum ◽  
D. M. Gibson ◽  
L. Greenspan Gallo
1978 ◽  
Vol 70 (6) ◽  
pp. 899-902 ◽  
Author(s):  
R. Kent Crookston ◽  
Carl A. Fox ◽  
David S. Hill ◽  
Dale N. Moss

Biomass ◽  
1988 ◽  
Vol 17 (4) ◽  
pp. 215-238 ◽  
Author(s):  
J.H. Cherney ◽  
K.D. Johnson ◽  
J.J. Volenec ◽  
K.S. Anliker

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Thibaud Taillefumier ◽  
Anna Posfai ◽  
Yigal Meir ◽  
Ned S Wingreen

Metagenomics has revealed hundreds of species in almost all microbiota. In a few well-studied cases, microbial communities have been observed to coordinate their metabolic fluxes. In principle, microbes can divide tasks to reap the benefits of specialization, as in human economies. However, the benefits and stability of an economy of microbial specialists are far from obvious. Here, we physically model the population dynamics of microbes that compete for steadily supplied resources. Importantly, we explicitly model the metabolic fluxes yielding cellular biomass production under the constraint of a limited enzyme budget. We find that population dynamics generally leads to the coexistence of different metabolic types. We establish that these microbial consortia act as cartels, whereby population dynamics pins down resource concentrations at values for which no other strategy can invade. Finally, we propose that at steady supply, cartels of competing strategies automatically yield maximum biomass, thereby achieving a collective optimum.


2021 ◽  
Author(s):  
Swati Sharan ◽  
Neera Bhalla Sarin ◽  
Kunal Mukhopadhyay

Abstract Ocimum tenuiflorum Linn. is an important aromatic medicinal plant which produces several secondary metabolites responsible for diverse pharmacological activities. The present study focuses on enhanced production of biomass as well as oleanolic acid (OA), an anticancer and antioxidant compound, in suspension cell cultures of O. tenuiflorum upon elicitation with different elicitors. Leaf explants derived friable calli were inoculated intol iquid Murashige and Skoog (MS) media containing plant growth regulators [0.25 mg/L of α-naphthaleneacetic acid (NAA) and 0.5 mg/L of 6-benzyl amino purine (BAP)] for the establishment of suspension cultures. Influence of several factors such as, age of the suspension cultures, different concentrations, and exposure times of various elicitors such as yeast extract (YE), methyl jasmonate (MeJ) and salicylic acid (SA) respectively were analysed for cell biomass production and accumulation of OA during this study. Among the elicitors tested, YE at 50 mg/L was found to be the most efficient in terms of increased biomass production and accumulation of OA in the cultures. The highest increase in OA production (13.16-fold in elicited cultures compared to untreated cultures) was noted on 17-day-old suspension cultures when exposed to 50 mg/L of YE for four days. Enhancement of 2.72-fold in OA content was also recorded in 17-day-old cultures when treated with MeJ (60 mg/L) during two days of exposure. SA was not efficient in inducing accumulation of OA in 17- and 22-day-old suspension cultures at any concentration and exposure time. Furthermore, it was observed that the effect of different elicitors on biomass production and OA content depended on concentration and the duration of exposure times. Therefore, utilization of elicitation method could be a promising tool to enhance cell growth and OA accumulation in the suspension cell cultures of O. tenuiflorum.


HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 603-606 ◽  
Author(s):  
Mark G. Lefsrud ◽  
Dean A. Kopsell ◽  
Robert M. Augé ◽  
A.J. Both

Consumption of fruit and vegetable crops rich in lutein and β-carotene carotenoids is associated with reduced risk of cancers and aging eye diseases. Kale (Brassica oleracea L. var. acephala D.C.) ranks highest for lutein concentrations and is an excellent source of dietary carotenoids. Kale plants were grown under varied photoperiods to determine changes in the accumulation of fresh and dry biomass, chlorophyll a and b, and lutein and β-carotene carotenoids. The plants were cultured in a controlled environment using nutrient solutions under photoperiod treatments of 6, 12, 16, or 24 hours (continuous). Fresh and dry mass production increased linearly as photoperiod increased, reaching a maximum under the 24-hour photoperiod. Maximum accumulation of lutein, β-carotene, and chlorophyll b occurred under the 24-h photoperiod at 13.5, 10.4, and 58.6 mg/100 g fresh mass, respectively. However, maximum chlorophyll a (235.1 mg/100 g fresh mass) occurred under the 12-hour photoperiod. When β-carotene and lutein were measured on a dry mass basis, the maximum accumulation was shifted to the 16-hour photoperiod. An increase in photoperiod resulted in increased pigment accumulation, but maximum concentrations of pigments were not correlated with maximum biomass production.


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