BIOPROFILE OF MICROORGANISMS ISOLATED FROM URINE OF CATS WITH DISEASES OF URINARY SYSTEM

The research aim: to study the bioprophiles of microorganisms isolated from the urine of cats in the pathology of the urinary system (cystitis and urolithiasis). 46 strains of different types of bacteria were isolated from the urine of 34 sick animals by bacteriological method and identified using the biochemical test systems “Lachema” (“Erba Lachema s.r.o.”, European Union). The photometric method was used to determine the anti-lysozyme and anti-hemoglobin activity, the adhesion index, and the ability to form biofilms in isolates. It was established that microorganisms isolated from urolithiasis in cats were more often isolated in associations than microorganisms isolated from cystitis, among which monocultures prevailed. Biological properties of isolated microorganisms were studied, bioprofile were identified characterizing the strains. It is shown that cultures of S. aureus, E. coli, S. epidermidis from urine with urolithiasis was significantly higher values antigemoglobin activity; in E. coli – antilysozyme activity, in S. aureus – increased adhesion, in S. epidermidis – the ability to form biofilms than in urine strains with cystitis. P. aeruginosa strains isolated from urine with cystitis were characterized by a high level of adhesion compared to cultures of this species isolated from urine with urolithiasis. The results obtained can be used for differentiation of strains that can cause studied pathologies, as well as for selection of adequate therapy under the control of studied biological properties of microorganisms.

Download Full-text

α-Amidoamids as New Replacements of Antibiotics—Research on the Chosen K12, R2–R4 E. coli Strains

Materials ◽

10.3390/ma13225169 ◽

2020 ◽

Vol 13 (22) ◽

pp. 5169

Author(s):

Paweł Kowalczyk ◽

Arleta Madej ◽

Mateusz Szymczak ◽

Ryszard Ostaszewski

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Biological Activity ◽

Plasmid Dna ◽

Alkyl Chain ◽

Antimicrobial Drugs ◽

E Coli ◽

Different Types ◽

Preliminary Study ◽

Selection Of

A preliminary study of α-amidoamids as new potential antimicrobial drugs was performed. Special emphasis was placed on selection of structure of α-amidoamids with the highest biological activity against different types of Gram-stained bacteria by lipopolysaccharide (LPS). Herein, Escherichia coli model strains K12 (without LPS in its structure) and R1–R4 (with different length LPS in its structure) were used. The presented work showed that the antibacterial activity of α-amidoamids depends on their structure and affects the LPS of bacteria. Moreover, the influence of various newly synthesized α-amidoamids on bacteria possessing smooth and rought LPS and oxidative damage of plasmid DNA caused by all newly obtained compounds was indicated. The presented studies clearly explain that α-amidoamids can be used as substitutes for antibiotics. The chemical and biological activity of the analysed α-amidoamids was associated with short alkyl chain and different isocyanides molecules in their structure such as: tetr-butyl isocyanide or 2,5-dimethoxybenzyl isocyanide. The observed results are especially important in the case of the increasing resistance of bacteria to various drugs and antibiotics.

Download Full-text

Electrospinning Nanofibers for Therapeutics Delivery

Nanomaterials ◽

10.3390/nano9040532 ◽

2019 ◽

Vol 9 (4) ◽

pp. 532 ◽

Cited By ~ 35

Author(s):

S. Shahriar ◽

Jagannath Mondal ◽

Mohammad Hasan ◽

Vishnu Revuri ◽

Dong Lee ◽

...

Keyword(s):

Drug Delivery ◽

Blood Circulation ◽

Biological Properties ◽

Detailed Mechanism ◽

Novel Therapeutics ◽

Therapeutic Drugs ◽

Different Types ◽

Materials Used ◽

Significant Attention ◽

Selection Of

The limitations of conventional therapeutic drugs necessitate the importance of developing novel therapeutics to treat diverse diseases. Conventional drugs have poor blood circulation time and are not stable or compatible with the biological system. Nanomaterials, with their exceptional structural properties, have gained significance as promising materials for the development of novel therapeutics. Nanofibers with unique physiochemical and biological properties have gained significant attention in the field of health care and biomedical research. The choice of a wide variety of materials for nanofiber fabrication, along with the release of therapeutic payload in sustained and controlled release patterns, make nanofibers an ideal material for drug delivery research. Electrospinning is the conventional method for fabricating nanofibers with different morphologies and is often used for the mass production of nanofibers. This review highlights the recent advancements in the use of nanofibers for the delivery of therapeutic drugs, nucleic acids and growth factors. A detailed mechanism for fabricating different types of nanofiber produced from electrospinning, and factors influencing nanofiber generation, are discussed. The insights from this review can provide a thorough understanding of the precise selection of materials used for fabricating nanofibers for specific therapeutic applications and also the importance of nanofibers for drug delivery applications.

Download Full-text

INTERMICROBIAL «SELF-NON-SELF» DISCRIMINATION IN «DOMINANT-ASSO-CIANT» PAIR OF PROBIOTIC STRAINS OF ESCHERICHIA COLI M-17 AND E.COLI LEGM-18

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-2016-3-3-9 ◽

2016 ◽

pp. 3-9

Author(s):

O. V. Bukharin ◽

N. B. Perunova ◽

E. V. Ivanova ◽

S. V. Andryuschenko

Keyword(s):

Escherichia Coli ◽

Pathogenicity Island ◽

Biological Properties ◽

Biological Characteristics ◽

Coliform Bacteria ◽

E Coli ◽

Growth Properties ◽

Probiotic Strains ◽

Antilysozyme Activity ◽

Special Growth

Aim. To use earlier developed method of intermicrobial «self-non-self» discrimination in «dominant-associant» pair for the assessment of foreignness of probiotic cultures of Escherichia coli M-17 (with pathogenicity island) and E. coli LEGM-18 (without pathogenicity island). Materials and methods. As dominants reference and clinical strains of bifidobacteria were used in the work, cultures of E. coli M-17 and E. coli LEGM-18 were taken as associants, differing in the presence of genes which code colibactin. Detection of the phenomenon of microbial discrimination was conducted according to the developed algorithm (Bukharin O.V., Perunova N.B., 2011) based on the principle of metabolite induction as a result of preliminary coincubation of dominants (bifidobacteria) with supernatant of associants and the formation of feed back in «dominant-as-sociant» pair. Special growth properties, biofilm formation, and antilysozyme activity served as biological characteristics of investigated coliform bacteria. Results. Testing of E. coli M -17 culture revealed depression of biological properties under investigation and it was estimated as «non-self» possibly due to the presence of pathogenicity island whereas E. coli LEGM-18 (without this fragment) sharply strengthened its biological characteristics and was subjected to assessment as «self». Conclusion. Use of intermicrobial «self-non-self» discrimination in «dominant-associant» pair is promising as basic method when selecting probiotic strains and cultures for creation of new symbiotic compositions and is suitable for quality control of probiotic products.

Download Full-text

Using Histological and Chemical Methods for Detection of Unauthorized Tissues Addition in Emulsion Type Meat Product

International Journal of Veterinary Science ◽

10.37422/ijvs/033 ◽

2020 ◽

Keyword(s):

Vascular Tissue ◽

Meat Product ◽

Calcium Content ◽

Processed Meat ◽

Low Protein ◽

Significant Difference ◽

Different Types ◽

Plant Stem ◽

High Level ◽

Reliable Methods

Total twenty different processed meat plant producing emulsion type sausage were histologically and chemically examined for detection of adulteration with unauthorized tissues. Results revealed that samples were adulterated with different types of animal tissues included; hyaline cartilage, tendon, spongy bone, peripheral nerve trunk, basophilic matrix, lymphatic tissue, fascia, fibrocartilage and vascular tissue. Moreover, these samples were adulterated Also, adulterated with plant tissue included; plant stem, leaves and root. Chemical analysis showed a significant difference in their chemical composition (moisture, fat, protein, ash and calcium) content. Moisture and fat content varied around the permissible limit of E.S.S. while low protein, high ash and calcium content was detected in the examined samples. Therefore, Histological and chemical examinations can be used as reliable methods to detect adultration using unauthorized addition of both animal and plant tissues in processed meat product samples which revealed a high level of falsification.

Download Full-text

Toxic Effect of 2-ethyl (bicyclo[2.2.1] heptane) on Bacterial Cells

Biotekhnologiya ◽

10.21519/0234-2758-2019-35-6-67-72 ◽

2019 ◽

Vol 35 (6) ◽

pp. 67-72 ◽

Cited By ~ 1

Author(s):

I.V. Manukhov ◽

L.S. Yaguzhinsky ◽

M.V. Bermeshev ◽

M.A. Zisman ◽

V.G. Pevgov ◽

...

Keyword(s):

Toxic Effect ◽

Atmospheric Oxygen ◽

Inducible Promoter ◽

Oxygen Species ◽

Bacterial Cells ◽

Unique Identifier ◽

E Coli ◽

Lux Biosensors ◽

High Level ◽

Ministry Of Higher Education

Toxic effect of 2-ethylnorbornane (2-ethyl(bicyclo[2.2.1]heptane) (EBH)) on bacteria has been studied using the E. coli pRecA-lux and E. coli pKatG- lux cells as lux-biosensors. It was shown that the addition of EBH to the incubation medium leads to death and growth retardation, high level oxidative stress and DNA damage in E. coli cells. It is assumed that the oxidation of EBH with atmospheric oxygen causes the formation of reactive oxygen species in the medium, which makes a major contribution to the toxicity of this substance. biosensor, luciferase, bioluminescence, inducible promoter, PrecA, PkatG The authors are grateful to Stanislav Filippovich Chalkin for the development of interdisciplinary ties in the scientific community. The work was financially supported by the Ministry of Higher Education and Science of Russia (Project Unique Identifier RFMEFI60417X0181, Agreement No. 14.604.21.0181 of 26.09.2017).

Download Full-text

NMR Assisted Antimicrobial Peptide Designing: Structure Based Modifications and Functional Correlation of a Designed Peptide VG16KRKP

Current Medicinal Chemistry ◽

10.2174/0929867326666190624090817 ◽

2020 ◽

Vol 27 (9) ◽

pp. 1387-1404 ◽

Cited By ~ 2

Author(s):

Karishma Biswas ◽

Humaira Ilyas ◽

Aritreyee Datta ◽

Anirban Bhunia

Keyword(s):

Antimicrobial Agents ◽

Biological Properties ◽

Structure Characterization ◽

Drug Resistant ◽

Functional Correlation ◽

Naturally Occurring ◽

High Resolution Nmr ◽

Different Types ◽

Reduced Activity ◽

Poor Stability

Antimicrobial Peptides (AMPs), within their realm incorporate a diverse group of structurally and functionally varied peptides, playing crucial roles in innate immunity. Over the last few decades, the field of AMP has seen a huge upsurge, mainly owing to the generation of the so-called drug resistant ‘superbugs’ as well as limitations associated with the existing antimicrobial agents. Due to their resilient biological properties, AMPs can very well form the sustainable alternative for nextgeneration therapeutic agents. Certain drawbacks associated with existing AMPs are, however, issues of major concern, circumventing which are imperative. These limitations mainly include proteolytic cleavage and hence poor stability inside the biological systems, reduced activity due to inadequate interaction with the microbial membrane, and ineffectiveness because of inappropriate delivery among others. In this context, the application of naturally occurring AMPs as an efficient prototype for generating various synthetic and designed counterparts has evolved as a new avenue in peptide-based therapy. Such designing approaches help to overcome the drawbacks of the parent AMPs while retaining the inherent activity. In this review, we summarize some of the basic NMR structure based approaches and techniques which aid in improving the activity of AMPs, using the example of a 16-residue dengue virus fusion protein derived peptide, VG16KRKP. Using first principle based designing technique and high resolution NMR-based structure characterization we validate different types of modifications of VG16KRKP, highlighting key motifs, which optimize its activity. The approaches and designing techniques presented can support our peers in their drug development work.

Download Full-text

Inorganic Gold and Polymeric Poly(Lactide-co-glycolide) Nanoparticles as Novel Strategies to Ameliorate the Biological Properties of Antimicrobial Peptides

Current Protein and Peptide Science ◽

10.2174/1389203720666191203101947 ◽

2020 ◽

Vol 21 (4) ◽

pp. 429-438 ◽

Cited By ~ 1

Author(s):

Bruno Casciaro ◽

Francesca Ghirga ◽

Deborah Quaglio ◽

Maria Luisa Mangoni

Keyword(s):

Antimicrobial Peptides ◽

Biological Properties ◽

Biological Profile ◽

Innovative Strategy ◽

Different Types ◽

New Antibiotics ◽

Interesting Class ◽

Alternative Antimicrobials ◽

Nanoparticulate Systems ◽

Infective Activity

Cationic antimicrobial peptides (AMPs) are an interesting class of gene-encoded molecules endowed with a broad-spectrum of anti-infective activity and immunomodulatory properties. They represent promising candidates for the development of new antibiotics, mainly due to their membraneperturbing mechanism of action that very rarely induces microbial resistance. However, bringing AMPs into the clinical field is hampered by some intrinsic limitations, encompassing low peptide bioavailability at the target site and high peptide susceptibility to proteolytic degradation. In this regard, nanotechnologies represent an innovative strategy to circumvent these issues. According to the literature, a large variety of nanoparticulate systems have been employed for drug-delivery, bioimaging, biosensors or nanoantibiotics. The possibility of conjugating different types of molecules, including AMPs, to these systems, allows the production of nanoformulations able to enhance the biological profile of the compound while reducing its cytotoxicity and prolonging its residence time. In this minireview, inorganic gold nanoparticles (NPs) and biodegradable polymeric NPs made of poly(lactide-coglycolide) are described with particular emphasis on examples of the conjugation of AMPs to them, to highlight the great potential of such nanoformulations as alternative antimicrobials.

Download Full-text

SBA MCQs for the MRCS Part A

10.1093/oso/9780199645633.001.0001 ◽

2012 ◽

Author(s):

Sri G. Thrumurthy ◽

Tania Samantha De Silva ◽

Zia Moinuddin ◽

Stuart Enoch

Keyword(s):

Clinical Case ◽

Multiple Choice ◽

Surgical Anatomy ◽

Surgical Patients ◽

Multiple Choice Questions ◽

Basic Sciences ◽

Self Assessment ◽

High Level ◽

Factual Recall ◽

Selection Of

Specifically designed to help candidates revise for the MRCS exam, this book features 350 Single Best Answer multiple choice questions, covering the whole syllabus. Containing everything candidates need to pass the MRCS Part A SBA section of the exam, it focuses intensively on the application of basic sciences (applied surgical anatomy, physiology, and pathology) to the management of surgical patients. The high level of detail included within the questions and their explanations allows effective self-assessment of knowledge and quick identification of key areas requiring further attention. Varying approaches to Single Best Answer multiple choice questions are used, giving effective exam practice and guidance through revision and exam technique. This includes clinical case questions, 'positively-worded' questions, requiring selection of the most appropriate of relatively correct answers; 'two-step' or 'double-jump' questions, requiring several cognitive steps to arrive at the correct answer; as well as 'factual recall' questions, prompting basic recall of facts.

Download Full-text

Multi-Criteria Selection of Additives in Porous Asphalt Mixtures Using Mechanical, Hydraulic, Economic, and Environmental Indicators

Sustainability ◽

10.3390/su13042146 ◽

2021 ◽

Vol 13 (4) ◽

pp. 2146

Author(s):

Anik Gupta ◽

Carlos J. Slebi-Acevedo ◽

Esther Lizasoain-Arteaga ◽

Jorge Rodriguez-Hernandez ◽

Daniel Castro-Fresno

Keyword(s):

Cellulose Fiber ◽

Environmental Indicators ◽

Asphalt Mixtures ◽

Environmental Benefits ◽

Modified Bitumen ◽

Porous Asphalt ◽

Different Types ◽

Product Assessment ◽

Polymer Modified Bitumen ◽

Selection Of

Porous asphalt (PA) mixtures are more environmentally friendly but have lower durability than dense-graded mixtures. Additives can be incorporated into PA mixtures to enhance their mechanical strength; however, they may compromise the hydraulic characteristics, increase the total cost of pavement, and negatively affect the environment. In this paper, PA mixtures were produced with 5 different types of additives including 4 fibers and 1 filler. Their performances were compared with the reference mixtures containing virgin bitumen and polymer-modified bitumen. The performance of all mixes was assessed using: mechanical, hydraulic, economic, and environmental indicators. Then, the Delphi method was applied to compute the relative weights for the parameters in multi-criteria decision-making methods. Evaluation based on distance from average solution (EDAS), technique for order of the preference by similarity to ideal solution (TOPSIS), and weighted aggregated sum product assessment (WASPAS) were employed to rank the additives. According to the results obtained, aramid pulp displayed comparable and, for some parameters such as abrasion resistance, even better performance than polymer-modified bitumen, whereas cellulose fiber demonstrated the best performance regarding sustainability, due to economic and environmental benefits.

Download Full-text

Photodynamic Therapy Combined with Antibiotics or Antifungals against Microorganisms That Cause Skin and Soft Tissue Infections: A Planktonic and Biofilm Approach to Overcome Resistances

Pharmaceuticals ◽

10.3390/ph14070603 ◽

2021 ◽

Vol 14 (7) ◽

pp. 603

Author(s):

Vanesa Pérez-Laguna ◽

Isabel García-Luque ◽

Sofía Ballesta ◽

Antonio Rezusta ◽

Yolanda Gilaberte

Keyword(s):

Photodynamic Therapy ◽

Soft Tissues ◽

Combined Treatment ◽

Resistance Pattern ◽

Antimicrobial Photodynamic Therapy ◽

Antimicrobial Drugs ◽

Bacteria And Fungi ◽

High Level ◽

Selection Of

The present review covers combination approaches of antimicrobial photodynamic therapy (aPDT) plus antibiotics or antifungals to attack bacteria and fungi in vitro (both planktonic and biofilm forms) focused on those microorganisms that cause infections in skin and soft tissues. The combination can prevent failure in the fight against these microorganisms: antimicrobial drugs can increase the susceptibility of microorganisms to aPDT and prevent the possibility of regrowth of those that were not inactivated during the irradiation; meanwhile, aPDT is effective regardless of the resistance pattern of the strain and their use does not contribute to the selection of antimicrobial resistance. Additive or synergistic antimicrobial effects in vitro are evaluated and the best combinations are presented. The use of combined treatment of aPDT with antimicrobials could help overcome the difficulty of fighting high level of resistance microorganisms and, as it is a multi-target approach, it could make the selection of resistant microorganisms more difficult.

Download Full-text