Comparative transcription analysis of higher plant plastome mutants

1988 ◽  
Vol 14 (2) ◽  
pp. 171-176 ◽  
Author(s):  
K. Steiner ◽  
F. Baldauf ◽  
M. Metzlaff ◽  
R. Hagemann
Keyword(s):  
Higher Plant ◽  
Transcription Analysis ◽  
Plastome Mutants

Characterization of an iron-containing superoxide dismutase from a higher plant, Citrus limonum

1994 ◽  
Vol 90 (2) ◽  
pp. 339-347 ◽  
Author(s):  
Maria S. Almansa ◽  
Luis A. del Rio ◽  
Francisca Sevilla
Keyword(s):  
Superoxide Dismutase ◽  
Higher Plant ◽  
Citrus Limonum

Enhancing Yeast Transcription Analysis Through Integration of Heterogeneous Data

2004 ◽  
Vol 5 (8) ◽  
pp. 673-686 ◽  
Author(s):  
T. Grotkjaer ◽  
J. Nielsen
Keyword(s):  
Heterogeneous Data ◽  
Transcription Analysis

scholarly journals Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b-Deficient Rice (Oryza sativa L.)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Minh Khiem Nguyen ◽  
Tin-Han Shih ◽  
Szu-Hsien Lin ◽  
Jun-Wei Lin ◽  
Hoang Chinh Nguyen ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Core Protein ◽  
Profile Analysis ◽  
Oryza Sativa L ◽  
Electron Flow ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Transcription Analysis ◽  
Chl A ◽  
Chl Biosynthesis

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) and wild-type rice (Oryza sativa L.). Chl b-deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11. Two genes encoding PsbR (PSII core protein), FtsZ1, and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b-deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b. This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.

scholarly journals Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in Caenorhabditis elegans

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yisrael Rappaport ◽  
Hanna Achache ◽  
Roni Falk ◽  
Omer Murik ◽  
Oren Ram ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Rna Polymerase Ii ◽  
X Chromosome ◽  
Sex Chromosomes ◽  
Sex Chromosome ◽  
Transcription Analysis ◽  
X Chromosomes ◽  
Unique Character ◽  
Active Transcription ◽  
Heterogametic Sex

AbstractDuring meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and in heterogametic sex chromosomes. Initiation of sex chromosome silencing is disrupted in meiocytes with sex chromosome-autosome translocations. To determine whether this is due to aberrant synapsis or loss of continuity of sex chromosomes, we engineered Caenorhabditis elegans nematodes with non-translocated, bisected X chromosomes. In early meiocytes of mutant males and hermaphrodites, X segments are enriched with euchromatin assembly markers and active RNA polymerase II staining, indicating active transcription. Analysis of RNA-seq data showed that genes from the X chromosome are upregulated in gonads of mutant worms. Contrary to previous models, which predicted that any unsynapsed chromatin is silenced during meiosis, our data indicate that unsynapsed X segments are transcribed. Therefore, our results suggest that sex chromosome chromatin has a unique character that facilitates its meiotic expression when its continuity is lost, regardless of whether or not it is synapsed.

scholarly journals Molecular cloning and sequencing of cDNAs encoding the proteolipid subunit of the vacuolar H(+)-ATPase from a higher plant

1991 ◽  
Vol 266 (24) ◽  
pp. 16078-16084
Author(s):  
S.P. Lai ◽  
J.C. Watson ◽  
J.N. Hansen ◽  
H. Sze
Keyword(s):  
Molecular Cloning ◽  
Cloning And Sequencing ◽  
Higher Plant

scholarly journals Antisense Inhibition of the PsbX Protein Affects PSII Integrity in the Higher Plant Arabidopsis thaliana

2008 ◽  
Vol 50 (2) ◽  
pp. 191-202 ◽  
Author(s):  
José G. García-Cerdán ◽  
Dmitry Sveshnikov ◽  
David Dewez ◽  
Stefan Jansson ◽  
Christiane Funk ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Antisense Inhibition ◽  
Higher Plant ◽  
Plant Arabidopsis Thaliana

scholarly journals Comparative Metabolite Profile, Biological Activity and Overall Quality of Three Lettuce (Lactuca sativa L., Asteraceae) Cultivars in Response to Sulfur Nutrition

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 713
Author(s):  
Muna Ali Abdalla ◽  
Fengjie Li ◽  
Arlette Wenzel-Storjohann ◽  
Saad Sulieman ◽  
Deniz Tasdemir ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Sesquiterpene Lactones ◽  
Critical Role ◽  
Soluble Sugars ◽  
Radical Scavenging ◽  
Metabolite Profile ◽  
Water Soluble ◽  
Radical Scavenger ◽  
Higher Plant

The main objective of the present study was to assess the effects of sulfur (S) nutrition on plant growth, overall quality, secondary metabolites, and antibacterial and radical scavenging activities of hydroponically grown lettuce cultivars. Three lettuce cultivars, namely, Pazmanea RZ (green butterhead, V1), Hawking RZ (green multi-leaf lettuce, V2), and Barlach RZ (red multi-leaf, V3) were subjected to two S-treatments in the form of magnesium sulfate (+S) or magnesium chloride (−S). Significant differences were observed under −S treatments, especially among V1 and V2 lettuce cultivars. These responses were reflected in the yield, levels of macro- and micro-nutrients, water-soluble sugars, and free inorganic anions. In comparison with the green cultivars (V1 and V2), the red-V3 cultivar revealed a greater acclimation to S starvation, as evidenced by relative higher plant growth. In contrast, the green cultivars showed higher capabilities in production and superior quality attributes under +S condition. As for secondary metabolites, sixteen compounds (e.g., sesquiterpene lactones, caffeoyl derivatives, caffeic acid hexose, 5-caffeoylquinic acid (5-OCQA), quercetin and luteolin glucoside derivatives) were annotated in all three cultivars with the aid of HPLC-DAD-MS-based untargeted metabolomics. Sesquiterpene lactone lactucin and anthocyanin cyanidin 3-O-galactoside were only detected in V1 and V3 cultivars, respectively. Based on the analyses, the V3 cultivar was the most potent radical scavenger, while V1 and V2 cultivars exhibited antibacterial activity against Staphylococcus aureus in response to S provision. Our study emphasizes the critical role of S nutrition in plant growth, acclimation, and nutritional quality. The judicious-S application can be adopted as a promising antimicrobial prototype for medical applications.

scholarly journals Purification and initial characterization of ubiquitin from the higher plant, Avena sativa.

1985 ◽  
Vol 260 (22) ◽  
pp. 12015-12021 ◽  
Author(s):  
R D Vierstra ◽  
S M Langan ◽  
A L Haas
Keyword(s):  
Avena Sativa ◽  
Higher Plant ◽  
Initial Characterization
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