A novel synthetic vitamin D analogue, 2?-(3-hydroxypropoxy)1?, 25-dihydroxyvitamin D3 (ED-71), increases bone mass by stimulating the bone formation in normal and ovariectomized rats

The effects of two vitamin D3 metabolites, 24 R,25-dihydroxyvitamin D3 and lα,25-dihydroxyvitamin D3, were investigated in ovariectomized rats. The amount of ash in the femur on a defatted dry weight basis was significantly greater in rats treated with 1 μg/kg 24 R,25-dihydroxyvitamin D3, or 0.01 or 0.1 μg/kg lα,25-dihydroxyvitamin D3 than in the controls. The concentration of bone gla protein in serum and amounts in the femur were significantly greater in rats treated with 1 or 10 Mg/kg 24 R,25-dihydroxyvitamin D3, but not those given 1α,25-dihydroxyvitamin D3 compared with the controls. These results suggest that 24 R,25-dihydroxyvitamin D3 increased bone mass probably through the stimulation of bone formation.

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Effect of 1α,24(R)-dihydroxyvitamin D3 on calcium metabolism in the rat

Acta Endocrinologica ◽

10.1530/acta.0.1110572 ◽

1986 ◽

Vol 111 (4) ◽

pp. 572-576 ◽

Cited By ~ 5

Author(s):

S. Yamada ◽

J.-P. Bonjour ◽

H. Fleisch

Keyword(s):

Vitamin D ◽

Bone Resorption ◽

Bone Formation ◽

Urinary Excretion ◽

Intestinal Absorption ◽

Calcium Metabolism ◽

Significant Rise ◽

Whole Body ◽

Dihydroxyvitamin D3 ◽

Vitamin D Analogue

Abstract. It has been suggested that 1α,24(R)-dihy droxyvitamin D3 (1,24(OH)2D3), a chemically synthesized vitamin D analogue, may have the property to enhance whole-body Ca retention and may thus be of use in osteoporosis. In order to test this hypothesis the main fluxes of Ca metabolism were measured in vitamin D-replete rats injected ip with 1,24(OH)2D3 at daily doses of 25, 50 and 100 pmol for 10 days. As compared with pair fed control animals, rats treated with 1,24(OH)2D3 displayed a significant rise in net intestinal absorption of Ca and in urinary excretion of Ca, and increase in bone resorption but no significant change in bone formation. Whole body Ca retention was not changed at 25 pmol/day and showed a trend to decrease at 50 and 100 pmol/day. In conclusion these results do not suggest that among available vitamin D analogues, 1,24(OH)2D3 would be particularly useful for increasing bone Ca retention in osteoporosis.

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Evaluation of bone mineral density (BMD) and indicators of bone turnover in patients with hemophilia

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2018.2335 ◽

2018 ◽

Vol 18 (2) ◽

pp. 206-210 ◽

Cited By ~ 3

Author(s):

Mehmet Dagli ◽

Ali Kutlucan ◽

Sedat Abusoglu ◽

Abdulkadir Basturk ◽

Mehmet Sozen ◽

...

Keyword(s):

Bone Mineral Density ◽

Vitamin D ◽

Bone Formation ◽

Bone Mass ◽

Bone Mineral ◽

Type I ◽

Healthy Controls ◽

Mineral Density ◽

Lymphocyte Ratio ◽

Significant Difference

A decrease in bone mass is observed in hemophilic patients. The aim of this study was to evaluate bone mineral density (BMD), parathyroid hormone (PTH), 25-hydroxy vitamin D (vitamin D), and a bone formation and resorption marker, procollagen type I N-terminal propeptide (PINP) and urinary N-terminal telopeptide (uNTX) respectively, in hemophilic patients and healthy controls. Laboratory parameters related to the pathogenesis of bone loss such as neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) were also evaluated. Thirty-five men over 18 years of age, with severe hemophilia (A and B) and receiving secondary prophylaxis, were included in the study. The same number of age-, sex-, and ethnicity-matched healthy controls were evaluated. Anthropometric, biochemical, and hormonal parameters were determined in both groups. No significant difference in anthropometric parameters was found between the two groups. The BMD was low in 34% of hemophilic patients. Vitamin D, calcium, and free testosterone levels were significantly lower (p < 0.001, p = 0.011, p < 0.001, respectively), while PTH, PINP, and activated partial thromboplastin time (aPTT) levels were significantly higher (p < 0.014, p = 0.043, p < 0.001, respectively), in hemophilic patients compared to controls. There was no significant difference between the two groups in NLR, PLR, phosphorus, thyroid-stimulating hormone, and uNTX level. The reduction of bone mass in hemophilic patients may be evaluated using the markers of bone formation and resorption, enabling early detection and timely treatment.

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The Effect of Vitamin D and Its Metabolites on Fracture Repair in Chicks

Clinical Science ◽

10.1042/cs0650429 ◽

1983 ◽

Vol 65 (4) ◽

pp. 429-436 ◽

Cited By ~ 23

Author(s):

S. Dekel ◽

R. Salama ◽

S. Edelstein

Keyword(s):

Vitamin D ◽

Bone Formation ◽

Vitamin D3 ◽

Fracture Repair ◽

Control Group ◽

Clinical Implications ◽

Torsional Stress ◽

Active Vitamin ◽

Dihydroxyvitamin D3 ◽

24,25 Dihydroxyvitamin D3

1. One-day-old chicks were depleted of vitamin D. At 3 weeks their right tibiae, and those of a control group given vitamin D3, were fractured and pinned. After fracture the controls were kept on vitamin D3. Another group was left vitamin D-deficient. The remaining depleted chicks, divided into four groups, were given vitamin D3, 24,25-dihydroxyvitamin D3 [24,25(OH)2D3], 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] or a combination of 24,25(OH)2D3 and 1,25(OH)2D3. 2. The callus obtained after 9 and 14 days was subjected to torsional stress. The callus of chicks given vitamin D continuously showed the greatest resistance, whereas that of vitamin D-deficient chicks showed the smallest resistance. Repletion with either vitamin D3 or its metabolites increased the strength of the callus. Repletion with the combination of 24,25(OH)2D3 and 1,25(OH)2D3 produced the most marked results, in that the callus was even stronger than that of chicks replete with vitamin D3. 3. It is concluded that 24,25(OH)2D3 is essential for bone formation in addition to the known active vitamin D metabolite 1,25(OH)2D3, and the possible clinical implications of these findings are discussed.

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