Characteristics of the antilysozyme activity ofStaphylococcus aureus in different types of experimental infection

1993 ◽  
Vol 115 (2) ◽  
pp. 178-181
Author(s):  
O. V. Bukharin ◽  
B. Ya. Usvyatsov ◽  
N. V. Sheenkov

Author(s):  
N. V. Morozova ◽  
◽  
М. V. Sycheva ◽  
V. I. Sorokin ◽  
◽  
...  

The research aim: to study the bioprophiles of microorganisms isolated from the urine of cats in the pathology of the urinary system (cystitis and urolithiasis). 46 strains of different types of bacteria were isolated from the urine of 34 sick animals by bacteriological method and identified using the biochemical test systems “Lachema” (“Erba Lachema s.r.o.”, European Union). The photometric method was used to determine the anti-lysozyme and anti-hemoglobin activity, the adhesion index, and the ability to form biofilms in isolates. It was established that microorganisms isolated from urolithiasis in cats were more often isolated in associations than microorganisms isolated from cystitis, among which monocultures prevailed. Biological properties of isolated microorganisms were studied, bioprofile were identified characterizing the strains. It is shown that cultures of S. aureus, E. coli, S. epidermidis from urine with urolithiasis was significantly higher values antigemoglobin activity; in E. coli – antilysozyme activity, in S. aureus – increased adhesion, in S. epidermidis – the ability to form biofilms than in urine strains with cystitis. P. aeruginosa strains isolated from urine with cystitis were characterized by a high level of adhesion compared to cultures of this species isolated from urine with urolithiasis. The results obtained can be used for differentiation of strains that can cause studied pathologies, as well as for selection of adequate therapy under the control of studied biological properties of microorganisms.



1996 ◽  
Vol 75 (11) ◽  
pp. 1365-1372 ◽  
Author(s):  
D. THIAGARAJAN ◽  
H.L. THACKER ◽  
A.M. SAEED


1986 ◽  
Vol 23 (04) ◽  
pp. 851-858 ◽  
Author(s):  
P. J. Brockwell

The Laplace transform of the extinction time is determined for a general birth and death process with arbitrary catastrophe rate and catastrophe size distribution. It is assumed only that the birth rates satisfyλ0= 0,λj> 0 for eachj> 0, and. Necessary and sufficient conditions for certain extinction of the population are derived. The results are applied to the linear birth and death process (λj=jλ, µj=jμ) with catastrophes of several different types.



2020 ◽  
Vol 43 ◽  
Author(s):  
Rajen A. Anderson ◽  
Benjamin C. Ruisch ◽  
David A. Pizarro

Abstract We argue that Tomasello's account overlooks important psychological distinctions between how humans judge different types of moral obligations, such as prescriptive obligations (i.e., what one should do) and proscriptive obligations (i.e., what one should not do). Specifically, evaluating these different types of obligations rests on different psychological inputs and has distinct downstream consequences for judgments of moral character.



Author(s):  
P.L. Moore

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.



Author(s):  
U. Aebi ◽  
P. Rew ◽  
T.-T. Sun

Various types of intermediate-sized (10-nm) filaments have been found and described in many different cell types during the past few years. Despite the differences in the chemical composition among the different types of filaments, they all yield common structural features: they are usually up to several microns long and have a diameter of 7 to 10 nm; there is evidence that they are made of several 2 to 3.5 nm wide protofilaments which are helically wound around each other; the secondary structure of the polypeptides constituting the filaments is rich in ∞-helix. However a detailed description of their structural organization is lacking to date.



Author(s):  
E. L. Thomas ◽  
S. L. Sass

In polyethylene single crystals pairs of black and white lines spaced 700-3,000Å apart, parallel to the [100] and [010] directions, have been identified as microsector boundaries. A microsector is formed when the plane of chain folding changes over a small distance within a polymer crystal. In order for the different types of folds to accommodate at the boundary between the 2 fold domains, a staggering along the chain direction and a rotation of the chains in the plane of the boundary occurs. The black-white contrast from a microsector boundary can be explained in terms of these chain rotations. We demonstrate that microsectors can terminate within the crystal and interpret the observed terminal strain contrast in terms of a screw dislocation dipole model.



Author(s):  
E.M. Kuhn ◽  
K.D. Marenus ◽  
M. Beer

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).



Author(s):  
G. D. Gagne ◽  
M. F. Miller ◽  
D. A. Peterson

Experimental infection of chimpanzees with non-A, non-B hepatitis (NANB) or with delta agent hepatitis results in the appearance of characteristic cytoplasmic alterations in the hepatocytes. These alterations include spongelike inclusions (Type I), attached convoluted membranes (Type II), tubular structures (Type III), and microtubular aggregates (Type IV) (Fig. 1). Type I, II and III structures are, by association, believed to be derived from endoplasmic reticulum and may be morphogenetically related. Type IV structures are generally observed free in the cytoplasm but sometimes in the vicinity of type III structures. It is not known whether these structures are somehow involved in the replication and/or assembly of the putative NANB virus or whether they are simply nonspecific responses to cellular injury. When treated with uranyl acetate, type I, II and III structures stain intensely as if they might contain nucleic acids. If these structures do correspond to intermediates in the replication of a virus, one might expect them to contain DNA or RNA and the present study was undertaken to explore this possibility.



Author(s):  
K. A. Brookes ◽  
D. Finbow ◽  
Madeleine Samuel

Investigation of the particulate matter contained in the water sample, revealed the presence of a number of different types and certain of these were selected for analysis.An A.E.I. Corinth electron microscope was modified to accept a Kevex Si (Li) detector. To allow for existing instruments to be readily modified, this was kept to a minimum. An additional port is machined in the specimen region to accept the detector, with the liquid nitrogen cooling dewar conveniently housed in the left hand cupboard adjacent to the microscope column. Since background radiation leads to loss in the sensitivity of the instrument, great care has been taken to reduce this effect by screening and manufacturing components that are near the specimen from material of low atomic number. To change from normal transmission imaging to X-ray analysis, the special 4-position specimen rod is inserted through the normal specimen airlock.



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